Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

doi:10.1007/s13659-024-00476-6

应用天然产物 ›› 2024, Vol. 14 ›› Issue (6): 52-52.DOI: 10.1007/s13659-024-00476-6

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Ni Huang^1,2, Yi-Na Yang^1,3, Jia Huang¹, Hui-Yan Shao¹, Yan-Lang Li¹, Shi-Hui Qin^1,3, Han-Fen Li^1,2, Xiao-Jiang Shen¹, Liu Yang¹, Jiang-Miao Hu¹

1. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing, 100049, China;
3. College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, Anhui, China

收稿日期:2024-05-27 出版日期:2024-12-24 发布日期:2024-12-13
通讯作者: Liu Yang,E-mail:yangliu@mail.kib.ac.cn;Jiang-Miao Hu,E-mail:hujiangmiao@mail.kib.ac.cn
基金资助:
Key Project of Yunnan Provincial Nature Foundation (202401AS070075), the authors declare no competing interests.

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Ni Huang^1,2, Yi-Na Yang^1,3, Jia Huang¹, Hui-Yan Shao¹, Yan-Lang Li¹, Shi-Hui Qin^1,3, Han-Fen Li^1,2, Xiao-Jiang Shen¹, Liu Yang¹, Jiang-Miao Hu¹

1. Key Laboratory of Phytochemistry and Natural Medicines, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, Yunnan, China;
2. University of Chinese Academy of Sciences, Beijing, 100049, China;
3. College of Pharmacy, Anhui University of Chinese Medicine, Hefei 230012, Anhui, China

Received:2024-05-27 Online:2024-12-24 Published:2024-12-13
Contact: Liu Yang,E-mail:yangliu@mail.kib.ac.cn;Jiang-Miao Hu,E-mail:hujiangmiao@mail.kib.ac.cn
Supported by:
Key Project of Yunnan Provincial Nature Foundation (202401AS070075), the authors declare no competing interests.

摘要/Abstract

摘要： Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10³ and 1.25 × 10⁴ Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.

关键词: Dictyophora rubrovalvata, Polysaccharides, Structure characterization, Immunoactivity

Abstract: Dictyophora rubrovalvata is a valuable fungus homologous to food and medicine, and its polysaccharide have been gaining increasing attention because of its plentiful activity. However, the structure and activity of its homogeneous polysaccharide have not been studied enough. In this study, two polysaccharides DRP-I and DRP-II were purified from D. rubrovalvata. Their structures were characterized by chemical composition, monosaccharide composition analysis, methylation analysis and nuclear magnetic resonance spectroscopy. The results showed that DRP-I and DRP-II were neutral heteropolysaccharides with molecular weights of 5.79 × 10³ and 1.25 × 10⁴ Da, respectively, which were composed of mannose, galactose, glucose, xylose and fucose. The main chains were → 6)-α-D-Galp-(1 → 6)-α-D-Galp-(2,1 → 6)-α-D-Manp-(2,1 → 6)-α-D-Galp-(1, and branch chains were β-D-Xylp-(1 → 3)-α-L-Fucp-(1 → 4)-α-D-Manp-(1 → and α-D-Galp-(1 → 3)-α-D-Galp-(1 → . The in vitro immunoactivity assays on dendritic cells showed that DRP-I and DRP-II could up-regulate the expression of IL-10 and IL-6 and inhibit the expression of TNF-α in a concentration-dependent manner. This research indicated that DRP-I and DRP-II possessed immunoactivity by balancing the excessive inflammation, and molecular weight is an important factor affecting immunoactivity.

Key words: Dictyophora rubrovalvata, Polysaccharides, Structure characterization, Immunoactivity

Ni Huang, Yi-Na Yang, Jia Huang, Hui-Yan Shao, Yan-Lang Li, Shi-Hui Qin, Han-Fen Li, Xiao-Jiang Shen, Liu Yang, Jiang-Miao Hu. Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata[J]. 应用天然产物, 2024, 14(6): 52-52.

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Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

Structure characterization and immunoactivity on dendritic cells of two neutral polysaccharides from Dictyophora rubrovalvata

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