高温对萝芙木细胞膜、膜脂和抗坏血酸代谢造成的损伤

Plant Diversity ›› 2008, Vol. 30 ›› Issue (01): 95-98.

高温对萝芙木细胞膜、膜脂和抗坏血酸代谢造成的损伤

田学军¹ , 龙云惠² , 张德刚¹ , 何英²

1 红河学院生物系, 云南蒙自 661100; 2 红河学院化学系, 云南蒙自 661100

收稿日期:2007-04-07 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 田学军

Effect of High Temperature on the Cell Membrane, Membrane Lipid, and Ascorbic Acid Metabolism in Rauwolfia( Rauvolfia vericillata, Apocynaceae)

TIAN Xue-Jun¹, LONG Yun-Hui² , ZHANG De-Gang¹ , HE Ying²

1 Department of Biology , Honghe University , Mengzi 661100 , China ;
2 Department of Chemistry , Honghe University , Mengzi 661100 , China

Received:2007-04-07 Online:2008-02-25 Published:2008-02-25
Contact: TIAN Xue-Jun

摘要/Abstract

摘要： 通过高温胁迫下萝芙木叶片电导率、膜脂过氧化物丙二醛(MDA)、自由基清除剂抗坏血酸的变化研究, 探讨高温对萝芙木组织造成的损伤及自由基清除剂代谢的影响, 从而揭示高温胁迫下萝芙木生长发育迟缓的原因。结果表明, 热胁迫对萝芙木细胞膜造成损伤, 使膜脂更多地被过氧化, 抗坏血酸含量下降, 致使萝芙木受到热损伤和氧化损伤, 最终可能使其生长发育放缓, 开花结果推迟。研究还表明, 萝芙木经适当高温(37℃ ) 热驯, 能产生较多的抗坏血酸, 以清除热胁迫诱导产生的氧自由基, 降低氧化损伤程度。

关键词: 萝芙木, 热胁迫, 电导率, 丙二醛, 抗坏血酸

Abstract: To explore the mechanism of growth retardation under high temperature in Rauwolfia ( Rauvolfia vericillata), the effects of high temperature on membrane integrity, lipid peroxidation , and antioxidant ascorbic acid were examined by measuring the electric conductivity, membrane lipid peroxide malondialdehyde (MDA), and changes of ascorbic acid in Rauwolfia leaves. The results showed that exposure to high temperature (44℃ and 50℃) resulted in increases in electric conductivity and MDA, and decrease in levels of abscorbic acid. Heat acclimation, pretreatment at 37℃, however, maintains ascorbic acid at higher levels than that without heat acclimation , which may be helpful for Rauwolfia to resist heat stress.

Key words: Rauvolfia vericillata

中图分类号:

Q 945

田学军1 , 龙云惠2 , 张德刚1 , 何英2 . 高温对萝芙木细胞膜、膜脂和抗坏血酸代谢造成的损伤[J]. Plant Diversity, 2008, 30(01): 95-98.

TIAN Xue-Jun, LONG Yun-Hui , ZHANG De-Gang , HE Ying. Effect of High Temperature on the Cell Membrane, Membrane Lipid, and Ascorbic Acid Metabolism in Rauwolfia( Rauvolfia vericillata, Apocynaceae)[J]. Plant Diversity, 2008, 30(01): 95-98.

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