Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine

doi:10.1007/s13659-023-00389-w

应用天然产物 ›› 2023, Vol. 13 ›› Issue (4): 25-25.DOI: 10.1007/s13659-023-00389-w

• ORIGINAL ARTICLES • 上一篇下一篇

Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine

Yun Zhao^1,3, Qiong Jin^1,3, Zi-Jiao Wang^1,3, Xing-Yu Tao^1,3, Xiao-Dong Luo^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China;
2. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
3. University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China

收稿日期:2023-07-03 出版日期:2023-08-24 发布日期:2023-10-08
通讯作者: Xiao-Dong Luo,E-mail:xdluo@mail.kib.ac.cn
基金资助:
The authors gratefully acknowledge the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006) financial support. We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support. We are grateful to the State Key Laboratory for Conservation and Utilization of Bio-Resources in Kunming, Yunnan Province, P. R. China for providing fungal cultures.

Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine

Yun Zhao^1,3, Qiong Jin^1,3, Zi-Jiao Wang^1,3, Xing-Yu Tao^1,3, Xiao-Dong Luo^1,2

1. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650201, People's Republic of China;
2. Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, Yunnan Characteristic Plant Extraction Laboratory, School of Chemical Science and Technology, Yunnan University, Kunming, 650500, People's Republic of China;
3. University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China

Received:2023-07-03 Online:2023-08-24 Published:2023-10-08
Contact: Xiao-Dong Luo,E-mail:xdluo@mail.kib.ac.cn
Supported by:
The authors gratefully acknowledge the High-level Talent Promotion and Training Project of Kunming (2022SCP003), Project of Yunnan Characteristic Plant Screening and R&D Service CXO Platform (2022YKZY001), and Scientific and Technological Innovation Team of Yunnan Province (202105AE160006) financial support. We thank the Advanced Analysis and Measurement Center of Yunnan University for technical support. We are grateful to the State Key Laboratory for Conservation and Utilization of Bio-Resources in Kunming, Yunnan Province, P. R. China for providing fungal cultures.

摘要/Abstract

摘要： Worldwide, fruit is an indispensable treasure house of nutrition for human beings, occupying a vital position of human diet. Postharvest fruit storage requires efficient antifungal agents to control Botrytis cinerea, which is a vital postharvest disease affecting fruit and leading to enormous losses. However, with the enormous abuse of existing antifungal drugs, the problem of drug-resistant fungi is imminent, making the controlling diseases caused by pathogenic fungi even more challenging. Drug repurposing is an efficient alternative method, we evaluated a well-known antifungal chemical, terbinafine, against the agricultural pathogen, B. cinerea in vitro, as a result, terbinafine showed strong antifungal activity. Furthermore, the in vivo antifungal activity of terbinafine was evaluated, the results showed that terbinafine could reduce the decay area on grapes. Terbinafine could disrupt the cell membrane integrity, increase cell membrane permeability, and eventual cell death of B. cinerea. In addition, terbinafine reduced decay incidence, and weight loss and maintained the soluble solids, titratable acidity, ascorbic acid, total phenolic, and malondialdehyde content during the storage period of grapes. Overall, terbinafine could be an antifungal preservative for postharvest table grapes fresh-keeping.

关键词: Terbinafine, Botrytis cinerea, Antifungal activity, Cell membrane, Grape preservation

Abstract: Worldwide, fruit is an indispensable treasure house of nutrition for human beings, occupying a vital position of human diet. Postharvest fruit storage requires efficient antifungal agents to control Botrytis cinerea, which is a vital postharvest disease affecting fruit and leading to enormous losses. However, with the enormous abuse of existing antifungal drugs, the problem of drug-resistant fungi is imminent, making the controlling diseases caused by pathogenic fungi even more challenging. Drug repurposing is an efficient alternative method, we evaluated a well-known antifungal chemical, terbinafine, against the agricultural pathogen, B. cinerea in vitro, as a result, terbinafine showed strong antifungal activity. Furthermore, the in vivo antifungal activity of terbinafine was evaluated, the results showed that terbinafine could reduce the decay area on grapes. Terbinafine could disrupt the cell membrane integrity, increase cell membrane permeability, and eventual cell death of B. cinerea. In addition, terbinafine reduced decay incidence, and weight loss and maintained the soluble solids, titratable acidity, ascorbic acid, total phenolic, and malondialdehyde content during the storage period of grapes. Overall, terbinafine could be an antifungal preservative for postharvest table grapes fresh-keeping.

Key words: Terbinafine, Botrytis cinerea, Antifungal activity, Cell membrane, Grape preservation

Yun Zhao, Qiong Jin, Zi-Jiao Wang, Xing-Yu Tao, Xiao-Dong Luo. Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine[J]. 应用天然产物, 2023, 13(4): 25-25.

Yun Zhao, Qiong Jin, Zi-Jiao Wang, Xing-Yu Tao, Xiao-Dong Luo. Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine[J]. Natural Products and Bioprospecting, 2023, 13(4): 25-25.

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Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine

Quality assurance of postharvest grapes against Botrytis cinerea by terbinafine

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