Abstract: IGS1 genes were obtained by PCR amplification from genome DNA of Tricholoma matsutake fruit bodies collected from 16 counties in Yunnan . These gene fragments were digested by restriction endonuclease Cfr13Ⅰ . Two distinct IGS1 rDNA types were identified , namely A and C . Among the 127 fruit bodies investigated , 126 belong to type A while one from Jianchuan (T89) belongs to type C . Our results also showed that there are no significant heterogeneity in the IGS1 genes of these fruit bodies , except double CTTTs found in representative IGS1 gene of type A . The primary IGS1 RFLP patterns of Tricholoma matsutake from Yunnan are very similar to those from Japanese , implying that Tricholoma matsutake of these two areas are likely to be homologous .

Key words: Tricholoma matsutake

摘要： 应用Cfr13 I 限制性内切酶对采自于云南省16 个市县的127 个松茸子实体进行了IGS1-RFLP 比较分析, 发现126 个松茸子实体属于A 类型, 来自大理白族自治州剑川县的1 例(TF89) 为C 类型。通过对A类型的IGS1 序列分析发现产自云南的松茸有一个CTTT 的简单重复, 滇产松茸IGS1 序列差异不明显。滇产松茸的IGS1-RFLP 与日产松茸的主要类型十分相似, 两地松茸可能是同源的。

关键词: 松茸, 云南, IGS1-RFLP 酶切类型, IGS1 序列FONT