Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae)

doi:10.7677/ynzwyj201413109

Abstract

Abstract:

Three types of intermediate morphological individuals were found in the sympatric distribution areas of Ligularia vellerea and Lsubspicata. These intermediate individuals were hypothesized to be the hybrids of these two species based on the detailed comparison of their morphological characters. To test this hypothesis, we compared the DNA sequence of Lvellerea, Lsubspicata and the intermediate individuals by using the internal transcribed spacer region of nuclear ribosomal and atpBrbcL intergenic spacer region of the chloroplast. Direct sequencing of ITS clearly had additives and then cloned sequencing cloned out two kinds of sequences that were identical to those putative parental species, thus these putative hybrids were approved. With different morphological characters among three types, their plastid donors were all Lsubspicata according to the maternal sequences of atpBrbcL.

Key words: Ligularia vellerea, L.subspicata, Hybrid progeny, ITS, atpB-rbcL

摘要：

在棉毛橐吾(Ligularia vellerea)和穗序橐吾(Lsubspicata)同域分布的居群中存在形态特征介于两者之间的个体。经分析，这些个体有3种不同的形态类型，可能是这两个物种之间的杂交后代。为检验这一假设，对所采集的假设亲本和中间个体材料进行了核糖体内转录间隔区ITS45和叶绿体atpBrbcL测序。假设杂交个体的ITS直接测序结果具明显的叠加性，之后的克隆测序也分离出两种与假设亲本种相同的序列类型，因此，这些假设杂交个体得到了证实。据母系遗传的叶绿体atpBrbcL测序结果，穗序橐吾同是它们的质体贡献者，即虽然这些杂交个体具不同的形态特征，但都是以棉毛橐吾和穗序橐吾为亲本且以穗序橐吾为质体贡献者的杂交后代。

关键词: 棉毛橐吾, 穗序橐吾, 杂交后代, 核ITS4-5, 叶绿体atpB-rbcL片段

CLC Number:

Q 321.1
Q 78

YU Jiao-Jun, PAN Li, PAN Yue-Zhi, GONG Xun. Natural Hybrids between Ligularia vellerea and L.subspicata (Asteraceae: Senecioneae)[J]. Plant Diversity, 2014, 36(02): 219-226.

余姣君, 潘莉, 潘跃芝, 龚洵. 棉毛橐吾和穗序橐吾的自然杂交研究[J]. Plant Diversity, 2014, 36(02): 219-226.

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Appendix: List of accessions of Ligularia included in the phylogenetic analysis of nuclear rDNA ITS and atpBrbcL sequences. GenBank numbers for all accessions are provided.
Accessions, GenBank No.
ITS: Ligularia platyglossa, AY458841; Lstenoglossa, AB523365; Ljaponica, AY458825; Latroviolacea, DQ272322; Lthyrsoidea, DQ272320; Ltaquetii, GU323282; Lkongkalingensis, DQ272343; Lcurvisquama, DQ272324; Lcyathiceps, DQ272328; Llatihastata, DQ272334.
atpBrbcL: Lfranchetiana, AB375447; Loligonema, AB523847; Lanoleuca, AB557885; Lveitchiana, AB557887; Ligularia kanaitzensis var. subnudicaulis, AB281495.

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