Systematic Position of Allium macrostemon Based on nrDNA  ITS and cpDNA trnL-F Sequence Data

doi:10.7677/ynzwyj201514174

Abstract

Abstract:

Allium macrostemon is an important medicinal and edible plant. Its systematic position and taxonmical classification remain controversial to date. To explore this issue, we reconstructed phylogenetic relationships among Amacrostemon and other related taxa using nrDNA ITS and cpDNA trnLF markers. The phylogenetic trees derived from Bayesian inference and maximumparsimony analysis showed that Amacrostemon is monophyletic, and has a close relationship with some species of polyphyletic sections Caerulea and Pallasia instead of sections Codonoprasum and Allium. The including of Amacrostemon within section Allium was not supported by both molecular data and morphological characters of spathe, filaments and ovary. Allium macrostemon should be included in a new section, however further studies using additional samples (especially those from Central Asia) is necessary. In addition, we also provided a discussion on the phylogenetic relationships among four original plants (Amacrostemon, Achinense, Acaeruleum and Aneriniflorum) of Allii Macrostemonis Bulbus and systematic position of partial species of section Pallasia.

Key words: Allium, A. macrostemon, ITS, trnL-F, Systematic position

摘要：

薤白 (Allium macrostemon) 是葱属中重要的药食同源种类，其系统地位和分类等级迄今尚无统一定论，一直存有争议。为了探讨其系统位置及其与相关类群的亲缘关系，本研究利用nrDNA ITS和cpDNA trnLF序列数据对该种及若干近缘类群进行了系统发育分析。基于贝叶斯法和最大简约法构建的系统树均表明薤白为一单系种，与多系的组Caerulea、Pallasia的部分种类有密切的系统发育关系，而与组Codonoprasum和Allium的种类关系疏远；结合总苞、花丝和子房等形态学特征，不支持将薤白划归Allium组或置于广义的Scorodon组。薤白可能代表一个新的组一级的类群，但尚待增加类群采样（尤其是中亚的种类）进一步研究。本文还在系统发育框架下探讨了中药薤白（Allii Macrostemonis Bulbus）的基源植物（薤白、藠头、棱叶韭和长梗韭）及Pallasia组部分物种的系统位置。

关键词: 葱属, 薤白, ITS, trnL-F, 系统位置

CLC Number:

Q 949

HUANG De-Qing, LI Qin-Qin, ZHOU Song-Dong, HE Xing-Jin. Systematic Position of Allium macrostemon Based on nrDNA ITS and cpDNA trnL-F Sequence Data[J]. Plant Diversity, 2015, 37(05): 537-545.

黄德青, 李琴琴, 周颂东, 何兴金. 基于nrDNA ITS和cpDNA trnL-F序列探讨薤白的系统地位[J]. Plant Diversity, 2015, 37(05): 537-545.

References

国家药典委员会, 2010. 中华人民共和国药典, 一部［M］. 北京: 中国医药科技出版社, 353
国家中医药管理局, 1999. 中华本草［M］. 上海: 上海科学技术出版社, V22: 30—36
许介眉, 1980. 葱属［A］// 汪发缵, 唐进. 中国植物志 (第14卷) ［M］. 北京: 科学出版社, 202—213
strm H, Hggstrm C, 2004. Generative reproduction in Allium oleraceum (Alliaceae) ［J］. Annales Botanici Fennici, 41: 1—14
Bogdanovic′ S, Brullo C, Brullo S, et al., 2011. Allium cithaeronis Bogdanovic′, C. Brullo, Brullo, Giusso, Musarella & Salmeri (Alliaceae), a new species from Greece［J］. Candollea, 66: 377—382
Brullo S, Guglielmo A, Pavone P et al., 2001. Cytotaxonomical notes on some rare endemic species of Allium (Alliaceae) from Greece［J］. Caryologia, 54 (1): 37—57
Brullo S, Guglielmo A, Pavone P et al., 2008. Taxonomic study on Allium dentiferum Webb & Berthel. (Alliaceae) and its relations with allied species from the Mediterranean［J］. Taxon, 57 (1): 243—253
Burland TG, 2000. DNASTAR’s Lasergene sequence analysis software［J］. Methods in Molecular Biology, 132: 71—91
Choi HJ, Giussani LM, Jang CG et al., 2012. Systematics of disjunct northeastern Asian and northern North American Allium (Amaryllidaceae) ［J］. Botany, 90 (6): 491—508
Choi HJ, Oh BU, 2011. A partial revision of Allium (Amaryllidaceae) in Korea and northeastern China［J］. Botanical Journal of the Linnean Society, 167: 153—211
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue［J］. Phytochemistry Bulletin, 19: 11—15
Duchoslav M, 2011. Polyploid complex of Allium oleraceum L. in Europe［D］. Olomouc: Palack University, 1—117
Friesen N, 1995. The genus Allium L. in the flora of Mongolia［J］. Feddes Repertorium, 106: 59—81
Friesen N, Fritsch RM, Blattner FR, 2006. Phylogeny and new intrageneric classification of Allium L. (Alliaceae) based on nuclear rDNA ITS sequences［J］. Aliso, 22: 372—395
Fritsch RM, Friesen N, 2002. Evolution, domestication and taxonomy［A］// Rabinowitch HD, Currah L ed., Allium Crop Science: Recent Advances ［M］. Wallingford, UK: CABI Publishing, 5—30
Hanelt P, 1996. Taxonomic problems in Mediterranean Allium and relationships with nonMediterranean Allium groups［J］. Bacconea, 5: 259—265
Hanelt P, SchulzeMotel J, Fritsch RM et al., 1992. Infrageneric grouping of Alliumthe Gatersleben approach［A］// Hanelt P, Hammer K, Knüpffer H ed., The Genus Allium: Taxonomic Problems and Genetic Resources［M］. Gatersleben, Germany, 107—123
Hirschegger P, Jake J, Trontelj P et al., 2010. Origins of Allium ampeloprasum horticultural groups and a molecular phylogeny of the section Allium (Allium: Alliaceae) ［J］. Molecular Phylogenetics and Evolution, 54: 488—497
Jiao Y (焦阳), Yin HB (尹海波), Dong SS (董双双), 2010. Materia medica textual research of Allium chinense G. Don［J］. Journal of Liaoning University of TCM (辽宁中医药大学学报), 12 (7): 186—188
Kawano S, Nagai Y, 1975. The productive and reproductive biology of flowering plants, 1: Life history strategies of three Allium species in Japan［J］. Botanical Magazine, 281—318
Khassanov FO, 1997. Conspectus of the wild growing Allium species of Middle Asia［A］// ztürk M, Semen , Grk G ed., Plant Life in Southwest and Central Asia［M］. Izmir, Turkey: EGE University Press, 141—159
Khassanov FO, 2000. Subinfrageneric grouping in genus Allium subgenus Allium［A］// Ashurmetov O, Khassanov F, Salieva Y ed., Plant Life in Southwest and Central Asia［M］. Proceedings of the 5th international symposium, 1822 May 1998, Tashkent, Uzbekistan, 107—112
Koyigˇit M, zhatay N, 2012. Allium maraschicum sp. nov. (Alliaceae) from Turkey［J］. Nordic Journal of Botany, 30: 553—559
Li QQ, Zhou SD, He XJ et al., 2010. Phylogeny and biogeography of Allium (Amaryllidaceae: Allieae) based on nuclear ribosomal internal transcribed spacer and chloroplast rps16 sequences, focusing on the inclusion of species endemic to China［J］. Annals of Botany, 106: 709—733
Mes THM, Fritsch RM, Pollner S et al., 1999. Evolution of the chloroplast genome and polymorphic ITS regions in Allium subgenus Melanocrommyum［J］. Genome, 42: 237—247
Nguyen NH, Driscoll HE, Specht CD, 2008. A molecular phylogeny of the wild onions (Allium; Alliaceae) with a focus on the western North American center of diversity ［J］. Molecular Phylogenetics and Evolution, 47: 1157—1172
Nylander JAA, 2004. MrModeltest 22. Computer program and documentation distributed by the author［Z］. Uppsala: Evolutionary Biology Centre, Uppsala University
Ronquist F, Huelsenbeck JP, 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models［J］. Bioinformatics, 19: 1572—1574
Swofford DL, 2003. PAUP*: Phylogenetic Analysis Using Parsimony (* and other methods), version 40b10［CP］. Sunderland: Sinauer Associates
Tamura K, Peterson D, Peterson N et al., 2011. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods［J］. Molecular Biology and Evolution, 28: 2731—2739
Xu JM, Kamelin RV, 2000. Allium L. // Wu ZY, Raven PH ed., Flora of China［M］, Vol 24. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 165—202
Zhao LL (赵露露), Zhang ZZ (张珍珍), Chen LL (陈玲玲) et al., 2012. Discovery of Heptaploid Allium macrostemon in Daluo Mountain, Zhejiang and the preliminary analysis on its formation mechanism［J］. Acta Botanica BorealiOccidentalia Sinica (西北植物学报), 32 (11): 2206—2210
Zhu SM (朱世梅), Xu JM (许介眉), 1999. Karyotypic differentiation in Allium macrostemon Bunge［J］. Acta Phytotaxonomica Sinica (植物分类学报), 37: 269—278
Zhu ZY (祝之友), 2000. Textual research primary origin and characteristic of Allium chinese G. Don［J］. Primary Journal of Chinese Materia Medica (基层中药杂志), 14 (6): 38—39

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Systematic Position of Allium macrostemon Based on nrDNA ITS and cpDNA trnL-F Sequence Data

基于nrDNA ITS和cpDNA trnL-F序列探讨薤白的系统地位

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