Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

doi:10.1016/j.pld.2022.10.004

Plant Diversity ›› 2023, Vol. 45 ›› Issue (03): 326-336.DOI: 10.1016/j.pld.2022.10.004

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Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

Yu-Wen Zhang^a,b,c, Yu-Cen Shi^a,b,c, Shi-Bao Zhang^a,b,d

a. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
b. Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, Yunnan, China;
c. University of Chinese Academy of Sciences, Beijing 100049, China;
d. Lijiang Forest Biodiversity National Observation and Research Station, Kunming Institute of Botany, Chinese Academy of Sciences, Lijiang 674100, Yunnan, China

Received:2022-07-05 Revised:2022-09-17 Online:2023-07-06
Contact: Shi-Bao Zhang,E-mail:sbzhang@mail.kib.ac.cn
Supported by:
This work is supported by the Project for Innovation Team of Yunnan Province (202105AE160012), the Project for Construction of International Flower Technology Innovation Center and Achievement Industrialization (2019ZG006), the Project for the Germplasm Bank of Wild Species, and the KC Wong Education Foundation, CAS, and the project for High-level Talent Training Plan of Yunnan Province.

Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

Yu-Wen Zhang^a,b,c, Yu-Cen Shi^a,b,c, Shi-Bao Zhang^a,b,d

a. Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
b. Yunnan Key Laboratory for Wild Plant Resources, Kunming 650201, Yunnan, China;
c. University of Chinese Academy of Sciences, Beijing 100049, China;
d. Lijiang Forest Biodiversity National Observation and Research Station, Kunming Institute of Botany, Chinese Academy of Sciences, Lijiang 674100, Yunnan, China

通讯作者: Shi-Bao Zhang,E-mail:sbzhang@mail.kib.ac.cn
基金资助:
This work is supported by the Project for Innovation Team of Yunnan Province (202105AE160012), the Project for Construction of International Flower Technology Innovation Center and Achievement Industrialization (2019ZG006), the Project for the Germplasm Bank of Wild Species, and the KC Wong Education Foundation, CAS, and the project for High-level Talent Training Plan of Yunnan Province.

Abstract

Abstract: Dendrobium nobile is an important medicinal and nutraceutical herb. Although the ingredients of D. nobile have been identified as polysaccharides, alkaloids, amino acids, flavonoids and bibenzyls, our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited. Here, we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D. nobile. A total of 1005 metabolites and 31,745 genes were detected in the stems of D. nobile. The majority of these metabolites and genes were involved in the metabolism of carbohydrates (fructose, mannose, glucose, xylulose and starch), while some were involved in the metabolism of secondary metabolites (alkaloids, β-tyrosine, ferulic acid, 4-hydroxybenzoate and chrysin). Our predicted regulatory network indicated that five genes (AROG, PYK, DXS, ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis. Correlation analysis identified that six genes (ALDO, PMM, BGLX, EGLC, XYLB and GLGA) were involved in carbohydrate metabolism, and two genes (ADT and CYP73A) were involved in secondary metabolite biosynthesis. Our analyses also indicated that phosphoenol-pyruvate (PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis. The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species.

Key words: Dendrobium nobile, Transcriptome, Metabolome, Polysaccharides, Alkaloids, Secondary metabolite biosynthesis

摘要： Dendrobium nobile is an important medicinal and nutraceutical herb. Although the ingredients of D. nobile have been identified as polysaccharides, alkaloids, amino acids, flavonoids and bibenzyls, our understanding of the metabolic pathways that regulate the synthesis of these compounds is limited. Here, we used transcriptomic and metabolic analyses to elucidate the genes and metabolites involved in the biosynthesis of carbohydrate and several secondary metabolites in the stems of D. nobile. A total of 1005 metabolites and 31,745 genes were detected in the stems of D. nobile. The majority of these metabolites and genes were involved in the metabolism of carbohydrates (fructose, mannose, glucose, xylulose and starch), while some were involved in the metabolism of secondary metabolites (alkaloids, β-tyrosine, ferulic acid, 4-hydroxybenzoate and chrysin). Our predicted regulatory network indicated that five genes (AROG, PYK, DXS, ACEE and HMGCR) might play vital roles in the transition from carbohydrate to alkaloid synthesis. Correlation analysis identified that six genes (ALDO, PMM, BGLX, EGLC, XYLB and GLGA) were involved in carbohydrate metabolism, and two genes (ADT and CYP73A) were involved in secondary metabolite biosynthesis. Our analyses also indicated that phosphoenol-pyruvate (PEP) was a crucial bridge that connected carbohydrate to alkaloid biosynthesis. The regulatory network between carbohydrate and secondary metabolite biosynthesis established will provide important insights into the regulation of metabolites and biological systems in Dendrobium species.

关键词: Dendrobium nobile, Transcriptome, Metabolome, Polysaccharides, Alkaloids, Secondary metabolite biosynthesis

Yu-Wen Zhang, Yu-Cen Shi, Shi-Bao Zhang. Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile[J]. Plant Diversity, 2023, 45(03): 326-336.

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Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

Metabolic and transcriptomic analyses elucidate a novel insight into the network for biosynthesis of carbohydrate and secondary metabolites in the stems of a medicinal orchid Dendrobium nobile

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