A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro

doi:10.1007/s13659-025-00542-7

应用天然产物 ›› 2025, Vol. 15 ›› Issue (6): 60-60.DOI: 10.1007/s13659-025-00542-7

• Original Article • 上一篇下一篇

A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro

Xiao Han¹, Xin-Xiu Ren^1,2, Dan-Yang Zhang¹, Qin-Feng Guo¹, Shi-Meng Li¹, Zhi-Long Xiu¹, Yue-Sheng Dong¹

1. MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, 116024, Liaoning, China;
2. College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, China

收稿日期:2025-04-02 出版日期:2025-12-23 发布日期:2026-01-12
通讯作者: Yue-Sheng Dong Email:E-mail:yshdong@dlut.edu.cn
基金资助:
This research was funded by The Yunnan Talent Support Plan for Industrial Innovation Talents, Major Science and Technology Projects in Yunnan Province (202202AE090075), The Academician (Expert) Workstation Construction Project of Yunnan Province (202205AF15007). We acknowledge the assistance of DUT Core Facilities of School of Bioengineering.

A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro

Xiao Han¹, Xin-Xiu Ren^1,2, Dan-Yang Zhang¹, Qin-Feng Guo¹, Shi-Meng Li¹, Zhi-Long Xiu¹, Yue-Sheng Dong¹

1. MOE Key Laboratory of Bio-Intelligent Manufacturing, School of Bioengineering, Dalian University of Technology, Dalian, 116024, Liaoning, China;
2. College of Basic Medical Sciences, Dalian Medical University, Dalian, 116044, China

Received:2025-04-02 Online:2025-12-23 Published:2026-01-12
Contact: Yue-Sheng Dong Email:E-mail:yshdong@dlut.edu.cn
Supported by:
This research was funded by The Yunnan Talent Support Plan for Industrial Innovation Talents, Major Science and Technology Projects in Yunnan Province (202202AE090075), The Academician (Expert) Workstation Construction Project of Yunnan Province (202205AF15007). We acknowledge the assistance of DUT Core Facilities of School of Bioengineering.

摘要/Abstract

摘要： Polysaccharides are the primary active constituents of Polygonatum kingianum Coll. et Hemsl. However, the comprehensive characterization of P. kingianum polysaccharides (PKP) remains scarce, impeding investigations into the structure-activity relationship. In this study, a novel polysaccharide, PKP1, was purified using Cellulose DE-52 and Sephadex G-50 column chromatography, and its complete structure was elucidated through monosaccharide composition analysis, methylation analysis, as well as 1D and 2D NMR analysis. The results revealed that PKP1 primarily comprised Fru and Glc, exhibiting a molecular weight of 5.3 × 10³ Da and a polymer dispersity index of 1.20. The completed structure of PKP1 consisted of β-D-Fruf-(2 → , → 1,2)-β-D-Fruf-(6 → , → 1)-β-D-Fruf-(2 → and → 1)-α-D-Glcp-(6 → as the main chain sugar residues, with β-D-Fruf-(2 → and → 2)-β-D-Fruf-(6 → serving as the side chains sugar residues. The detailed structure of PKP1 suggested it is a novel Fru-dominated neutral polysaccharide. Biological assays indicated that PKP1 significantly reduced the levels of NO, IL-6, and TNF-α in RAW264.7 macrophages, while also exerting regulatory effects on the gut microbiota structure and its metabolites in vitro. Our findings enriched the understanding of the structural characteristics of P. kingianum polysaccharides and laid a solid foundation for considering P. kingianum as a potential functional food supplement.

关键词: Polygonatum kingianum Coll. et Hemsl., Polysaccharide, Structure elucidation, Bioactivity

Abstract: Polysaccharides are the primary active constituents of Polygonatum kingianum Coll. et Hemsl. However, the comprehensive characterization of P. kingianum polysaccharides (PKP) remains scarce, impeding investigations into the structure-activity relationship. In this study, a novel polysaccharide, PKP1, was purified using Cellulose DE-52 and Sephadex G-50 column chromatography, and its complete structure was elucidated through monosaccharide composition analysis, methylation analysis, as well as 1D and 2D NMR analysis. The results revealed that PKP1 primarily comprised Fru and Glc, exhibiting a molecular weight of 5.3 × 10³ Da and a polymer dispersity index of 1.20. The completed structure of PKP1 consisted of β-D-Fruf-(2 → , → 1,2)-β-D-Fruf-(6 → , → 1)-β-D-Fruf-(2 → and → 1)-α-D-Glcp-(6 → as the main chain sugar residues, with β-D-Fruf-(2 → and → 2)-β-D-Fruf-(6 → serving as the side chains sugar residues. The detailed structure of PKP1 suggested it is a novel Fru-dominated neutral polysaccharide. Biological assays indicated that PKP1 significantly reduced the levels of NO, IL-6, and TNF-α in RAW264.7 macrophages, while also exerting regulatory effects on the gut microbiota structure and its metabolites in vitro. Our findings enriched the understanding of the structural characteristics of P. kingianum polysaccharides and laid a solid foundation for considering P. kingianum as a potential functional food supplement.

Key words: Polygonatum kingianum Coll. et Hemsl., Polysaccharide, Structure elucidation, Bioactivity

Xiao Han, Xin-Xiu Ren, Dan-Yang Zhang, Qin-Feng Guo, Shi-Meng Li, Zhi-Long Xiu, Yue-Sheng Dong. A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro[J]. 应用天然产物, 2025, 15(6): 60-60.

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A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro

A novel polysaccharide in Polygonatum kingianum: structure elucidation, the activities of anti-inflammatory and the regulation of gut microbiota in vitro

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