整合生物学期刊网

Plant Diversity ›› 2010, Vol. 32 ›› Issue (S17): 89-96.

• 研究论文 • 上一篇    下一篇

乙烯信号参与调控拟南芥响应PEG模拟的干旱胁迫反应

 杨蕾1, 孙萍2, 林英超2, 唐中华2   

  1. 1 中国科学院上海辰山植物科学研究中心,上海201602;2 东北林业大学森林植物生态学教育部重点实验室,哈尔滨150040
  • 出版日期:2010-12-25 发布日期:2011-01-28
  • 基金资助:

    国家自然科学基金青年科学基金项目(31000176)

Ethylene Signal is Involved in Regulating Arabidopsis Response to Drought Stress Stimulated by PEG

 YANG  Lei-1, SUN  Ping-2, LIN  Ying-Chao-2, TANG  Zhong-Hua-2   

  1. 1 Shanghai Chenshan Plant Science Research Center, Chinese Academy of Sciences, Shanghai 201602, China;
    2 Key Laboratory of Forest Plant Ecology, the Ministry of Education of China, Northeast Forestry University, Harbin 150040, China
  • Online:2010-12-25 Published:2011-01-28
  • Supported by:

    国家自然科学基金青年科学基金项目(31000176)

摘要:

拟南芥ein25、ein31和ctr11是乙烯信号途径中关键基因的突变体,ein25对外源乙烯完全不敏感,ein31为部分不敏感,而ctr11为组成型乙烯反应突变体。乙烯不敏感突变体ein25和ein31在PEG胁迫条件下,其莲座叶的生长、地上生物量、叶面积与Col0和ctr11突变体相比显著地受到抑制,表明乙烯信号对PEG胁迫下拟南芥的形态生长具有积极调控的作用;对各突变体中的叶片水势、相对含水量、MDA含量等各项生理指标进行测定,其结果表明在响应干旱胁迫的反应中,乙烯信号能够调控植物降低水势来维持一定的细胞渗透压,具有更好的保水能力,产生的MDA含量相对较低。乙烯信号在提高植物抗旱性的过程中能够积极地调控渗透调节物质可溶性糖的生成,说明乙烯信号对植物体提高渗透调节能力,抵御干旱胁迫起到了积极的调控作用。

关键词: 乙烯, 信号转导, 干旱胁迫, PEG

Abstract:

Arabidopsis ein25, ein31 and ctr11 are mutants in key genes of ethylene signaling transduction pathway. ein25 is absolutely insensitive to ethylene, ein31 is partly insensitive to ethylene, while ctr11 is constitutive triple response mutant. The morphological observation and measurement showed the rosette growth, shoot biomass and leaf area under PEG stress were restrained more significant in ethylene insensitive mutants ein25 and ein31 than in Col0 and ctr11. From the results, we can conclude that ethylene signal plays a positive role in regulating Arabidopsis growth and morphology under PEG stress. We also determined the physiological status in Arabidopsis under PEG stress. It was indicated that ethylene signal could regulate the plant to reduce the water potential to keep the osmotic potential, resulting in retaining water and enhance the drought tolerance. In the response of ethylene signal to drought, there was more osmotic modulatory substance soluble sugar. And the substance synthesized to a high level in fine ethylene signal pathway plants under PEG stress. We can conclud that ethylene signal can positively mediate the plant to adapt drought stress.

Key words: Ethylene, Signaling transduction, Drought stress, PEG

中图分类号: