关键词: 紫杉烷, 代谢轮廓分析, 红豆杉细胞培养物, 茉莉酸甲酯诱导FONT

Abstract: Taxus chinensis cell cultures were extracted with methanol and then prepared by solid-phase extract prior to HPLC or LC-MS analysis . About 13 taxane compounds were identified or characterized by comparisons with LC-MS and standard referents . The profiling analysis of the taxanes by methyl jasmonate (MJ ) elicitation indicated that the relative chromatographic peak areas of all these identified compounds were increased but the increasing rates differed . Taxchinin M and its structural analogue had the highest increment . The increasing rates of taxol, B -Ⅲ and B -Ⅵ were more than that of taxuyunnanine C and its derivatives, which were oxygenated at C-14 . The results suggested that biosynthsis of taxol and its precursors were more sensitive than oxygenated taxanes at C-14 in response to methyl jasmonate elicitations, while MJ may also elicit the formation of non-effective acetylated taxanes which bear acetate groups at C1 ,C2 , C7 , C9 and C13 . The study may be interesting in metabolic profiling analysis for elucidating taxane biosynthesis .

Key words: Taxanes