Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)

doi:10.1016/j.pld.2025.06.009

Plant Diversity ›› 2026, Vol. 48 ›› Issue (01): 107-116.DOI: 10.1016/j.pld.2025.06.009

Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)

Terezie Mandáková^a,b, Milan Pouch^a,c, Petra Hloušková^a, Dmitry A. German^d, Pavel Trávníček^e, Michael D. Windham^f, Martin A. Lysak^a,b

a Central European Institute of Technology, Masaryk University, Brno, Czech Republic;
b Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic;
c National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic;
d South-Siberian Botanical Garden, Altai State University, Barnaul, Russia;
e Institute of Botany, Czech Academy of Sciences, Průhonice, Czech Republic;
f Department of Biology, Duke University, Durham, USA

收稿日期:2025-03-20 修回日期:2025-06-18 出版日期:2026-01-25 发布日期:2026-03-05
通讯作者: Terezie Mandáková,E-mail:terezie.mandakova@ceitec.muni.cz;Milan Pouch,E-mail:milan.pouch@ceitec.muni.cz;Petra Hloušková,E-mail:petra.hlouskova@ceitec.muni.cz;Dmitry A. German,E-mail:oreoloma@rambler.ru;Pavel Trávníček,E-mail:Pavel.Travnicek@ibot.cas.cz;Michael D. Windham,E-mail:michael.windham@duke.edu;Martin A. Lysak,E-mail:martin.lysak@ceitec.muni.cz
基金资助:
This work was supported by the Czech Science Foundation (projects 24-11371S to TM and 23- 06840S to MAL), and Masaryk University Grant Agency (project MUNI/R/1268/2022 to TM). Additional support (PT) was provided as part of a long-term research project of the Czech Academy of Sciences, Institute of Botany (RVO 67985939). Core Facility Genomics of CEITEC Masaryk University is gratefully acknowledged for the obtaining of the scientific data presented in this paper. Computational resources for RepeatExplorer analysis were provided by the ELIXIR-CZ project (LM2023055), part of the international ELIXIR infrastructure.

Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)

Terezie Mandáková^a,b, Milan Pouch^a,c, Petra Hloušková^a, Dmitry A. German^d, Pavel Trávníček^e, Michael D. Windham^f, Martin A. Lysak^a,b

a Central European Institute of Technology, Masaryk University, Brno, Czech Republic;
b Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czech Republic;
c National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czech Republic;
d South-Siberian Botanical Garden, Altai State University, Barnaul, Russia;
e Institute of Botany, Czech Academy of Sciences, Průhonice, Czech Republic;
f Department of Biology, Duke University, Durham, USA

Received:2025-03-20 Revised:2025-06-18 Online:2026-01-25 Published:2026-03-05
Contact: Terezie Mandáková,E-mail:terezie.mandakova@ceitec.muni.cz;Milan Pouch,E-mail:milan.pouch@ceitec.muni.cz;Petra Hloušková,E-mail:petra.hlouskova@ceitec.muni.cz;Dmitry A. German,E-mail:oreoloma@rambler.ru;Pavel Trávníček,E-mail:Pavel.Travnicek@ibot.cas.cz;Michael D. Windham,E-mail:michael.windham@duke.edu;Martin A. Lysak,E-mail:martin.lysak@ceitec.muni.cz
Supported by:
This work was supported by the Czech Science Foundation (projects 24-11371S to TM and 23- 06840S to MAL), and Masaryk University Grant Agency (project MUNI/R/1268/2022 to TM). Additional support (PT) was provided as part of a long-term research project of the Czech Academy of Sciences, Institute of Botany (RVO 67985939). Core Facility Genomics of CEITEC Masaryk University is gratefully acknowledged for the obtaining of the scientific data presented in this paper. Computational resources for RepeatExplorer analysis were provided by the ELIXIR-CZ project (LM2023055), part of the international ELIXIR infrastructure.

摘要/Abstract

摘要： The genus Boechera (Brassicaceae) serves as a model system for studying apomictic reproduction and ecological adaptations, with most species occurring in North America. The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments. One such species, B. calcarea, has been described from the Chandalaz Mountains in northeastern Asia (Russia). This study aimed to investigate the genome structure and evolutionary history of B. calcarea. However, our analyses reveal that the species does not belong to Boechera. Instead, an integrative approach combining cytogenetic, phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae. The diploid Parryodes calcarea (2n = 16) exhibits Arabideae-specific chromosomal signatures, including multiple centromere repositionings. These findings clarify the misclassification of P. calcarea as Boechera, leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World. This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe, the Arabideae.

关键词: Arabideae, Cruciferae, Chromosome structure, Centromere repositioning, Cytogenetics, Phylogenetic reconstruction

Abstract: The genus Boechera (Brassicaceae) serves as a model system for studying apomictic reproduction and ecological adaptations, with most species occurring in North America. The rare occurrence of Boechera species outside their typical range provides unique opportunities to investigate genome evolution in extralimital environments. One such species, B. calcarea, has been described from the Chandalaz Mountains in northeastern Asia (Russia). This study aimed to investigate the genome structure and evolutionary history of B. calcarea. However, our analyses reveal that the species does not belong to Boechera. Instead, an integrative approach combining cytogenetic, phylogenetic and repeatome analysis identified the species as a member of one of more ancestral clades in the tribe Arabideae. The diploid Parryodes calcarea (2n = 16) exhibits Arabideae-specific chromosomal signatures, including multiple centromere repositionings. These findings clarify the misclassification of P. calcarea as Boechera, leaving Boechera falcata and Borodinia macrophylla as the only representatives of the Boechereae in the Old World. This study highlights the importance of an integrative approach in resolving taxonomic ambiguities and provides new insights into the diversification of the largest cruciferous tribe, the Arabideae.

Key words: Arabideae, Cruciferae, Chromosome structure, Centromere repositioning, Cytogenetics, Phylogenetic reconstruction

Terezie Mandáková, Milan Pouch, Petra Hloušková, Dmitry A. German, Pavel Trávníček, Michael D. Windham, Martin A. Lysak. Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)[J]. Plant Diversity, 2026, 48(01): 107-116.

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Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)

Boechera or not? Genomic insights and taxonomic reassessment of the misclassified Asian species B. calcarea (Brassicaceae)

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