Phylogeographical Study on Eriophyton wallichii (Labiatae) from Alpine Scree of QinghaiTibetan Plateau

doi:10.3724/SP.J.1143.2011.11082

Abstract

Abstract:

In this study, we examined the nuclear ribosomal DNA internal transcribed spacer (ITS) sequence variation of Eriophyton wallichii, a perennial endemic to alpine scree of the QinghaiTibetan Plateau. Nineteen haplotypes were detected by analyzing 187 individuals from 20 populations of E.wallichii. Most populations hold the unique haplotype, which showed different population with different haplotype. An analysis of molecular variance (AMOVA) for populations showed that the genetic variation mainly resided among populations (89.54%), the level of differentiation among populations was very high (GST=0.863; NST=0.957) but didn’t show a significant phylogeographical structure. It is different from foregoing other species reported in QinghaiTibetan Plateau that the haplotypes of E.wallichii showed a distribution pattern that almost one population hold one unique haplotype, hardly share same halotype among populations.We hypothesized the unique genetic structure of E.wallichii might result from allopatric fragmentation in the “islands of alpine” due to the extremely isolated alpine scree and rough topography of the region, through the Quaternary climate oscillation dramatically caused population expanding or shrinking.

Key words: QinghaiTibetan Plateau, Alpine scree, Eriophyton wallichii, Phylogeography, ITS

摘要：

绵参 (Eriophyton wallichii) 是中国青藏高原地区高山流石滩特有植物。为探讨第四纪冰期气候波动对青藏高原高山流石滩植物居群遗传结构以及谱系历史的影响，检测了绵参20个居群187个个体的核糖体内转录间隔区 (ITS) 的序列变异，共发现19个单倍型，且结果表明大部分居群拥有独自的单倍型。AMOVA分析表明，居群间的变异占了总变异量的89.54%，居群遗传分化指数很高 (GST=0.863，NST=0.957)，但无明显的谱系结构。我们推测这种独特的谱系地理结构可能主要是由于高山流石滩独特的地形和环境及“孤岛效应”形成的，与先前研究过的青藏高原植物不同，绵参呈现出高度分化的遗传结构，常常呈现出“一个居群，一种单倍型”的模式，这可能是由于第四纪冰期及间冰期尽管绵参的分布范围有扩大和收缩，但始终由于特殊的高山生境和流石滩基质，导致了该物种长期以来居群间很少有基因交流并一直处于隔离状态，造成了现在这样的谱系地理结构。

关键词: 青藏高原, 高山流石滩, 绵参, 谱系地理学, ITS

CLC Number:

Q 948

WANG Xiao-Xiong-, YUE Ji-Pei-, SUN Hang-, LI Zhi-Min. Phylogeographical Study on Eriophyton wallichii (Labiatae) from Alpine Scree of QinghaiTibetan Plateau[J]. Plant Diversity, 2011, 33(6): 605-614.

王晓雄1、2, 乐霁培2, 孙航2, 李志敏1. 青藏高原高山流石滩特有植物绵参的谱系地理学研究[J]. Plant Diversity, 2011, 33(6): 605-614.

References

刘腾靓, 2009. 西藏沙棘的谱系地理学研究［D］. 兰州: 兰州大学(硕士学位论文)
吴征镒, 朱彦承主编, 1985. 云南植被［M］. 北京: 科学出版社
Bandelt HJ, Forster P, Rohl A. 1999. Medianjoining networks for inferring intraspecific phylogenies［J］. Molecular Biology and Evolution, 16: 37—48
Chen SY, Wu GL, Zhang DJ et al., 2008. Potential refugium on the QinghaiTibet Plateau revealed by the chloroplast DNA phylogeography of the alpine species Metagentiana striata (Gentianaceae) ［J］. Botanical Journal of the Linnean Society, 157: 125—140
Collevatti RG, Rabelo SG, Vieira RF, 2009. Phylogeography and disjunct distribution in Lychnophora ericoides (Asteraceae), an endangered cerrado shrub species［J］. Annals of Botany, 104: 655—664
Comes HP, Kadereit JW, 1998. The effects of Quatenary climatic changes on plant distribution and evolution［J］. Trends in Plant Science, 3: 432—438
Doyle JJ, Doyle JL, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf material［J］. Phytochemical Bulletin, 9: 11—15
Duan YZ (段义忠), Zhang DJ (张得钧), Gao QB (高庆波) et al., 2010. Characteristic of molecular evolution of Sibiraea angustata based on nrDNA ITS and cpDNA trnLF sequence analysis［J］. Bulletin of Botanical Research (植物研究), 30 (2): 146—151
Dynesius M, Jansson R, 2000. Evolutionary consequences of changes in species’ geographical distributions driven by Milankovitch climate oscillations［J］. Proceedings of the National Academy of Sciences, 97: 9115—9120
Excoffier LP, Laval G, Schneider S, 2006. Arlequin 3.01: An Integrated Software Package for Population Genetics Data Analysis［M］. Switzerland: Institute of Zoology, University of Berne
Forster P, Bandelt HJ, Rhl A et al., 2004. Network 4.2.0.1. Software available free at www.fluxusengineering.com. Fluxus Technology Ltda
Fujii N, Senni K, 2006. Phylogeography of Japanese alpine plants: biogeographic importance of alpine region of central Honshu in Japan［J］. Taxon, 55: 43—52
Fu YX, Li WH, 1993. Statistical tests of neutrality of mutations［J］. Genetics, 133: 693—709
Gao LM, Moller M, Zhang XM et al., 2007. High variation and strong phylogeographic pattern among cpDNA haplotypes in Taxus wallichiana (Taxaceae) in China and North Vietnam［J］. Molecular Ecology, 16: 4684—4698
Gao QB, Zhang DJ, Chen SY et al., 2009. Chloroplast DNA phylogeography of Rhodiola alsia (Crassulaceae) in the QinghaiTibet Plateau［J］. Botany, 87 (11): 1077—1088
Hewitt G, 2000. The genetic legacy of the Quaternary ice ages［J］. Nature, 405: 907—913
Ikeda H, Senni KEI, Fujii N et al., 2006. Refugia of Potentilla matsumurae (Rosaceae) located at high mountains in the Japanese archipelago［J］. Molecular Ecology, 15: 3731—3740
Ikeda H, Setoguchi H, 2007. Phylogeography and refugia of the Japanese endemic alpine plant, Phyllodoce nipponica Makino (Ericaceae)［J］. Journal of Biogeography, 34: 169—176
Koch MA, Kiefer C, Ehrich D et al., 2006. Three times out of Asia Minor: the phylogeography of Arabis alpina L. (Brassicaceae)［J］. Molecular Ecology, 15: 825—839
Kropf M, Kandereit JW, Comes HP, 2003. Differential cycles of range contraction and expansion in European high mountain plants during the late Quaternary: insight from Pritzelago alpine (L) D. Kuntze (Brassicaceae) ［J］. Molecular Ecology, 12: 931—949
Li BS (李渤生), Zhang JW (张经纬), Wang JT (王金亭) et al., 1981. A primary study of subnival vegetation in Xizang［J］. Acta Botanical Sinica (植物学报), 23 (2): 132—139
Li C, Shimono A, Shen HH et al., 2010. Phylogeography of Potentilla fruticosa, an alpine shrub on the QinghaiTibetan Plateau［J］. Journal of Plant Ecology, 3: 9—15
Liu LH (刘伦辉), Yu YD (余有德), Zhang JH (张建华), 1984. The division of vertical vegetation zone in Hengduanshan［J］. Acta Botanica Yunnanica (云南植物研究), 6 (2): 205—216
Mani MS, 1980. Introduction: The high altitude environment［A］. In: Mani MS, Giddings LE eds. Ecology of Highlands (Monographiae Biologicae Volume 40)［M］. The Netherlands: Dr W Junk by Publishers The HagueBostonLondon, 1—9
Mantel N, 1967. The detection of disease clustering and a generalized regression approach［J］. Cancer Research, 27 (2): 209—220
Meng LH, Yang R, Abbott Ill et al., 2007. Mitochondrial and chloroplast phylogeography of Picea crassifolia Kom (Pinaceae) in the QinghaiTibetan Plateau and adjacent highlands［J］. Molecular Ecology, 16: 4128—4137
Nei M, 1987. Molecular Evolutionary Genetics［M］. New York: Columbia University Press
Opgenoorth L, Vendramin GG, Mao K et al., 2010. Tree endurance on the Tibetan Plateau marks the world’s highest known tree line of the Last Glacial Maximum［J］. New Phytologist, 185: 332—342
Pons O, Petit RJ, 1996. Measuring and testing genetic differentiation with ordered versus unordered alleles［J］. Genetics, 144: 1237—1245
Qiu YX, Fu CX, Comes HP, 2011. Plant molecular phylogeography in China and adjacent regions: Tracing the genetic imprints of Quaternary climate and environmental change in the world’s most diverse temperate flora［J］. Molecular Phylogenetics and Evolution, 59 (1): 225—244
Raymond M, Rousset F, 1995. An exact test for population differentiation［J］. Evolution, 49: 1280—1283
Rogers AR, Harpending H, 1992. Population growth makes waves in the distribution of pairwise differences［J］. Molecular Biology and Evolution, 9: 552—569
Rozas J, SanchezDeI Barrie JC, Messeguer X et al., 2003. DNASP: DNA polymorphism analyses by the coalescent and other methods［J］. Bioinformatics, 19: 2496—2497
Rutherford S, D’Hondt S, 2000. Early onset and tropical forcing of 100000year Pleistocene glacial cycles［J］. Nature, 408: 72—75
Scheen AC, Bendiksby M, Ryding O et al., 2010. Molecular phylogenetics, character evolution, and suprageneric classification of Lamoideae (Lamiaceae)［J］. Annals of the Missouri Botanical Garden, 97: 191—217
Smouse PE, Long JC, Sokal RR, 1986. Multiple regression and correlation extensions of the Mantel test of matrix correspondence［J］. Systematic Biology, 35 (4): 627—632
Song MS (宋敏舒), Yue JP (乐霁培), Sun H (孙航) et al., 2011. Phylogeographical study on Primula poissonii (Primulaceae) from Hengduan Mountains［J］. Plant Diversity and Resources (植物分类与资源学报), 33 (1): 91—100
Swofford DL, 2003. PAUP*. Phylogenetic Analysis Using Parsimony (* and Other Methods). Version 4.0b10［M］. Sunderland, Massachusetts: Sinauer Associates
Taberlet P, Fumagalli L, WustSaucy AG et al., 1998. Comparative phylogeography and postglacial colonization routes in Europe［J］. Molecular Ecology, 7: 453—464
Tajima F, 1989. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism［J］. Genetics, 7: 381—397
Thompson JD, Gibson TJ, Plewniak F et al., 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools［J］. Nucleic Acids Research, 25: 4876—4882
Wang FY, Gong X, Hu CM et al., 2008. Phylogeography of an alpine species Primula secundiflora inferred from the chloroplast DNA sequence variation［J］. Journal of Systematics and Evolution, 46 (1): 13—22
Wang LY, Abbott RJ, Zheng W et al., 2009a. History and evolution of alpine plants endemic to the OinghaiTibetan Plateau: Aconitum gymnandrum (Ranunculaceae)［J］. Molecular Ecology, 18: 709—721
Wang LY, Ikeda H, Liu TL et al., 2009b. Repeated range expansion and glacial endurance of Potentilla glabra (Rosaceae) in the QinghaiTibetan Plateau［J］. Journal of Integrative Plant Biology, 51: 698—706
Weir BS, Cockerham CC, 1984. Estimating Fstatistics for the analysis of population structure［J］. Evolution, 38: 1358—1370
White TJ, Bruns T, Lee S et al., 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics［A］. In: Innis MA, Gelfand DH, Sninsky JJ et al. eds. PCR Protocols: A Guide to Methods and Application［M］. San Diego, Ca
lifornia: Academic Press, 315—322
Winograd IJ, Landwehr JM, Ludwig KR et al., 1997. Duration and structure of the past four interglaciations［J］. Quaternary Research, 48: 141—154
Wu CY (吴征镒), Li HW (李锡文), 1977. Labiatae［A］. In: Flora Reipublicae Popularis Sinicae［M］. Beijing: Science Press, 65: 1—649
Yang FS, Li YF, Ding X et al., 2008. Extensive population expansion of Pedicularis longiflora (Orobanchaceae) on the OinghaiTibetan Plateau and its correlation with the Quaternary climate change［J］. Molecular Ecology, 17: 5135—5145
Zhang Q, Chiang TY, George M et al., 2005. Phylogeography of the QinghaiTibetan plateau endemic Juniperus przewalskii (Cupressaceae) inferred from chloroplast DNA sequence variation［J］. Molecular Ecology, 14: 3513—3524
Zhang YH, Volis S, Sun H, 2010. Chloroplast phylogeny and phylogeography of Stellera chamaejasme on the QinghaiTibet Plateau and in adjacent regions［J］. Molecular Phylogenetics and Evolution, 57 (3): 1162—1172

[1]	Zhi-Chao Li, Chao-Qun Xu, Wei Zhao, Shuai Nie, Yu-Tao Bao, Hui Liu, Zhen Xing, Jian-Feng Mao, Xiao-Ru Wang. Ecophysiological transition mediated by hybridization in a hybrid pine species complex [J]. Plant Diversity, 2025, 47(04): 604-619.
[2]	Yu-Mei Yan, Ze-Xin Fan, Pei-Li Fu, Zhi-Yong Zhang. Drought tolerance traits explain differential stem growth rates of evergreen and deciduous trees in a tropical karst forest [J]. Plant Diversity, 2025, 47(03): 454-465.
[3]	Xue Wang, Xinrui Liu, Shuang Chen, Jiang Zhu, Yanqi Yuan, Rong Zhu, Kaixi Chen, Xue Yang, Xiaochun Wang, Weiyi Mo, Ruili Wang, Shuoxin Zhang. Elevational variation in anatomical traits of the first-order roots and their adaptation mechanisms [J]. Plant Diversity, 2025, 47(02): 291-299.
[4]	Wenjie Guo, Lu Gong, Yan Luo, Qian Guo. Does season regulate heterochronous leaf growth? Mechanisms of petiole-lamina trade-offs in broad-leaved woody plants of the Tianshan Mountains [J]. Plant Diversity, 2024, 46(06): 755-765.
[5]	Tana Wuyun, Lu Zhang, Tiina Tosens, Bin Liu, Kristiina Mark, José ángel Morales-Sánchez, Jesamine Jöneva Rikisahedew, Vivian Kuusk, ülo Niinemets. Extremely thin but very robust: Surprising cryptogam trait combinations at the end of the leaf economics spectrum [J]. Plant Diversity, 2024, 46(05): 621-629.
[6]	Jingya Zhang, Cui Xiao, Xiaoyu Duan, Xin Gao, Hao Zeng, Rong'an Dong, Gang Feng, Keping Ma. Species' geographical range, environmental range and traits lead to specimen collection preference of dominant plant species of grasslands in Northern China [J]. Plant Diversity, 2024, 46(03): 353-361.
[7]	Rong Ma, Qi Xu, Yongqian Gao, Deli Peng, Hang Sun, Bo Song. Patterns and drivers of plant sexual systems in the dry-hot valley region of southwestern China [J]. Plant Diversity, 2024, 46(02): 158-168.
[8]	Ju Li, Shu-Bin Zhang, Yang-Ping Li. Photosynthetic response dynamics in the invasive species Tithonia diversifolia and two co-occurring native shrub species under fluctuating light conditions [J]. Plant Diversity, 2024, 46(02): 265-273.
[9]	Ming-Yue Jin, Daniel J. Johnson, Guang-Ze Jin, Qing-Xi Guo, Zhi-Li Liu. Soil water content and nitrogen differentially correlate with multidimensional leaf traits of two temperate broadleaf species [J]. Plant Diversity, 2023, 45(06): 694-701.
[10]	Yan Ke, Feng-Ping Zhang, Yun-Bing Zhang, Wei Li, Qin Wang, Da Yang, Jiao-Lin Zhang, Kun-Fang Cao. Convergent relationships between flower economics and hydraulic traits across aquatic and terrestrial herbaceous plants [J]. Plant Diversity, 2023, 45(05): 601-610.
[11]	Amarpreet Kaur, Shalinder Kaur, Harminder Pal Singh, Daizy R. Batish. Is intraspecific trait differentiation in Parthenium hysterophorus a consequence of hereditary factors and/or phenotypic plasticity? [J]. Plant Diversity, 2023, 45(05): 611-620.
[12]	Korina Ocampo-Zuleta, Ángela Parrado-Rosselli. Functional diversity in an Andean subpáramo affected by wildfire in Colombia [J]. Plant Diversity, 2023, 45(04): 385-396.
[13]	Xue Li, Markus Ruhsam, Yi Wang, Hong-Ying Zhang, Xiao-Yan Fan, Lei Zhang, Jing Wang, Kang-Shan Mao. Wind-dispersed seeds blur phylogeographic breaks: The complex evolutionary history of Populus lasiocarpa around the Sichuan Basin [J]. Plant Diversity, 2023, 45(02): 156-168.
[14]	Harshita Bhatia, Gaurav Srivastava, R. C. Mehrotra. Legumes from the Paleocene sediments of India and their ecological significance [J]. Plant Diversity, 2023, 45(02): 199-210.
[15]	Chao Liu, Huan-Huan Chen, Li-Zhou Tang, Phyo Kay Khine, Li-Hong Han, Yu Song, Yun-Hong Tan. Plastid genome evolution of a monophyletic group in the subtribe Lauriineae (Laureae, Lauraceae) [J]. Plant Diversity, 2022, 44(04): 377-388.