Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

doi:10.1007/s13659-012-0017-0

Natural Products and Bioprospecting ›› 2012, Vol. 2 ›› Issue (2): 87-91.DOI: 10.1007/s13659-012-0017-0

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

Ramona LIEDER^a,b, Sigrídur Thóra REYNISDÓTTIR^a, Finnbogi THORMÓDSSON^e, Chuen-How NG^c, Jon Magnús EINARSSON^c, Jóhannes GÍSLASON^c, Jóhannes BJÖRNSSON^d,f, Sveinn GUDMUNDSSON^a, Pétur Henry PETERSEN^e, Ólafur Eysteinn SIGURJÓNSSON^a,b,d

a The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland;
b School of Science and Engineering, Reykjavik University, Menntavegi 1, 101 Reykjavik, Iceland;
c Genis ehf, Vatnagördum 18, 104 Reykjavik, Iceland;
d Biomedical Center, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
e Department of Anatomy, Medical Faculty, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
f Department of Pathology, Landspitali University Hospital, Baronsstig, 101 Reykjavik, Iceland

Received:2012-02-28 Revised:2012-03-25 Online:2018-02-11 Published:2012-04-24
Supported by:
This work was supported by grants from Technology Development Fund, managed by the Icelandic Center for Research, and Landspitali University Hospital Research Fund.

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

a The Blood Bank, Landspitali University Hospital, Snorrabraut 60, 105 Reykjavik, Iceland;
b School of Science and Engineering, Reykjavik University, Menntavegi 1, 101 Reykjavik, Iceland;
c Genis ehf, Vatnagördum 18, 104 Reykjavik, Iceland;
d Biomedical Center, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
e Department of Anatomy, Medical Faculty, University of Iceland, Vatnsmyrarvegi 16, 101 Reykjavik, Iceland;
f Department of Pathology, Landspitali University Hospital, Baronsstig, 101 Reykjavik, Iceland

通讯作者: Ólafur Eysteinn SIGURJÓNSSON,E-mail:oes@landspitali.is
基金资助:
This work was supported by grants from Technology Development Fund, managed by the Icelandic Center for Research, and Landspitali University Hospital Research Fund.

Abstract

Abstract: Human mesenchymal stem cells(hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins(CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

Key words: YKL-40, mesenchymal stem cells, osteogenic differentiation, chitinase-like-protein

摘要： Human mesenchymal stem cells(hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins(CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes.

关键词: YKL-40, mesenchymal stem cells, osteogenic differentiation, chitinase-like-protein

Ramona LIEDER, Sigrídur Thóra REYNISDÓTTIR, Finnbogi THORMÓDSSON, Chuen-How NG, Jon Magnús EINARSSON, Jóhannes GÍSLASON, Jóhannes BJÖRNSSON, Sveinn GUDMUNDSSON, Pétur Henry PETERSEN, Ólafur Eysteinn SIGURJÓNSSON. Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation[J]. Natural Products and Bioprospecting, 2012, 2(2): 87-91.

References

[1] Sakaguchi, Y.; Sekiya, I.; Yagishita, K.; Muneta, T. Arthritis Rheum. 2005, 52, 2521-2529.
[2] Zuk, P. A.; Zhu, M.; Mizuno, H.; Huang, J.; Futrell, J. W.; Katz, A. J.; Benhaim, P.; Lorenz, H. P.; Hedrick, M. H. Tissue Eng. 2001, 7, 211-228.
[3] Jiang, Y.; Jahagirdar, B. N.; Reinhardt, R. L.; Schwartz, R. E.; Keene, C. D.; Ortiz-Gonzalez, X. R.; Reyes, M.; Lenvik, T.; Lund, T.:Blackstad, M.; Du, J.; Aldrich, S.; Lisberg, A.; Low, W. C.; Largaespada, D. A.; Verfaillie, C. M. Nature 2002, 418, 41-49.
[4] Bleau, G.; Massicotte, F.; Merlen, Y.; Boisvert, C. EXS 1999, 87, 211-221.
[5] Lee, C. G. Yonsei Med. J. 2009, 50, 22-30.
[6] Recklies, A. D.; Ling. H.; White, C.; Bernier, S. M. J. Biol. Chem. 2005, 280, 41213-41221.
[7] Bussink, A. P.; Speijer, D.; Aerts, J. M.; Boot, R. G. Genetics 2007, 177, 959-970.
[8] Renkema, G. H.; Boot, R. G.; Au, F. L.; Donker-Koopman, W. E.; Strijland, A.; Muijsers, A. O.; Hrebicek, M.; Aerts, J. M. Eur. J. Biochem. 1998, 251, 504-509.
[9] Holt, P. G. Toxicol. Lett. 1996, 86, 205-210.
[10] Houston, D. R.; Recklies, A. D.; Krupa, J. C.; van Aalten, D. M. J. Biol. Chem. 2003, 278, 30206-30212.
[11] Hakala, B. E.; White, C.; Recklies, A. D. J. Biol. Chem. 1993, 268, 25803-25810.
[12] Ling, H.; Recklies, A. D. Biochem. J. 2004, 380, 651-659.
[13] Sandford, P. A. Advances in Chitin Science; NTNU:Trondheim, 2003; pp. 35-42.
[14] Kirkham, S. G.; Samarasinghe, R. K. J. Orthop. Surg. (Hong Kong) 2009, 17, 72-76.
[15] Meininger, C. J.; Kelly, K. A.; Li, H.; Haynes, T. E.; Wu, G. Biochem. Biophys. Res. Commun. 2000, 279, 234-239.
[16] Ivanovska, N.; Dimitrova, P. Arthritis Res. Ther. 2011, 13, R44.
[17] Dominici, M.; Le Blanc, K.; Mueller, I.; Slaper-Cortenbach, I.; Marini, F.; Krause, D.; Deans, R.; Keating, A.; Prockop, D.; Horwitz, E. Cytotherapy 2006, 8, 315-317.
[18] Bergkvist, A.; Rusnakova, V.; Sindelka, R.; Garda, J. M.; Sjogreen, B.; Lindh, D.; Forootan, A.; Kubista, M. Methods 2010, 50, 323-335.
[19] Disthabanchong, S.; Radinahamed, P.; Stitchantrakul, W.; Hongeng, S.; Rajatanavin, R. Kidney Int. 2007, 71, 201-209.
[20] Ganno, T.; Yamada, S.; Ohara, N.; Matsunaga, T.; Yanagiguchi, K.; Ikeda, T.; Ishizaki, H.; Hayashi, Y. J. Biomed. Mater. Res. A 2007, 82, 188-194.

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 1

Recommended Articles

Metrics

Comments