Plant Diversity ›› 2011, Vol. 33 ›› Issue (6): 645-652.DOI: 10.3724/SP.J.1143.2011.11022
郭涛1, 李婉莎2, 查向东1
收稿日期:
2011-02-01
出版日期:
2011-12-25
发布日期:
2011-03-18
基金资助:
国家自然科学基金面上项目(30971452)、中国科学院百人计划项目
GUO Thao-1, LI Wan-Sha-2, ZHA Xiang-Dong-1
Received:
2011-02-01
Online:
2011-12-25
Published:
2011-03-18
Supported by:
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摘要:
以哥伦比亚(Columbia)野生型拟南芥(Arabidopsis thaliana)为实验材料,用含有激活标记双元质粒pCB260的农杆菌浸花进行转化,构建拟南芥TDNA插入突变体库。通过突变体的筛选和表型分析,获得了两株光形态突变体,子叶下胚轴伸长的光抑制效应减弱。通过TAILPCR (thermal asymmetric interlacedPCR)技术,成功扩增出突变植株TDNA插入位点侧翼序列,经NCBI序列比对,TDNA分别插在CRY1第一和第三外显子部位。突变体的表型分析及PCR鉴定结果表明,TDNA插入CRY1并影响到突变植株的光形态建成。
中图分类号:
郭涛1, 李婉莎2, 查向东1. 拟南芥光形态突变体的筛选与基因鉴定[J]. Plant Diversity, 2011, 33(6): 645-652.
GUO Thao-, LI Wan-Sha-, ZHA Xiang-Dong. [J]. Plant Diversity, 2011, 33(6): 645-652.
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