几种兰花种子无菌萌发及胚胎发育过程的几种途径

Plant Diversity ›› 2006, Vol. 28 ›› Issue (04): 399-402.

几种兰花种子无菌萌发及胚胎发育过程的几种途径

王卜琼; 李枝林; 刘国民; 钱慧生; 余朝秀

云南农业大学

收稿日期:2005-10-25 出版日期:2006-08-25 发布日期:2006-08-25
通讯作者: 李枝林

Study on Seeds Aseptic Culture and Embryo Growth Routes of Several Orchid

ANG Bu-Qiong; LI Zhi-Lin; LIU Guo-Min; QIAN Hui-Sheng; YU Chao-Xiu

Yunnan Agriculture University

Received:2005-10-25 Online:2006-08-25 Published:2006-08-25
Contact: LI Zhi-Lin

摘要/Abstract

摘要： 以虎头兰( 黄色素花) ×大雪兰、大雪兰×虎头兰( 黄色素花) 、虎头兰( 黄色素花) × 黄蝉兰、黄蝉兰×虎头兰( 黄色素花) 、冬凤兰、竹叶兰、窄唇蜘蛛兰、半柱毛兰、三褶虾脊兰和多花脆兰为材料,进行了杂交和自交种子无菌培养研究, 并对胚胎发育进行了显微观察, 结果表明: 兰花种胚的发育途径有两条, 一是种胚突破种皮后再转绿; 二是种胚转绿后再突破种皮, 且两条途径是同时进行的。种胚突破种皮的方式有3 种: 一是种子发育过程中, 种皮慢慢消失, 变成裸露的胚; 二是种胚向种子的一端移动, 最终突破种皮; 三是种胚从种子的一侧突破种皮, 形成裸胚。幼苗的发育途径有两条: 一是小球体经圆球茎发育成小苗; 二是小球体伸长成根茎后发育成小苗。

关键词: 兰花, 种子, 无菌培养, 胚胎学FONT

Abstract: Microexamination were carried out on the embryo growth of the seeds of the orchids of Cymbidium hookerianum× C. mastersii、C. mastersii × C. hookerianum、C. hookerianum × C. iridioides、C. iridioides × C. hookerianum、
C. dayanum、Arundina graminifolia、Arachnis labrosa、Eria corneri、Calanthe triplicate and Acampe multiflora. We have found there were two routes for the embryo growths, carrying through at the same time. One of the route was the embryo become green after breaking through the seed coat, the other was the embryo turned green before breaking through the seed coat. We also discovered that embryo broke through the seed coat through three ways: (1 ) the embryo becomes a bare embryo because the seed coat disappears in growth; (2) the embryo breaks through the seed coat from one aspect of the seed; (3) the embryo breaks through the seed coat from profile, then it becomes a bare embryo. The globules grew into seedlings in two ways: (1) The globules grew into seedlings through their protocorms. (2) The globules grew first into rhizome, then into seedlings.

Key words: Orchid

中图分类号:

Q 945

王卜琼; 李枝林; 刘国民; 钱慧生; 余朝秀. 几种兰花种子无菌萌发及胚胎发育过程的几种途径[J]. Plant Diversity, 2006, 28(04): 399-402.

ANG Bu-Qiong; LI Zhi-Lin; LIU Guo-Min; QIAN Hui-Sheng; YU Chao-Xiu. Study on Seeds Aseptic Culture and Embryo Growth Routes of Several Orchid[J]. Plant Diversity, 2006, 28(04): 399-402.

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