Identification of Salvia Species by nrDNA ITS and cpDNA rpl16 Sequence Analyses

doi:10.3724 SP.J.1143.2008.07224

Plant Diversity ›› 2008, Vol. 30 ›› Issue (03): 345-350.DOI: 10.3724 SP.J.1143.2008.07224

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Identification of Salvia Species by nrDNA ITS and cpDNA rpl16 Sequence Analyses

GUO Hui-Fang¹ , KAN Xian-Zhao², ZHANG Ren¹ ^{, 3} , CHEN Hong-Shan¹

1 Institute of Medicinal Biotechnology , Chinese Academy of Medical Sciences & Peking Union of Medical College, Beijing 100050, China; 2 State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany , Chinese Academy of Sciences , Beijing 100093, China ; 3 School of Biological Sciences, University of Wollongong, NSW 2522, Australia

Received:2007-09-29 Online:2008-06-25 Published:2008-06-25

nrDNA ITS 和cpDNA rpl16 基因测序法对六种鼠尾草的检测

郭会芳¹, 阚显照², 张韧^{1 , 3} , 陈鸿珊¹

1 中国医学科学院北京协和医学院医药生物技术研究所, 北京 100050;
2 中国科学院植物研究所系统与进化植物学国家重点实验室, 北京 100093 ;
3 卧龙岗大学生命科学院, 澳大利亚新南威尔士卧龙岗 2522

Abstract

Abstract: Three DNA regions were sequenced for testing six fresh plant samples of Salvia species . These three DNA regions were nrDNA ITS ( nuclear ribosomal DNA internal transcribed spacer), chloroplast rpl16 ( the gene encoding ribosomal protein L16 ) , and trnL- trnF ( the cpDNA region comprising the trnL and the intergenic spacer between trnL and trnF) . The results showed that the nrDNA ITS and rpl16 genes could provide novel information for origin identification of Salvia species. Due to their higher mutation rates of these 2 gene markers, Salvia species-specific primers were designed and S. miltiorrhiza and S. yunnanensis were identified. The trnL- trnF gene expressed low mutation rate, it could not identify the species . Since the damage of DNA by the pretreatments of the dry roots of Chinese herbs, it is hard to apply the
molecular markers to commercial samples for identification.

Key words: Salvia

摘要： 为从鼠尾草属植物中鉴别丹参品种, 采用基因测序方法, 用核糖体核酸内转录间隔区基因( nrDNA ITS) , 编码核蛋白体大亚基多肽L16 的基因( rpl16 ) 及叶绿体DNA 上包含trnL 以及trnL 和trnF 间隔区的区域基因( trnL- trnF) 的序列, 检测六种鼠尾草属新鲜植物。由于nrDNA ITS 和rpl16 突变率较高, 可以做为6 种鼠尾草的基源鉴定标记, 依此设计了两对特异引物, 从6 种鼠尾草中鉴定出丹参( Salvia miltiorrhiza)和云南鼠尾草( S. yunnanensis)。但trnL- trnF 突变率太低, 未能用于鉴别。商品干燥中药材因加工和储藏的方式致使DNA 降解严重, 基因测序法难于应用。

关键词: 鼠尾草属, ITS 基因, rpl16 基因, trnL- trnF 基因

CLC Number:

Q 945

GUO Hui-Fang , KAN Xian-Zhao, ZHANG Ren ^, , CHEN Hong-Shan. Identification of Salvia Species by nrDNA ITS and cpDNA rpl16 Sequence Analyses[J]. Plant Diversity, 2008, 30(03): 345-350.

郭会芳1 , 阚显照2 , 张韧1 , 3 , 陈鸿珊1 . nrDNA ITS 和cpDNA rpl16 基因测序法对六种鼠尾草的检测[J]. Plant Diversity, 2008, 30(03): 345-350.

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Identification of Salvia Species by nrDNA ITS and cpDNA rpl16 Sequence Analyses

nrDNA ITS 和cpDNA rpl16 基因测序法对六种鼠尾草的检测

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