利用ISSR标记对新疆白梭梭居群的遗传多样性分析

Plant Diversity ›› 2006, Vol. 28 ›› Issue (04): 359-362.

利用ISSR标记对新疆白梭梭居群的遗传多样性分析

张萍; 董玉芝; 魏岩; 胡成志

新疆农业大学林学院

收稿日期:2005-10-21 出版日期:2006-08-25 发布日期:2006-08-25
通讯作者: 董玉芝

Analysis of Genetic Diversity of Haloxylon persicum (Chenopodiaceae) in Xinjiang by ISSR

ZHANG Ping; DONG Yu-Zhi; WEI Yan; HU Cheng-zhi

College of Foresty , Xinjiang Agricultural University

Received:2005-10-21 Online:2006-08-25 Published:2006-08-25
Contact: DONG Yu-Zhi

摘要/Abstract

摘要： 利用ISSR 分子标记对新疆白梭梭4 个居群, 105 个个体进行了遗传多样性的比较分析。在供试材料中, 11 个引物共扩增出171 个多态位点, 多态位点百分率为84 . 85% , 4 个居群的多态位点百分率差异在33 . 92%～40 . 35%之间。Shannon 多样性指数( I) 为0 . 3518 , 物种水平的Nei 基因多样度( h) 为0 . 3482。遗传变异分析表明, 物种水平的居群间遗传分化系数Gst 为0 . 6238 , 居群间的基因流Nm 为0 . 3016。遗传分析表明吐鲁番居群和甘家湖居群的遗传距离最近。

关键词: 白梭梭, ISSR, 遗传多样性FONT

Abstract: ISSR molecular markers were used to detect genetic diversity of 105 individuals in four populations of Haloxylon persicum. 171 polymorphic bands were induced by amplication primers. Percentage of polymorphic bands is 84 . 85% and the difference among four populations ranged from 33 . 92% to 40. 35%. Shannon’s indices of diversity (I) is 0. 3518 and Nei’s gene diversity (h) is 0. 3482 respectively. Genetic variance analysis showed that the coefficient of gene differentiation in the species is 0. 6238 and the gene flow is 0.3016 among populations. Genetic distance and cluster analysis indicated that the populations of Ganjiahu and Tulufan had the smallest genetic difference.

Key words: Haloxylon persicum

中图分类号:

Q 943

张萍; 董玉芝; 魏岩; 胡成志. 利用ISSR标记对新疆白梭梭居群的遗传多样性分析[J]. Plant Diversity, 2006, 28(04): 359-362.

ZHANG Ping; DONG Yu-Zhi; WEI Yan; HU Cheng-zhi. Analysis of Genetic Diversity of Haloxylon persicum (Chenopodiaceae) in Xinjiang by ISSR[J]. Plant Diversity, 2006, 28(04): 359-362.

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