New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

doi:10.1007/s13659-015-0077-z

应用天然产物 ›› 2015, Vol. 5 ›› Issue (6): 277-285.DOI: 10.1007/s13659-015-0077-z

New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

Daniela Batista¹, Pedro L. Falé^1,2, Maria L. Serralheiro¹, Maria E. Araújo¹, Paulo J. A. Madeira¹, Carlos Borges¹, Isabel Torgal³, Margarida Goulart³, Jorge Justino³, Alice Martins¹, Amélia P. Rauter¹

1. Centro de Quĺmica e Bioquĺmica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal;
2. Institute of Pharmaceutical Science, King's College London, 150 Stamford Street, London SE19 NH, UK;
3. Escola Superior Agrária de Santarém, Quinta do Galinheiro, 2001-904 Santarém, Portugal

收稿日期:2015-09-01 修回日期:2015-10-14 出版日期:2015-12-24 发布日期:2018-02-11
通讯作者: Alice Martins,e-mail:aimartins@fc.ul.pt;aprauter@fc.ul.pt
基金资助:
The authors gratefully acknowledge Funda??o para a Ciência e a Tecnologia for financial support of the projects PTDC/QUI/67165/2006 and PEst-OE/QUI/UI0612/2013. The authors also thank the European Commission for approval of the INOVAFUNAGEING commitment and the support of the project "PER-sonalised ICT supported Service for Independent Living and Active Ageing", FP7-ICT-2013-10, Project Nr. 610359, 2013-2016. The collaboration of the staff from the Herbarium of Jardim Botanico da Madeira, for providing plant material, is also acknowledged.

New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

Daniela Batista¹, Pedro L.Falé^1,2, Maria L.Serralheiro¹, Maria E.Araújo¹, Paulo J.A.Madeira¹, Carlos Borges¹, Isabel Torgal³, Margarida Goulart³, Jorge Justino³, Alice Martins¹, Amélia P.Rauter¹

1. Centro de Quĺmica e Bioquĺmica, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisbon, Portugal;
2. Institute of Pharmaceutical Science, King's College London, 150 Stamford Street, London SE19 NH, UK;
3. Escola Superior Agrária de Santarém, Quinta do Galinheiro, 2001-904 Santarém, Portugal

Received:2015-09-01 Revised:2015-10-14 Online:2015-12-24 Published:2018-02-11
Supported by:
The authors gratefully acknowledge Funda??o para a Ciência e a Tecnologia for financial support of the projects PTDC/QUI/67165/2006 and PEst-OE/QUI/UI0612/2013. The authors also thank the European Commission for approval of the INOVAFUNAGEING commitment and the support of the project "PER-sonalised ICT supported Service for Independent Living and Active Ageing", FP7-ICT-2013-10, Project Nr. 610359, 2013-2016. The collaboration of the staff from the Herbarium of Jardim Botanico da Madeira, for providing plant material, is also acknowledged.

摘要/Abstract

摘要： The inhibition of α-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera(Fabaceae). This extract decreased a-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25%, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06%. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme(47.5% inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher(67.82%) than that promoted by ascorbic acid, the positive control(45.05%). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC-DAD-ESI-MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.

关键词: α-Glucosidase inhibition, In vitro digestion, HPLC-DAD-ESI-MS/MS, Antioxidant activity, Flavonoid glycosides

Abstract: The inhibition of α-glucosidase and glucose-6-phosphatase, two enzymes involved in the carbohydrate metabolism, is an important target to control glycaemia on individuals with type 2 diabetes. In this work we report for the first time the inhibition of both enzymes by the antihyperglycemic n-butanol extract from Genista tenera(Fabaceae). This extract decreased a-glucosidase and glucose-6-phosphatase activities to 0.97 and 80.25%, respectively, being more effective than acarbose, and phlorizin, the positive controls, which reduced enzymes activities only to 17.39 and 96.06%. Once inflammation and oxidative stress are related to diabetic impairments, the anti-inflammatory activity of the extract was also evaluated, through its inhibitory activity over COX-1 enzyme(47.5% inhibition). Moreover, after induction of oxidative stress by UV radiation, the viability of irradiated rat liver hepatoma cells exposed to the extract was significantly higher(67.82%) than that promoted by ascorbic acid, the positive control(45.05%). In addition, the stability of the extract under gastrointestinal conditions was evaluated by HPLC-DAD-ESI-MS/MS. Flavonoid diglycosides were identified as the main constituents of the extract, and no alterations in the chemical composition nor in the antioxidant activity were observed after in vitro digestion with artificial gastric and pancreatic juices.

Key words: α-Glucosidase inhibition, In vitro digestion, HPLC-DAD-ESI-MS/MS, Antioxidant activity, Flavonoid glycosides

Daniela Batista, Pedro L. Falé, Maria L. Serralheiro, Maria E. Araújo, Paulo J. A. Madeira, Carlos Borges, Isabel Torgal, Margarida Goulart, Jorge Justino, Alice Martins, Amélia P. Rauter. New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract[J]. 应用天然产物, 2015, 5(6): 277-285.

Daniela Batista,Pedro L.Falé,Maria L.Serralheiro,Maria E.Araújo,Paulo J.A.Madeira,Carlos Borges,Isabel Torgal,Margarida Goulart,Jorge Justino,Alice Martins,Amélia P.Rauter. New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract[J]. Natural Products and Bioprospecting, 2015, 5(6): 277-285.

参考文献

1. E. Nicolle, F. Souard, P. Faure, A. Boumendjel, Curr. Med. Chem. 18, 2661-2672 (2011)
2. A. Dey, J. Lakshmanan, Food Funct. 4, 1148-1184 (2013)
3. K. Kawaguchi, T. Matsumoto, Y. Kumazawa, Curr. Top. Med. Chem. 11, 1767-1779 (2011)
4. G.B. Bubols, D.D. Vianna, A. Medina-Remon, G. von Poser, R.M. Lamuela-Raventos, V.L. Eifler-Lima, S.C. Garcia, MiniRev. Med. Chem. 13, 318-334 (2013)
5. P.V.A. Babu, D.M. Liu, E.R. Gilbert, J. Nutr. Biochem. 24, 1777-1789 (2013)
6. R. Singh, N. Kaur, L. Kishore, G.K. Gupta, J. Ethnopharmacol. 150, 51-70 (2013)
7. G.S. Prasath, S.I. Pillai, S.P. Subramanian, Eur. J. Pharmacol. 740, 248-254 (2014)
8. W. Jiang, H. Kan, P. Li, S. Liu, Z. Liu, Anal. Methods 7, 123-128 (2015)
9. A.P. Rauter, A. Martins, R. Lopes, J. Ferreira, M.L.M. Serralheiro, M.E. Araújo, C. Borges, J. Justino, F.V. Silva, M. Goulart, J. Thomas-Oates, J.A. Rodrigues, E. Edwards, J.P. Noronha, R. Pinto, H. Mota-Filipe, J. Ethnopharmacol. 122, 384-393 (2009)
10. R. Tundis, M.R. Loizzo, F. Menichini, Mini-Rev. Med. Chem. 10, 1-14 (2010)
11. T.T. Mai, N.N. Thu, P.G. Tien, N.V. Chuyen, J. Nutr. Sci. Vitaminol. 53, 267-276 (2007)
12. P.S.M. Prince, N. Kamalakkannan, J. Biochem. Mol. Toxicol. 20, 96-102 (2006)
13. O. Estrada, M. Hasegawa, F. Gonzalez-Mujíca, N. Motta, E. Perdomo, A. Solorzano, J. Méndez, B. Méndez, G. Zea, Phytother. Res. 19, 859-863 (2005)
14. H. Horai, M. Arita, S. Kanaya, Y. Nihei, T. Ikeda, K. Suwa, Y. Ojima, K. Tanaka, S. Tanaka, K. Aoshima, Y. Oda, Y. Kakazu, M. Kusano, T. Tohge, F. Matsuda, Y. Sawada, M.Y. Hirai, H. Nakanishi, K. Ikeda, N. Akimoto, T. Maoka, H. Takahashi, T. Ara, N. Sakurai, H. Suzuki, D. Shibata, S. Neumann, T. Iida, K. Tanaka, K. Funatsu, F. Matsuura, T. Soga, R. Taguchi, K. Saito, T. Nishioka, MassBank:a public repository for sharing mass spectral data for life sciences. J. Mass Spectrom. 45, 703-714 (2010)
15. K. Ablajan, J. Mass Spectrom. 46, 77-84 (2011)
16. P. Waridel, J.L. Wolfender, K. Ndjoko, K.R. Hobby, H.J. Major, K. Hostettmann, J. Chromatogr. A 926, 29-41 (2001)
17. R. Simons, J.P. Vincken, M.C. Bohin, T.F.M. Kuijpers, M.A. Verbruggen, H. Gruppen, Rapid Commun. Mass Spectrom. 25, 55-65 (2011)
18. P.A. Madeira, M.H. Florêncio, C.M. Borges, Rapid Commun. Mass Spectrom. 24, 3432-3440 (2010)
19. P.L. Falé, C. Ferreira, A.M. Rodrigues, P. Cleto, P.J.A. Madeira, M.H. Florêncio, F.N. Frazao, M.L. Serralheiro, J. Med. Plants Res. 20, 1370-1378 (2013)
20. R. Kostiainen, T.J. Kauppila, J. Chromatogr. A 1216, 685-699 (2009)
21. J.P. Rauha, H. Vuorela, R. Kostiainen, J. Mass Spectrom. 36, 1269-1280 (2001)
22. M.J. Simirgiotis, G. Schmeda-Hirschmann, J. Bórquez, E. Kennelly, Molecules 18, 1672-1692 (2013)
23. J. Han, M. Ye, X. Qiao, M. Xu, B.R. Wang, D.A. Guo, J. Pharm. Biomed. Anal. 47, 516-525 (2008)
24. Y. Wang, L. Yang, Y.Q. He, C.H. Wang, E.W. Welbeck, S.W.A. Bligh, Z.T. Wang, Rapid Commun. Mass Spectrom. 22, 1767-1778 (2008)
25. J.B. Peng, H.M. Jia, Y.T. Liu, H.W. Zhang, S. Dong, Z.M. Zou, J. Pharm. Biomed. Anal. 55, 984-995 (2011)
26. O.L. Marcucci, F. Gonzalez-Mujica, E. Perez-Ayuso, Acta Cient. Venez. 34, 109-117 (1983)
27. N. Amessis-Ouchemoukh, K. Madani, P.L.V. Falé, M.L. Serralheiro, M.E.M. Araújo, Ind. Crop Prod. 53, 6-15 (2014)
28. European Centre for the Validation of Alternative Methods, ECVAM, Invit. Protoc. 17, 1-6 (1990)

[1]	Srijan Banerjee, Gustavo Cabrera-Barjas, Jaime Tapia, João Paulo Fabi, Cedric Delattre, Aparna Banerjee. Characterization of Chilean hot spring-origin Staphylococcus sp. BSP3 produced exopolysaccharide as biological additive[J]. 应用天然产物, 2024, 14(2): 3-3.
[2]	Teresa S. Catalá, Linn G. Speidel, Arlette Wenzel-Storjohann, Thorsten Dittmar, Deniz Tasdemir. Bioactivity profile of dissolved organic matter and its relation to molecular composition[J]. 应用天然产物, 2023, 13(5): 32-32.
[3]	Luan Wen, Zhou-Wei Wu, Li-Wu Lin, Abdulbaset Al-Romaima, Xing-Rong Peng, Ming-Hua Qiu. Structural characterizations and α-glucosidase inhibitory activities of four Lepidium meyenii polysaccharides with different molecular weights[J]. 应用天然产物, 2023, 13(3): 18-18.
[4]	Manar Adam, Gihan O. M. Elhassan, Sakina Yagi, Fatma Sezer Senol, Ilkay Erdogan Orhan, Abdel Azim Ahmed, Thomas Efferth. In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan[J]. 应用天然产物, 2018, 8(2): 97-105.
[5]	Shan Zhang, Lu Xu, Yang-Xi Liu, Hai-Yan Fu, Zuo-Bing Xiao, Yuan-Bin She. Characterization of Aroma-Active Components and Antioxidant Activity Analysis of E-jiao (Colla Corii Asini) from Different Geographical Origins[J]. 应用天然产物, 2018, 8(2): 71-82.

New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

New In Vitro Studies on the Bioprofile of Genista tenera Antihyperglycemic Extract

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 5

编辑推荐

Metrics

本文评价