Phylogeography of Angelica nitida (Apiaceae), an endemic to the QinghaiTibet Plateau

doi:10.7677/ynzwyj201312178

Abstract

Abstract:

In this study, we examined nuclear ribosomal DNA internal transcribed spacer (ITS) sequence variation of Angelica nitida, a perennial plant species that is endemic to alpine scree in the QinghaiTibetan Plateau. Seven ITS haplotypes were detected among 147 individuals sampled across 16 populations. All populations except one (population SP) contained haplotype (H1). However, analysis of molecular variance (AMOVA) showed that ITS variation was mainly present among populations (67%) and that significant phylogeographical structure was evident (NST>GST, P<0.05). We inferred from our results that the current distribution pattern of A.nitida resulted from range retreats and expansions during the Quaternary. During Quaternary glaciations A.nitida retreated to refugia at the edge of the QinghaiTibet Plateau , but then expanded its range during interglacials and the current postglacial period.

Key words: Angelica nitida, Phylogeography, Quaterary, Founder effect, ITS

摘要：

为探讨第四纪冰期气候波动对青藏高原多年生草本植物青海当归地理分布格局形成的影响，检测了青海当归16个居群147个个体的核糖体内转录间隔区(ITS)的序列变异，共发现了7个单倍型。结果表明除松潘居群外其余15个居群都拥有单倍型H1，青藏高原边缘的居群拥有6种ITS单倍型，其中5种为私有单倍型。谱系关系分析显示单倍型H1为较古老的单倍型，AMOVA分析表明，居群间的变异占了总变异量的67%，居群遗传分化显著，并且存在较明显的谱系地理结构（NST>GST，P<0.05)。我们认为青海当归在第四纪冰期最大一次冰期来临时退缩到青藏高原边缘的避难所，冰期后在扩张过程中经历了奠基者效应形成了如今的分布格局。

关键词: 青海当归, 谱系地理, 第四季, 奠基者效应, ITS

CLC Number:

Q 948

ZHANG Xue-Mei, HE Xing-Jin. Phylogeography of Angelica nitida (Apiaceae), an endemic to the QinghaiTibet Plateau[J]. Plant Diversity, 2013, 35(4): 505-512.

张雪梅, 何兴金. 青藏高原特有植物青海当归的谱系地理学初探[J]. Plant Diversity, 2013, 35(4): 505-512.

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