Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities

doi:10.1007/s13659-023-00393-0

Natural Products and Bioprospecting ›› 2023, Vol. 13 ›› Issue (4): 26-26.DOI: 10.1007/s13659-023-00393-0

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Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities

Shu-Hui Wang, Cong Guo, Wen-Jin Cui, Qing-Xia Xu, Jun Zhang, Jin-Zhu Jiang, Yan Liu, Sha Chen, Chang Chen, Jin-Tang Cheng, An Liu

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

Received:2023-06-22 Online:2023-10-08 Published:2023-08-24
Contact: Jin-Tang Cheng,E-mail:jtcheng@icmm.ac.cn;An Liu,E-mail:la62@163.com
Supported by:
We gratefully acknowledge financial support from Scientific and technological innovation project of China Academy of Chinese Medical Sciences (CI2021A04409, CI2021A04404, CI2021A04405), the fundamental research funds for the central public welfare research institutes (No. ZZ13-YQ-061, ZXKT22012, ZXKT22039).

Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities

Shu-Hui Wang, Cong Guo, Wen-Jin Cui, Qing-Xia Xu, Jun Zhang, Jin-Zhu Jiang, Yan Liu, Sha Chen, Chang Chen, Jin-Tang Cheng, An Liu

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, China

通讯作者: Jin-Tang Cheng,E-mail:jtcheng@icmm.ac.cn;An Liu,E-mail:la62@163.com
基金资助:
We gratefully acknowledge financial support from Scientific and technological innovation project of China Academy of Chinese Medical Sciences (CI2021A04409, CI2021A04404, CI2021A04405), the fundamental research funds for the central public welfare research institutes (No. ZZ13-YQ-061, ZXKT22012, ZXKT22039).

Abstract

Abstract: An undescribed pyrrole acid, 1-(4'-methoxy-4'-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid (1) and one known pyrrole acid (2) were isolated from the fruits of Phyllanthus emblica. The structures of these compounds were elucidated via the comprehensive analyses of IR, HRESIMS, 1D and 2D spectroscopic data. A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide (NO), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor-α (TNF-α) by reducing the phosphorylation of extracellular regulated protein kinases (ERK) and c-Jun N-terminal kinases (JNK) in RAW 264.7 cells. Additionally, compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.

Key words: Pyrrole acid, Phyllanthus emblica L., Lipid deposition, ATP-binding cassette transporter A1, RAW264.7 macrophages

摘要： An undescribed pyrrole acid, 1-(4'-methoxy-4'-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid (1) and one known pyrrole acid (2) were isolated from the fruits of Phyllanthus emblica. The structures of these compounds were elucidated via the comprehensive analyses of IR, HRESIMS, 1D and 2D spectroscopic data. A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide (NO), interleukin-6 (IL-6), monocyte chemotactic protein 1 (MCP-1) and tumor necrosis factor-α (TNF-α) by reducing the phosphorylation of extracellular regulated protein kinases (ERK) and c-Jun N-terminal kinases (JNK) in RAW 264.7 cells. Additionally, compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.

关键词: Lipid deposition, ATP-binding cassette transporter A1, RAW264.7 macrophages, Pyrrole acid, Phyllanthus emblica L.

Shu-Hui Wang, Cong Guo, Wen-Jin Cui, Qing-Xia Xu, Jun Zhang, Jin-Zhu Jiang, Yan Liu, Sha Chen, Chang Chen, Jin-Tang Cheng, An Liu. Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities[J]. Natural Products and Bioprospecting, 2023, 13(4): 26-26.

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Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities

Two pyrrole acids isolated from Phyllanthus emblica L. and their bioactivities

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