Abstract: Physiological and chemical variation and construction of floral buds of cotyledons of Cucuibita pepo L . induced by kinetin are reported in the present paper . The results domonestrated that differentiation in floral bud of the cotyledons which cultured on MS mixed with kinetin 1 . 0 mg·L- 1 accounted for 45% of the total, whereas only reached 5% in the control . During construction of the floral buds , the endogenous soluble protein , soluble suger , RNA and POD activity of the cotyledons apparent exceeded
those of the control ; the ratio between Spd and Put (Spd􊄯Put ) is two times more than that of the control ; the content of starch and DNA are not quite different from the control , but DAP and Cad apparently decreased. It is easy to come to a conclusion that the varition of physiological and chemical induces in the cotyledons in vitro induced by kinetin promoted the construction of floral buds .

Key words: Cucuibita pepo

摘要： 报道了在激动素的影响下离体培养的西葫芦(Cucuibita pepo L .) 子叶的生理生化变异及花芽的构建。结果如下: 在附加有激动素(KT) 1 .0 mg·L- 1 的MS 培养基培养的西葫芦子叶中花芽的发生率占46% , 而对照组中仅达到5%。在花芽的构建过程中子叶内在的可溶蛋白, 可溶糖, RNA 和POD 活性明显高于对照组; 亚精胺(Spd) 和腐胺(Put ) 的比值(Spd/Put) 高于对照组的2 倍; 淀粉和DNA 的含量与对照组无大的区别, 但是二氨基丙烷(DAP)和尸胺(Cad) 却明显下降了。由此可见在激动素影响下子叶出现的生理生化指数变化促进了花芽的构建。

关键词: 西葫芦,  , 花芽构建, 激动素, 多胺FONT