Abstract: Tender leaves of Stylosanthes guianensis cv . Reyan Ⅱ were transformed with Agrobacterium tumefaciens LBA4404 carrying the gene VP1 coding food-and-mouth disease virus outer capsid protein . After 3 times kanamycin-resistant selection , the regenerated plantlets were analyzed further by PCR, PCRSouthern blot and Southern blot, the results confirmed that the VP1 gene were integrated in the genome . The results of RT-PCR and Northern blot of transgenic plants showed that VP1 gene was transcribed in transgenic plants .

Key words: FMDV

摘要： 将口蹄疫病毒外壳蛋白VP1 基因克隆到植物表达载体pBI121 , 并转化到根癌农杆菌
(Agrobacterium tumefaciens) 菌株LBA4404 中, 采用叶盘转化法转化柱花草(Stylosanthes spp .) 栽培品种热研二号柱花草(S. guianensis cv . Reyan Ⅱ ) , 获得了转基因植株, 经PCR、PCRSouthern blot 和Southern blot 分析表明: VP1 基因已整合到转基因柱花草植株的核基因组中。经RT-PCR、Northern blot 分析表明: VP1 基因已在转基因柱花草中获得转录。

关键词: 口蹄疫病毒, 柱花草,  , VP1 基因, 遗传转化FONT