野生植物种质资源的保存利用与iFlora

doi:10.3724/SP.J.1143.2012.12149

摘要/Abstract

摘要：

对植物种质资源的保护和可持续利用是人类生存和发展的根基。虽然植物种质资源的收集和保存工作在全球范围内已经取得了阶段性的进展，但植物物种本身复杂的生物学特性、相关信息的不完整性以及收集保存利用过程中专业人才的缺乏，在一定程度上制约了野生植物种质资源的保存和利用。本文以中国野生植物多样性的保护现状为例，探讨了野生植物种质资源收集保存过程中面临的问题，以及iFlora计划的提出和实施对解决这些问题提供的可能解决方案。通过iFlora的实施，将极大地促进和推动我国乃至全球范围内的野生植物多样性保护和利用。作为iFlora的重要元素之一，植物DNA条形码的运用已经逐渐成熟，本文介绍了其在苔藓植物剪叶苔属，以及种子植物榕属、马先蒿属、蒟蒻薯属、红豆杉属和栝楼属中的应用。

关键词: 植物多样性, 植物种质资源, 保存和利用, DNA条形码, iFlora

Abstract:

Plant diversity is the key element of ecosystem, and plays an important role in our daily life and socioeconomic prosperity. Preservation and utilization of plant diversity is the cornerstone of human welfare and civilization, and an essential strategy to address global climate change. Although the plant conservation activities have been achieved significantly in the past decades worldwide, there are gaps existent that may minimize the effort to fulfill the Global Strategy for Plant Conservation (2011-2020), e.g., the complexity of species origin and evolution, the lack of sufficient species information, and the shrunken taxonomy expertise. We account for the plant conservation achievement in China and point part of constrains for the success of China’s strategy for plant conservation, which could be removed with the conceiving and implementation of the nextgeneration Flora, or iFlora project. DNA barcoding is an important element of iFlora. We brief the application of plant DNA barcoding to accurately identify species in the bryophyte genus Herbertus and in a number of genera of seed plants, such as Ficus, Pedicularis, Tacca, Taxus and Trichoshanthes.

Key words: Plant diversity, Plant germplasm, Conservation and utilization, DNA barcoding, iFlora

中图分类号:

杨湘云, 蔡杰, 张挺, 杜燕. 野生植物种质资源的保存利用与iFlora[J]. Plant Diversity, 2012, 34(6): 539-545.

YANG Xiang-Yun, CA Jie, ZHANG Ting, DU Yan. The Potential Contribution of Plant DNA Barcoding and iFlora to Plant Germplasm Conservation[J]. Plant Diversity, 2012, 34(6): 539-545.

参考文献

环境保护部国际合作司主编, 2011. 保护人类赖以生存的生命系统：《生物多样性公约》回顾与展望［M］. 北京: 科学出版社, 88—89
吴征镒, 路安民, 汤彦承等, 2003. 中国被子植物科属综论［M］. 北京: 科学出版社, 1012—1013
吴征镒, 陈心启编, 2004. 中国植物志第1卷［M］. 北京: 科学出版社
中国植物保护战略编委会, 2008. 中国植物保护战略［R］. 广州: 广东科技出版社, 17—18
Bell D, Long DG, Forrest AD et al., 2012. DNA barcoding of European Herbertus (Marchantiopsida, Herbertaceae) and the discovery and description of a new species［J］. Molecular Ecology Resources, 12: 36—47
CBD, 2002. Global Strategy for Plant Conservation, The Secretariat of the Convention on Biological Diversity, Montreal, Canada［M］
CBD, 2010. Updated Global Strategy for Plant Conservation 2011-2020 (http://www.cbd.int/gspc/strategy.shtml)
Cohen JI, Williams JT, Plucknett DL et al., 1991. Ex situ conservation of plant genetic resources: global development and environmental concerns［J］. Science, 253: 866—872
Corvalan C, Hales S, McMichael A, 2005. Millennium Ecosystem Assessment. Ecosystems and Human Wellbeing: Biodiversity Synthesis［M］. Washington DC, USA: World Resources Institute
Engdahl FW, 2007.“Doomsday Seed Vault” in the ArcticBill Gates, Rockefeller and the GMO giants know something we don’t ［Article］. Global Research, December 04, 2007
European Communities, 2008. The Economics of Ecosystems and Biodiversity. An interim Report, European Communities［M］. ISBN13 9789279089602
FAO, IPGRI, 1994. Genebank Standards［R］. Rome: RAO and IPGRI, p. 13, ISBN 9290432365
FAO, 1997. The State of the World’s Plant Genetic Resources for Food and Agriculture［R］, FAO
Farrington B, 2007. Botanical treasure hunt in Botswana ［Newsletter］. Samara, 13: 3—4
Huang H, 2010. Ex situ plant conservation: a key role of Chinese botanic gardens in implementing China’s strategy for plant conservation［J］. BGjournal, 7: 14—19
Kress WJ, Erickson DL, 2007. A twolocus global DNA barcode for land plants: the coding rbcL gene complements the noncoding trnHpsbA spacer region［J］. PLoS One, 2: e508
Kress WJ, Wurdack KJ, Zimmer EA et al., 2005. Use of DNA barcodes to identify flowering plants［J］. Proceedings of the National Academy of Sciences of USA, 102: 8369—8374
Lahaye R, Van der Bank M, Bogarin D et al., 2008. DNA barcoding the floras of biodiversity hotspots［J］. Proceedings of the National Academy of Sciences of USA, 105 (8): 2923—2928
Li DZ, 2008. Floristics and plant biogeography in China［J］. Journal of Integrated. Plant Biology, 50: 771—777
Li DZ (李德铢), Yang XY (杨湘云), Pritchard HW, 2011a. Strategies and challenges in plant germplasm conservation［J］. Plant Diversity and Resources (植物分类与资源学报), 33 (1): 11—18
Li DZ, Gao LM, Li HT et al., 2011b. Comparative analysis of a large dataset indicates that ITS should be incorporated into the core barcode for seed plants［J］. Proceedings of the National Academy of Sciences of USA, 108: 19641—19646
Li DZ (李德铢), Wang YH (王雨华), Yi TS (伊廷双) et al., 2012. The nextgeneration Flora: iFlora［J］. Plant Diversity and Resources (植物分类与资源学报), 34 (6): 525—531
Li HQ, Chen JY, Wang S et al., 2012. Evaluation of six candidate DNA barcoding loci in Ficus (Moraceae) of China［J］. Molecular Ecology Resources, 12: 783—790
Linington SH, Pritchard HW, 2001. Genebanks［A］. In: Levin SA (ed.), Encyclopaedia of Biodiversity, Volume 3［M］. San Francisco: Academic Press, 165—181
Liu J, Mller M, Gao LM et al., 2011. DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species［J］. Molecular Ecology Resources, 11: 89—100
Mittermeier RA, Gil PR, Hoffman M et al., 2005. Hotspots Revisited: Earth’s Biologically Richest and Most Endangered Terrestrial Ecoregions［M］. Chicago: The University of Chicago Press
Myers N, Mittermeier RA, Mittermeier CG et al., 2000. Biodiversity hotspots for conservation priorities［J］. Nature, 403: 853—858
PrescottAllen R, PrescottAllen C, 1990. How many plants feed the world［J］. Conservation Biology, 4: 365—374. (Article first published online: 20 APR 2005 DOI: 10.1111/j.15231739.1990.tb00310.x)
Way M, 2007. Compiling guides for collecting seed from endemic species for the MSBP ［Newsletter］. Samara, 13: 5
Yang HB, Holmgren NH, Mill RR, 1998. Pedicularis Linn［A］. In: Wu ZY, Raven PH (eds.), Flora of China［M］. Beijing: Science Press; St. Louis: Missouri Botanical Garden Press, 16: 97—209
Yu WB (郁文彬), 2011. Species divergence and molecular identification of Pedicularis L. (Orobanchaceae) in the HimalayaHengduan Mountains region. Ph.D. Dissertation［D］. Kunming: Kunming Institute of Botany, Chinese Academy of Sciences
Yu WB, Huang PH, Ree RH et al., 2011. DNA barcoding of Pedicularis L. (Orobanchaceae): Evaluating four universal barcode loci in a large and hemiparasitic genus［J］. Journal of Systematics and Evolution, 49: 425—437
Zhao YM (赵月梅), Zhang L (张玲), 2011. Using DNA barcoding in genus Tacca (Dioscoreaceae) ［J］. Plant Diversity and Resources, 33: 674—682

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