Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)

doi:10.1016/j.pld.2024.09.009

Plant Diversity ›› 2025, Vol. 47 ›› Issue (01): 136-147.DOI: 10.1016/j.pld.2024.09.009

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Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)

Xin Wang^a,b, Qing-Hong Feng^a,c,d, Zhi-Hua Zeng^a,b, Zhi-Qiang Zhang^c, Jie Cai^a, Gao Chen^e, De-Zhu Li^a, Hong Wang^e, Wei Zhou^a,f,g

a. Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
b. University of Chinese Academy of Sciences, Beijing 100049, China;
c. Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650504, China;
d. Research Institute of Tropical Forestry, Yunnan Academy of Forestry and Grassland, Xishuangbanna, Yunnan 666102, China;
e. CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
f. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
g. Lijiang Forest Biodiversity National Observation and Research Station, Kunming Institute of Botany, Chinese Academy of Sciences, Lijiang, Yunnan 674100, China

Received:2024-07-24 Revised:2024-09-04 Online:2025-02-15 Published:2025-01-25
Contact: Hong Wang,E-mail:wanghong@mail.kib.ac.cn;Wei Zhou,E-mail:zhouwei@mail.kib.ac.cn
Supported by:
This research was supported by the Top-notch Young Talents Project of Yunnan Province (YNWR-QNBJ-2019-203), the National Natural Science Foundation of China (32470394), and the Key Basic Research Program of Yunnan Province (202201AS070057, 202101BC070003, and 202103AC100003).

Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)

Xin Wang^a,b, Qing-Hong Feng^a,c,d, Zhi-Hua Zeng^a,b, Zhi-Qiang Zhang^c, Jie Cai^a, Gao Chen^e, De-Zhu Li^a, Hong Wang^e, Wei Zhou^a,f,g

a. Germplasm Bank of Wild Species & Yunnan Key Laboratory of Crop Wild Relatives Omics, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
b. University of Chinese Academy of Sciences, Beijing 100049, China;
c. Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, Institute of Biodiversity, School of Ecology and Environmental Science, Yunnan University, Kunming, Yunnan 650504, China;
d. Research Institute of Tropical Forestry, Yunnan Academy of Forestry and Grassland, Xishuangbanna, Yunnan 666102, China;
e. CAS Key Laboratory for Plant Diversity and Biogeography of East Asia, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
f. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan 650201, China;
g. Lijiang Forest Biodiversity National Observation and Research Station, Kunming Institute of Botany, Chinese Academy of Sciences, Lijiang, Yunnan 674100, China

通讯作者: Hong Wang,E-mail:wanghong@mail.kib.ac.cn;Wei Zhou,E-mail:zhouwei@mail.kib.ac.cn
基金资助:
This research was supported by the Top-notch Young Talents Project of Yunnan Province (YNWR-QNBJ-2019-203), the National Natural Science Foundation of China (32470394), and the Key Basic Research Program of Yunnan Province (202201AS070057, 202101BC070003, and 202103AC100003).

Abstract

Abstract: Evolutionary transitions from sexual to asexual reproduction should have significant influences on genetic divergence and polymorphism at the genome level. Plant lineages with diverse reproductive systems provide opportunities to investigate this question using comparative approaches and studies of molecular evolution. We investigated evidence for differences among the transcriptomes of 19 Dioscorea species (wild yams) with diverse reproductive systems. These included sexual species, those that propagate primarily by bulbils, and those with mixed sexual and asexual reproductive modes. We examined how transitions between these reproductive systems affected between-species divergence and within-species polymorphism. Primarily asexual species exhibited a reduced efficacy of natural selection and accumulation of deleterious mutations for both divergence and polymorphism. In contrast, species with mixed reproductive strategies involving both seed and clonal reproduction showed no evidence of an increased fixation of harmful mutations at the divergence level, while an accumulation of genetic load present in polymorphism was evident. Our study indicates that the genetic consequences of evolutionary transitions from sexual to predominantly clonal reproduction is likely to depend on both the duration and extent of asexuality occurring in populations.

Key words: Asexual, Deleterious mutations, Dioscorea, Molecular evolution, Selection

摘要： Evolutionary transitions from sexual to asexual reproduction should have significant influences on genetic divergence and polymorphism at the genome level. Plant lineages with diverse reproductive systems provide opportunities to investigate this question using comparative approaches and studies of molecular evolution. We investigated evidence for differences among the transcriptomes of 19 Dioscorea species (wild yams) with diverse reproductive systems. These included sexual species, those that propagate primarily by bulbils, and those with mixed sexual and asexual reproductive modes. We examined how transitions between these reproductive systems affected between-species divergence and within-species polymorphism. Primarily asexual species exhibited a reduced efficacy of natural selection and accumulation of deleterious mutations for both divergence and polymorphism. In contrast, species with mixed reproductive strategies involving both seed and clonal reproduction showed no evidence of an increased fixation of harmful mutations at the divergence level, while an accumulation of genetic load present in polymorphism was evident. Our study indicates that the genetic consequences of evolutionary transitions from sexual to predominantly clonal reproduction is likely to depend on both the duration and extent of asexuality occurring in populations.

关键词: Asexual, Deleterious mutations, Dioscorea, Molecular evolution, Selection

Xin Wang, Qing-Hong Feng, Zhi-Hua Zeng, Zhi-Qiang Zhang, Jie Cai, Gao Chen, De-Zhu Li, Hong Wang, Wei Zhou. Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)[J]. Plant Diversity, 2025, 47(01): 136-147.

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Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)

Effects of mode of reproduction on genetic polymorphism and divergence in wild yams (Dioscoreaceae: Dioscorea)

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