Abstract: In this paper , plumules, cotyledons, hypocotyls, blades, petioles and stalks of physic nut ( Jatropha curcas L. ) were used as explants, and callus induction and plant regeneration were studied on MS medium contained different concentrations of 6-BA and NAA. The results showed that the MS medium with 5.0 mg/L BA and 1.0 mg/L NAA was the best for callus induction, and with 5.0mg/L BA and 0.1mg/L NAA, for formation of adventitious bud, and with 1.0mg/L BA and 1.0 mg/L NAA, for bud growth, and that 1􊄯2 MS medium with 1.0 mg/L NAA was the best for formation and growth of adventitious root .

Key words: Callus

摘要： 以小桐子( Jatropha curcas) 的胚芽、子叶、下胚轴、叶柄、叶片和茎段作为外植体, 用不同浓度的6-苄基腺嘌呤(6-BA) 和α-萘乙酸(NAA) 对其进行愈伤组织的诱导和植株再生的研究。结果表明: 在MS培养基中加入5.0 mg/L 6-BA 和1.0 mg/L NAA 对愈伤组织的诱导效果最好; 加入5.0 mg/L 6-BA 和0.1 mg/L NAA 对不定芽的诱导最为有效, 加入0.1 mg/L 6-BA 和1.0 mg/L NAA 有利于芽的生长; 加入1 . 0 mg/L NAA
的1􊄯2 MS 培养基对生根最为有利。

关键词: 小桐子, 种子, 外植体, 组织培养, 愈伤组织FONT