氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响

Plant Diversity ›› 2008, Vol. 30 ›› Issue (01): 105-109.

氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响

宋经元, 郭顺星, 肖培根

中国医学科学院·中国协和医科大学药用植物研究所, 北京 100094

收稿日期:2007-05-19 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 郭顺星

Effects of Nitrogen Source and Fungal Elicitors on Biomass and Polysaccharide Accumulation in Suspension Cultured Protocorms of Dendrobium candidum (Orchidaceae)

SONG Jing-Yuan, GUO Shun-Xing, XIAO Pei-Gen

Institute of Medicinal Plant Development , Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100094 , China

Received:2007-05-19 Online:2008-02-25 Published:2008-02-25
Contact: GUO Shun-Xing

摘要/Abstract

摘要： 利用正交实验设计研究不同种类和浓度的氮源对铁皮石斛原球茎生长的影响, 利用完全随机实验设计研究不同真菌诱导子对铁皮石斛原球茎生长和多糖积累的影响。结果表明硝态氮促进铁皮石斛原球茎鲜重和干重的增加( P < 0.05); 铵态氮促进铁皮石斛原球茎鲜重增加( P < 0.05) 。无论鲜重还是干重, 硝态氮和铵态氮的影响均不存在互作, 最佳组合为: 67-V 培养液+ 100 mg/L (NH₄ )₂ SO₄ + 800 mg/L KNO₃ + 30g/L 蔗糖+ 200 g/L 马铃薯提取汁。F 检验的结果表明, 14 种真菌诱导子对铁皮石斛原球茎生物量的增加( 鲜重与干重) 均无显著性影响( P > 0.05) 。但与对照铁皮石斛原球茎的多糖含量相比, 真菌诱导子g6、g14、g5、g4 处理可使其多糖含量分别提高14%、9%、6%、4%。本研究获得了有利于铁皮石斛原球茎生长的硝态氮和铵态氮的最佳搭配方案以及有利于铁皮石斛原球茎积累多糖的四种真菌诱导子, 表明通过液体悬浮培养生产铁皮石斛原球茎及其多糖成分具有较好的开发应用前景。

关键词: 正交实验设计, 完全随机实验设计, 铁皮石斛, 生物量, 多糖积累

Abstract: Biotic and abiotic elicitors have dramatically promoted the accumulation of secondary metabolites in plant cell cultures. In this paper , through the orthogonal design and the randomized complete experimental design, we have investigated effects of nitrate source and fungal elicitors on growth and polysaccharide accumulation in suspension cultured protocorms of Dendrobium candidum. It has been found that nitrate (KNO₃) promoted propagation of D. candidum protocorms in suspension culture, fresh weight and dry weight of protocorms increased significantly ( P < 0.05). However, ammonium ( (NH₄)₂ SO₄ ) only stimulated significant increase of fresh weight of protocorms ( P <0. 05), nitrate and ammonium were not interrelated for biomass of protocorms. The results demonstrate that 67-V medium supplemented with (NH₄ )₂ SO₄ 100mg/L, KNO₃ 800mg/L, sucrose 30g/L and potato extract 200 g/L was best for the growth of protocorms. In addition, fourteen fungal elicitors did not significantly influence biomass of suspension cultured protocorms of D. candidum ( P > 0.05). However, compared with the content of polysaccharides in the control, the one in the protocorms of D. candidum elicited by fungi g6, g14, g5 and g4 raised 14%, 9%, 6% and 4% , respectively. Since D. candidum is a rare medicinal plant and polysaccharides are important active components in the plant, our results show good application prospect for producing protocorms of D. candidum in suspension culture.

Key words: Orthogonal design

中图分类号:

Q 945

宋经元, 郭顺星, 肖培根. 氮源和真菌诱导子对铁皮石斛原球茎悬浮培养的影响[J]. Plant Diversity, 2008, 30(01): 105-109.

SONG Jing-Yuan, GUO Shun-Xing, XIAO Pei-Gen. Effects of Nitrogen Source and Fungal Elicitors on Biomass and Polysaccharide Accumulation in Suspension Cultured Protocorms of Dendrobium candidum (Orchidaceae)[J]. Plant Diversity, 2008, 30(01): 105-109.

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