收稿日期: 2011-04-18
网络出版日期: 2011-05-19
基金资助
国家自然科学基金项目(30871548)
Development of EST-SSR Markers from Jatropha curcas (Euphorbiaceae) and Their Application in Genetic Diversity Analysis among Germplasms
Received date: 2011-04-18
Online published: 2011-05-19
Supported by
国家自然科学基金项目(30871548)
小桐子(Jatropha curcas) 适应性强，不择土壤，种子油脂性能适宜生物柴油的生产，是重要的生物柴油植物。基于小桐子种子发育过程中的EST序列，采用生物信息学方法，从4640个EST非冗余序列上鉴别了1009个SSR位点并分析其分布特征；开发了11对多态的EST-SSR分子标记，并利用这些分子标记调查了24个不同地理种源的遗传多样性，从每个位点的等位基因数目（2~3，平均为2.45）、期望杂合度（He为0.0887~0.5128，平均是0.2736）、多态信息含量（PIC为0.0847~0.4031，平均是0.2313）等方面反映了小桐子种质的遗传多样性低。进一步分析显示不同地理种源的遗传关系缺乏明显的地理结构。作者开发的EST-SSR分子标记不仅有助于小桐子种质的遗传多样性研究，也有助于小桐子种质间的遗传关系鉴别。
杨春1、2 , 刘爱忠1 . 小桐子EST-SSR分子标记的开发与种质遗传多样性分析[J]. Plant Diversity, 2011 , 33(5) : 529 -534 . DOI: 10.3724/SP.J.1143.2011.11064
Jatropha curcas (Euphorbiaceae) has created tremendous interest all over the world for the use of its seed oil as a commercial source of biodiesel. Based on 9843 ESTs available from the developing seeds of Jatropha curcas, we identified 1009 SSRs in 4640 unigenes and developed 11 polymorphic EST-SSR markers which exhibited a low level of genetic diversity among germplasms, i.e. allele number varied from 2 to 3, with a average of 2.45; Heterozygosity (He) ranged from 0.0887-0.5128, with a average of 0.2736; Polymorphic Information Content (PIC) ranged from 0.0847-0.4031, with a average of 0.2313. Further, we analyzed the genetic relationships among 24 germplasms collected from different areas in southern China, northern Vietnam, and India using the 11 EST-SSR markers. The results showed that there was no a geographic pattern of genotypes across the collection areas of Jatropha curcas. The EST-SSR markers developed in current study is useful for both genetic diversity analysis and identification of genetic relationships among germplasms in Jatropha curcas.
Key words： Biodiesel plant; EST-SSR; Genetic diversity; Jatropha curcas
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