黄花杓兰云南中甸居群遗传结构及克隆多样性的分析

Plant Diversity ›› 2008, Vol. 30 ›› Issue (01): 69-75.

黄花杓兰云南中甸居群遗传结构及克隆多样性的分析

蔡凝枫^{1 , 2} , 严宁¹ , 胡虹¹, 刘涛³

1 中国科学院昆明植物研究所, 云南昆明 650204; 2 中国科学院研究生院, 北京 100049;
3 云南农业大学农学与生物技术学院, 云南昆明 650201

收稿日期:2007-04-24 出版日期:2008-02-25 发布日期:2008-02-25
通讯作者: 胡虹

Genetic Structure and Clonal Diversity of Cypripedium flavum (Orchidaceae) Populations from South-West China

CAI Ning-Feng^{1 , 2} , YAN Ning¹, HU Hong¹ , LIU Tao³

1 Kunming Institute of Botany , Chinese Academy of Sciences , Kunming 650204 , China ;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049, China;
3 College of Agronomy and Biotechnology , Yunnan Agricultural University , Kunming 650201 , China

Received:2007-04-24 Online:2008-02-25 Published:2008-02-25
Contact: HU Hong

摘要/Abstract

摘要： 采用AFLP 分子标记方法对云南中甸的6 个黄花杓兰( Cypripedium flavum) 居群进行了遗传多样性水平、遗传结构及克隆多样性研究, 其中两个居群用样方取样法分析其克隆空间结构。POPGENE 软件分析结果表明: 两组引物共产生104 个位点, 其中86 个为多态位点, 多态位点百分率为82.69%。黄花杓兰具有丰富的遗传变异( 物种水平上, H_e = 0.2884, H^_o= 0.4312; 居群水平上, P = 64.59% , H_e = 0.2449 , H_o=0.3606) , 黄花杓兰居群的遗传分化不大( G_st = 0.154) , 居群间基因交流较为频繁( N_m = 2.7460 )。居群的克隆多样性水平高( D= 0.9638 - 0.9960, GN = 0.83 - 0.96) , 同一克隆的分株相邻, 克隆生长延伸范围狭窄。黄花杓兰居群之所以保持较高水平的遗传多样性, 可能与其既能通过实生幼苗增加基因的杂合度, 又能通过无性分株把杂合体固定下来有关。

关键词: 黄花杓兰, 遗传多样性, 遗传结构, 克隆, AFLP

Abstract: Six populations of Cypripedium flavum were studied on their genetic diversity, genetic structure, and clonal diversity in Zhongdian County , Yunnan Province (South-west China). Using POPGENE software, 86 polymorphic loci was obtained using two AFLP primer combinations, and the proportion of polymorphic loci was 82.69%. A relatively high level of genetic diversity was revealed: H_e = 0.2884 , H_o = 0.4312 ( at species level ) ; P = 64.59% , H_e = 0.2449 , Ho = 0.3606 ( at population level ). Genetic differentiation among populations was not high ( G_st = 0.154). A relatively high level of gene flow was obtained among populations. Clonal diversity of populations was high ( D = 0.9638 - 0.9960 , GN = 0.83 - 0.96), and ramets of same genotype were adjacent . C. flavum was found with high level of genetic diversity. This may be explained by gene heterozygosity increasing by seedling recruitment and maintaining by clonal reproduction .

Key words: Cypripedium flavum

中图分类号:

Q 75

蔡凝枫1 , 2 , 严宁1 , 胡虹1 , 刘涛3 . 黄花杓兰云南中甸居群遗传结构及克隆多样性的分析[J]. Plant Diversity, 2008, 30(01): 69-75.

CAI Ning-Feng^, , YAN Ning, HU Hong , LIU Tao. Genetic Structure and Clonal Diversity of Cypripedium flavum (Orchidaceae) Populations from South-West China[J]. Plant Diversity, 2008, 30(01): 69-75.

[1]	Jing Yang, Zerui Gao, Weibang Sun, Changqin Zhang. High regional genetic differentiation of an endangered relict plant Craigia yunnanensis and implications for its conservation[J]. Plant Diversity, 2016, 38(05): 221-226.
[2]	呼慧丽, 李波, 胡菀, 孙志霞, 张志勇, 范邓妹. 南天竹微卫星标记开发及特性分析[J]. Plant Diversity, 2015, 37(2): 141-144.
[3]	余亚莹1, 邵高能1, 圣忠华1, 蒋汉伟2, 贺记外1, 孙园园2, 蔡怡聪1, 胡培松1**, 唐绍清1. 国内外香稻资源遗传多样性研究[J]. Plant Diversity, 2015, 37(06): 871-880.
[4]	徐玉仙, 张微微, 莫海波, 李春, 曹建国, 田代科. 基于ESTSSR标记的莲属种质资源遗传多样性分析[J]. Plant Diversity, 2015, 37(05): 595-604.
[5]	李苗苗, MEEGAHAKUMBURA M. Kasun, 严丽君, 刘杰, 高连明. 核基因组微卫星标记揭示大理茶参与了普洱茶的驯化过程[J]. Plant Diversity, 2015, 37(01): 29-37.
[6]	杜维, 丁勇, 朱东阳, 尹继庭, 刘小烛, 赵平. 樟叶越桔熊果苷合成酶基因VdAS1的克隆及序列分析[J]. Plant Diversity, 2015, 37(01): 71-77.
[7]	于丽霞, 李斌, 黄海泉, 鄢波. 竹亚科lea3基因的进化分析[J]. Plant Diversity, 2014, 36(06): 707-714.
[8]	叶林江, 王静, 孙鹏, 董书鹏, 张志勇. 四种栲属植物微卫星标记在钩栲中的通用性检测[J]. Plant Diversity, 2014, 36(04): 443-448.
[9]	刘博, 沙伟, 张梅娟, 宋璐, 安洪雪. 毛尖紫萼藓GpUCH基因克隆及表达分析[J]. Plant Diversity, 2014, 36(03): 358-364.
[10]	黄家林, 李树云, 胡虹. 珍稀濒危植物硬叶兜兰的遗传多样性及遗传结构研究[J]. Plant Diversity, 2014, 36(02): 209-218.
[11]	吴春燕1、2, 高立志1. 六个核基因片段在中国颗粒野生稻中的单核苷酸多态性及其在群体遗传学研究中的应用[J]. Plant Diversity, 2013, 35(5): 537-546.
[12]	官萌萌1、2, 马瑞1, 龚洵1. 金沙江河谷特有植物罂粟莲花的保护遗传学[J]. Plant Diversity, 2013, 35(5): 555-562.
[13]	赵凯1、2, 王德元2, 张文娟2, 邵剑文2 . 都支杜鹃遗传多样性的ISSR分析[J]. Plant Diversity, 2013, 35(5): 578-584.
[14]	孙晓娇, 汤晓倩, 于丽霞, 白琼, 鄢波. 高粱LEA3蛋白基因和启动子的克隆及序列分析[J]. Plant Diversity, 2013, 35(5): 585-593.
[15]	何庆元1、2, 李正鹏1, 吴萍1, 杨红燕2, 王松华1. 起始密码子区域相关序列多态性的开发及在苜蓿遗传多样性上的应用[J]. Plant Diversity, 2013, 35(1): 48-54.