2018年, 第8卷, 第2期　
刊出日期：2018-04-24

  

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ORIGINAL ARTICLES
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  Characterization of Aroma-Active Components and Antioxidant Activity Analysis of E-jiao (Colla Corii Asini) from Different Geographical Origins

  Shan Zhang, Lu Xu, Yang-Xi Liu, Hai-Yan Fu, Zuo-Bing Xiao, Yuan-Bin She

  应用天然产物. 2018, 8(2): 71-82. https://doi.org/10.1007/s13659-017-0149-3

  摘要 ( 7690) PDF全文 ( 15402 )   可视化   收藏

  E-jiao (Colla Corii Asini, CCA) has been widely used as a healthy food and Chinese medicine. Although authentic CCA is characterized by its typical sweet and neutral fragrance, its aroma components have been rarely investigated. This work investigated the aroma-active components and antioxidant activity of 19 CCAs from different geographical origins. CCA extracts obtained by simultaneous distillation and extraction were analyzed by gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O) and sensory analysis. The antioxidant activity of CCAs was determined by ABTS and DPPH assays. A total of 65 volatile compounds were identified and quantified by GC-MS and 23 aroma-active compounds were identified by GC-O and aroma extract dilution analysis. The most powerful aroma-active compounds were identified based on the flavor dilution factor and their contents were compared among the 19 CCAs. Principal component analysis of the 23 aroma-active components showed 3 significant clusters. Canonical correlation analysis between antioxidant assays and the 23 aroma-active compounds indicates strong correlation (r=0.9776, p=0.0281). Analysis of aroma-active components shows potential for quality evaluation and discrimination of CCAs from different geographical origins.
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  Chemical Profile of the Sulphated Saponins from the Starfish Luidia senegalensis Collected as by-Catch Fauna in Brazilian Coast

  Marcelo Marucci Pereira Tangerina, Júlia Pizarro Cesário, Gerson Rodrigues Raggi Pereira, Tania Márcia Costa, Wagner Cotroni Valenti, Wagner Vilegas

  应用天然产物. 2018, 8(2): 83-89. https://doi.org/10.1007/s13659-018-0153-2

  摘要 ( 7425) PDF全文 ( 12062 )   可视化   收藏

  The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value. In order to try to add some value to these materials, we analyzed the chemical composition of the starfish Luidia senegalensis collected in the Brazilian coast as a consequence of the trawling fishery method. In order to access their chemical composition, we used a combination of solid phase extraction (SPE) followed by ultra-high performance liquid chromatography coupled to electrospray ionization ion trap tandem mass spectrometry (UPLC-ESI-IT-MS_n). Luidia senegalensis contains asterosaponins, which are sulphated glycosilated steroids, containing five and six sugar moieties, in addition to polyhydroxysteroids. This study helped us to support the presence of important and potentially bioactive compounds in invertebrates associated to the by-catch fauna of the shrimp fishery, using a fast and efficient method.
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  Xylanilyticolides A-C, Three New Compounds from Cultures of the Actinomycete Promicromonospora xylanilytica YIM 61515

  Zhen-Xiong Wang, Shen Qin, Li-Hua Xu, He-Ping Chen, Huan Sun, Rong Huang, Zheng-Hui Li, Tao Feng, Ji-Kai Liu

  应用天然产物. 2018, 8(2): 91-95. https://doi.org/10.1007/s13659-018-0154-1

  摘要 ( 8165) PDF全文 ( 12266 )   可视化   收藏

  Three new lactones, xylanilyticolides A-C (1-3), were isolated from cultures of the actinomycete Promicromonospora xylanilytica YIM 61515. Their structures were elucidated by 1D and 2D NMR spectroscopic data in conjunction with HRESIMS analysis. Compound 1 exhibited potent cytotoxicities against five human cancer cell lines HL-60, A-549, SMMC-7721, MCF-7 and SW480 with the IC₅₀ values of 3.9, 15.2, 11.2, 5.9, and 4.7 μM, respectively.
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  In Vitro Antioxidant and Cytotoxic Activities of 18 Plants from the Erkowit Region, Eastern Sudan

  Manar Adam, Gihan O. M. Elhassan, Sakina Yagi, Fatma Sezer Senol, Ilkay Erdogan Orhan, Abdel Azim Ahmed, Thomas Efferth

  应用天然产物. 2018, 8(2): 97-105. https://doi.org/10.1007/s13659-018-0155-0

  摘要 ( 7801) PDF全文 ( 11716 )   可视化   收藏

  We investigated the antioxidant potential and cytotoxicity towards human CCRF-CEM leukemia cells of 57 extracts obtained from 18 plants collected in the Erkowit region, eastern Sudan. The antioxidant activity was determined by measuring the radical scavenging effects against 2,2-diphenyl-1-picrylhydrazyl (DPPH) and N,N-dimethyl-p-phenylendiamine (DMPD), metal-chelation capacity, ferric-reducing (FRAP) and phosphomolibdenum-reducing antioxidant power (PRAP) methods using ELISA microtiter assays. Total phenol and flavonoid amounts of the extracts were determined spectrophotometrically. Cytotoxicity towards CCRF-CEM cells was evaluated by the resazurin reduction assay. Geranium favosum followed by Kalanchoe glaucescens, Malva parviflora, Aizoon canariense, and Coleus barbatus, respectively, possessed the highest antioxidant activity among the studied plants. Chrozophora oblongifolia and K. glaucescens exerted considerable cytotoxicity against CCRF-CEM leukemia cells. These plants may serve as source for the further development of natural antioxidant and antitumor agents.
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  Two New Indolyl Diketopiperazines, Trypostatins C and D from Aspergillus penicilliodes Speg.

  Han Zhang, Hong-Tao Zhu, Dong Wang, Chong-Ren Yang, Ying-Jun Zhang

  应用天然产物. 2018, 8(2): 107-111. https://doi.org/10.1007/s13659-018-0156-z

  摘要 ( 7184) PDF全文 ( 11688 )   可视化   收藏

  Twelve indolyl diketopiperazines 1-12 were isolated from the mycelia culture of Aspergillus penicilliodes Speg., a dominant microorganism from the post fermentation process of ripe Pu-er tea. Their structures were elucidated by extensive spectroscopic methods. Among them, trypostatins C (1) and D (2) featuring with a rare methyl vinyl ketone side chain at C-2 are new compounds, while 3 and 4 were obtained for the first time from nature source. The isolates 3-12 did not show obvious cytotoxicities against five human cancer cell lines at a concentration of 40 μM.
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  Seco-tremulane Sesquiterpenoids from the Cultures of the Medicinal Fungus Irpex lacteus HFG1102

  He-Ping Chen, Zhen-Zhu Zhao, Zheng-Hui Li, Tao Feng, Ji-Kai Liu

  应用天然产物. 2018, 8(2): 113-119. https://doi.org/10.1007/s13659-018-0157-y

  摘要 ( 7414) PDF全文 ( 11202 )   可视化   收藏

  Six previously undescribed 5,6-seco-tremulane analogues, together with two known ones, were isolated from the culture broth of the medicinal fungus Irpex lacteus HFG1102. The structures of the new compounds were elucidated via extensive spectroscopic methods, including NMR and HRMS spectroscopic analyses.
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  Secondary Metabolites from the Endophytic Fungus Xylaria sp. Hg1009

  Rong Chen, Jian-Wei Tang, Xing-Ren Li, Miao Liu, Wen-Ping Ding, Yuan-Fei Zhou, Wei-Guang Wang, Xue Du, Han-Dong Sun, Pema-Tenzin Puno

  应用天然产物. 2018, 8(2): 121-129. https://doi.org/10.1007/s13659-018-0158-x

  摘要 ( 7229) PDF全文 ( 9710 )   可视化   收藏

  A detailed chemical investigation of the secondary metabolites produced by the endophytic fungus Xylaria sp. isolated from the stems of Isodon sculponeatus afforded six new compounds, xylariahgins A-F (1-6), two new natural products (7 and 8), along with two known compounds (9 and 10) (Fig. 1). The structures of all compounds were unambiguously established by analyzing their spectroscopic data or referring to pertinent literature. Compounds 1-8 were tested for their cytotoxic activity against five human tumor cell lines.
SHORT COMMUNICATION
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  Abietane Diterpernoids from the Roots of Euphorbia ebracteolata

  Yuan-Liang Ma, Xiao-Han Tang, Wen-Juan Yuan, Xiao Ding, Ying-Tong Di, Xiao-Jiang Hao

  应用天然产物. 2018, 8(2): 131-135. https://doi.org/10.1007/s13659-018-0159-9

  摘要 ( 5999) PDF全文 ( 7856 )   可视化   收藏

  A new ent-abietane diterpernoid, named ebracteolata D (1), along with 11 known analogues, was isolated from the roots of Euphorbia ebracteolata Hayata. The structure of 1 was elucidated on the basis of spectroscopic analysis and molecular modeling. Cytotoxicity of compounds 1-12 was evaluated as well as the effect on the NF-κB pathway. Among them, compound 12, jolkinolide B, displayed broad inhibitory effects against proliferation of tumor cell lines. Mechanistic studies indicated that the compound 12 can inhibit TNF-α induced NF-κB activation, thereby inducing tumor cell apoptosis.