rDNA ITS ( ITS1 and ITS2 ) fragments of the basidiocarps, anamorph blastospores and mycelia isolates from Tremella aurantialba were amplified by PCR and then were sequenced . The results showed that ITS fragment of basidiocarps contained two bands with different number of bases: one was identical with ITS sequences of the anamorph blastospore isolates, and another was identical with ITS sequences of the mycelium isolates . Phylogenetic analysis based on ITS1 and ITS2 sequences indicated that the anamorph blastospore isolates is Tremella aurantialba and the mycelia isolates, the host fungus of T. aurantialba , is Stereum hirsutum . Phylogenetic trees were constructed by using sequences of T. aurantialba and those of its analog species T. aurantia , T. encephala and T. mesenterica in GenBank . All the results revealed that T. aurantialba is an independent species , which is consistent with the morphological evidence .
A novel motor protein , dynamin- like protein from day lily, was purified and identified by FPLC . Here we report its biochemical characterization . The molecular weight of the dynamin-like protein is 100 kD on SDS-PAGE. Isoelectric points are about 6. 15 and 6 .80 . The fluorescence emission wave length of dynamin-like protein is 346 nm by excitation at 280 nm . Through fluorescence spectra analysis, ultraviolet absorption spectrum and derivative spectrum, we conclude that it contains tryptophan and tyrosine residues . Pharmacological study indicates that mercapto may play an important role in
enzyme activity of dynamin-like protein .