Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

doi:10.1007/s13659-023-00408-w

应用天然产物 ›› 2023, Vol. 13 ›› Issue (6): 41-41.DOI: 10.1007/s13659-023-00408-w

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Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

Yue Song^1,4, Qi-Yang Li^1,3, Meng-Jing Cong^1,4, Xiao-Yan Pang¹, Bo Chen², Yong-Hong Liu^1,4,5, Li Liao^2,6, Jun-Feng Wang^1,4,5

1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine, Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;
2. Key Laboratory for Polar Science, MNR, Polar Research Institute of China, Shanghai, 200136, China;
3. Department of Pharmacology and Therapeutics, McGill University, Montreal, H3A 0G4, Canada;
4. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China;
5. Sanya Institute of Marine Ecology and Engineering, Sanya, 572000, China;
6. School of Oceanography, Shanghai Jiao Tong University, Shanghai, 200240, China

收稿日期:2023-07-30 出版日期:2023-12-24 发布日期:2023-12-26
通讯作者: Li Liao,E-mail:liaoli@pric.org.cn;Jun-Feng Wang,E-mail:wangjunfeng@scsio.ac.cn
基金资助:
This research was supported by the Guangdong Basic and Applied Basic Research Foundation (2021A1515011523, 2021B1515120046, 2022B1515120075), National Key Research and Development Program of China (2022YFC2807501), National Natural Science Foundation of China (grant no. 41976224), Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (2021JJLH0097, 2021CXLH0013), Hainan Provincial Natural Science Foundation of China (823CXTD393). We thank ZH Xiao, AJ Sun, XH Zheng, X Ma, and Y Zhang in the analytical facility at SCSIO for recording spectroscopic data.

Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

Yue Song^1,4, Qi-Yang Li^1,3, Meng-Jing Cong^1,4, Xiao-Yan Pang¹, Bo Chen², Yong-Hong Liu^1,4,5, Li Liao^2,6, Jun-Feng Wang^1,4,5

1. CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine, Materia Medica/Innovation Academy of South China Sea Ecology and Environmental Engineering, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;
2. Key Laboratory for Polar Science, MNR, Polar Research Institute of China, Shanghai, 200136, China;
3. Department of Pharmacology and Therapeutics, McGill University, Montreal, H3A 0G4, Canada;
4. University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China;
5. Sanya Institute of Marine Ecology and Engineering, Sanya, 572000, China;
6. School of Oceanography, Shanghai Jiao Tong University, Shanghai, 200240, China

Received:2023-07-30 Online:2023-12-24 Published:2023-12-26
Contact: Li Liao,E-mail:liaoli@pric.org.cn;Jun-Feng Wang,E-mail:wangjunfeng@scsio.ac.cn
Supported by:
This research was supported by the Guangdong Basic and Applied Basic Research Foundation (2021A1515011523, 2021B1515120046, 2022B1515120075), National Key Research and Development Program of China (2022YFC2807501), National Natural Science Foundation of China (grant no. 41976224), Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City (2021JJLH0097, 2021CXLH0013), Hainan Provincial Natural Science Foundation of China (823CXTD393). We thank ZH Xiao, AJ Sun, XH Zheng, X Ma, and Y Zhang in the analytical facility at SCSIO for recording spectroscopic data.

摘要/Abstract

摘要： Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC₅₀ values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6-8 exhibited inhibitory activities with IC₅₀ values of 10.88±3.05, 38.88±3.29, and 2.44±0.17 μg/mL, respectively (IC₅₀ 91.6 μM for the positive control loratadine).

关键词: Nocardiopsis dassonvillei, Arctic Ocean, Phenazine, Phenoxazine, Cytotoxic, Antiallergic

Abstract: Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC₅₀ values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6-8 exhibited inhibitory activities with IC₅₀ values of 10.88±3.05, 38.88±3.29, and 2.44±0.17 μg/mL, respectively (IC₅₀ 91.6 μM for the positive control loratadine).

Key words: Nocardiopsis dassonvillei, Arctic Ocean, Phenazine, Phenoxazine, Cytotoxic, Antiallergic

Yue Song, Qi-Yang Li, Meng-Jing Cong, Xiao-Yan Pang, Bo Chen, Yong-Hong Liu, Li Liao, Jun-Feng Wang. Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F[J]. 应用天然产物, 2023, 13(6): 41-41.

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Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

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