鹤顶兰胚囊发育过程中Ca2+分布的超微细胞化学定位

doi:10.3724/SP.J.1143.2010.10139

Plant Diversity ›› 2010, Vol. 32 ›› Issue (6): 495-502.DOI: 10.3724/SP.J.1143.2010.10139

鹤顶兰胚囊发育过程中Ca2+分布的超微细胞化学定位

李冬妹1,3，王亚琴2，叶秀粦3

1 顺德职业技术学院，广东佛山528300；2 华南师范大学生命科学学院，广东广州510631；
3 中国科学院华南植物园，广东广州510650

收稿日期:2010-07-29 出版日期:2010-12-25 发布日期:2010-10-09
基金资助:
Plan Project sponsored by the Department of Science and Technology of Guangdong Province (2009B020405002)

Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)

LI Dong-Mei-1,3, WANG Y-Qin-2, YIE Xiu-Lin-3

1 Shunde Polytechnic, Foshan 528300, China; 2 College of Life Science, South China
Normal University, Guangzhou 510631, China; 3 South China Botanical Garden,
Chinese Academy of Sciences, Guangzhou 510650, China

Received:2010-07-29 Online:2010-12-25 Published:2010-10-09
Supported by:
Plan Project sponsored by the Department of Science and Technology of Guangdong Province (2009B020405002)

摘要/Abstract

摘要：

用焦锑酸盐沉淀法对鹤顶兰（Phaius tankervilliae）胚囊发育过程中的Ca2+状态进行超微细胞化学定位。观察结果发现：功能大孢子时期，珠孔端的胚囊壁上开始出现小颗粒的Ca2+沉淀，但功能大孢子细胞内未见明显的Ca2+标记；四核胚囊时期胚囊壁上的Ca2+沉淀明显增多，液泡膜上有Ca2+沉淀出现，珠孔处的Ca2+沉淀颗粒较大；成熟胚囊时期，胚囊壁上的Ca2+沉淀进一步增多，且胚囊内Ca2+分布明显增多，且极性明显，珠孔端助细胞、卵细胞比合点端反足细胞有更多的Ca2+沉淀。鹤顶兰成熟胚囊内Ca2+积累的来源有：（1）在胚囊成熟前主要由珠被细胞、珠细胞通过胞间连丝向胚囊运输；（2）以沉淀有大量Ca2+的小泡形式跨过胚囊壁进入胚囊。

关键词: 鹤顶兰, 胚囊, 超微细胞化学定位, 焦锑酸盐沉淀法, 钙

Abstract:

Ultracytochemical localization of calcium in embryo sac of Phaius tankervilliae(Aiton) Bl. was carried out using potassium pyroantimonate precipitation method. Results of Ca2+ distribution during embryo sac development are as following: (1) Megasporocyte stage: There is no Ca2+ precipitation in the megasporocyte, nucellar cell and micropyle; (2) Functional megaspore stage: Small particles of Ca2+ precipitates appear on the embryo sac wall at the micropylar end, but no apparent Ca2+ can be found in the functional megaspore; (3) 4nucleate stage: There are significant increase of Ca2+ precipitation on the embryo sac wall and large grains of the precipitates in the micropyle, while a little starts to appear on the membrane of vacuole; (4) 8nucleate embryo sac stage: Ca2+ precipitation continuously increases on the embryo sac wall. Distribution of Ca2+ in embryo sac shows strong polarity. More Ca2+ precipitations are observed in the synergids and the egg cell than the antipodal cells. Accumulation of Ca2+ precipitation is achieved by (1) transferring Ca2+ to embryo sac through the plasmodesmata between the nucellar cells, which mainly occurred at the chalaza end before the embryo matured; (2) forming large amounts of small bubbles of Ca2+ precipitation, which can cross embryo sac wall to enter embryo sac.

Key words: Phaius tankervilliae, Embryo sac, Ultracytochemistry, Potassium antimonite technique, Ca2+

中图分类号:

Q 944

李冬妹1,3, 王亚琴2, 叶秀粦3. 鹤顶兰胚囊发育过程中Ca2+分布的超微细胞化学定位[J]. Plant Diversity, 2010, 32(6): 495-502.

LI Dong-Mei-, WANG Y-Qin-, YIE Xiu-Lin. Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)[J]. Plant Diversity, 2010, 32(6): 495-502.

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鹤顶兰胚囊发育过程中Ca2+分布的超微细胞化学定位

Ultracytochemical Localization of Calcium during Embryo Sac Development in Phaius tankervilliae(Orchidaceae)

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