Plant Diversity ›› 2008, Vol. 30 ›› Issue (04): 457-463.DOI: 10.3724 SP.J.1143.2008.07228
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WANG Yi-Qun
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王逸群
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Abstract: The plant expression vector containing human alpha-lactalbumin gene was constructed and its gene was transformed into tobacco using leaf disc mediated by Agrobacterium tumefaciens LBA 4404 as a vehicle. PCR and Southern blot analyses showed that human alpha-lactalbumin gene was integrated into the tobacco genome. RT-PCR and histochemical staining for GUS activity demonstrated that human alpha-lactalbumin gene was expressed in transgenic tobacco plants. Further analysis of the data indicated that the average of cysteine contents in 9 transgenic tobacco plants was greatly increased by 166.40% besides one of them which was increased by 318.02%
Key words: Human alpha-lactalbumin gene
摘要： 将人α-乳清白蛋白基因构建到植物表达载体上, 通过农杆菌介导采用叶盘法将人α-乳清白蛋白基因导入到烟草中, 经过PCR 和Southern blot 分析表明: 人α-乳清白蛋白基因已经整合到烟草基因组中; 经过RT-PCR 和GUS 组织化学染色证明: 人α-乳清白蛋白基因得到了表达。测定了9 株转基因烟草叶片半胱氨酸含量, 大部分植株有着明显的提高, 最高幅度达到了318.02% , 半胱氨酸含量平均提高了166.40%。
关键词: 人α-乳清白蛋白基因, 转基因烟草, 半胱氨酸, 提高
CLC Number:
Q 758
WANG Yi-Qun. Transformation of Human Alpha-lactalbumin Gene into Tobacco and Improvement of Cysteine Contents in Transgenic Tobacco Plants[J]. Plant Diversity, 2008, 30(04): 457-463.
王逸群 . 转人α-乳清白蛋白基因烟草中半胱氨酸含量的提高[J]. Plant Diversity, 2008, 30(04): 457-463.
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URL: https://www.integrativebiology.ac.cn/pd/EN/10.3724 SP.J.1143.2008.07228
https://www.integrativebiology.ac.cn/pd/EN/Y2008/V30/I04/457
