Physiological plasticity in eucalyptus clones in the vegetative stage contributes to drought tolerance

doi:10.1007/s11676-022-01571-5

Abstract

Abstract:

With the expansion of eucalyptus crops to areas with severe water limitations, physiological studies involving eucalyptus clones to identify those that are tolerant to water stress become important. The objective of this study was to assess morphological and physiological responses by eucalyptus clones subjected to drought stress and rehydration. The experiment consisted of three eucalyptus clones: VC865, I224 and I144 and two water regimes: control and water stress followed by rehydration, with six replicates. Leaf water potential, gas exchange, maximum quantum efficiency of photosystem II and plant height and stem diameter were evaluated under drought stress and rehydration. After 6 d of rehydration, the number of leaves, leaf area and dry mass of root, leaf, stem and their total were evaluated. All clones showed intense reduction of gas exchange during the drought stress period, and only VC865 and I144 showed rapid recovery with 3 d of rehydration. Clone I224 showed greater reduction in height, stem diameter, number of leaves, water potential at midday (Ψ _{w Midday}), and maximum quantum efficiency of photosystem II (F _v/F _m). Clones VC865 and I144 showed lower reductions in Ψ _{w Midday} and F _v/F _m under stress. VC865 had lower reductions in leaf number, leaf area and higher leaf dry mass, while clone I144 had higher height and lower reduction in root dry mass under. Both these clones showed higher water use efficiency with 3 d of rehydration. These different phenotypic plasticities gave the clones VC865 and I144 efficient mechanisms of acclimatization to stress and more drought tolerance, enhancing their greater capacity for recovery after stress, which allowed lower dry mass reduction. Clone I224, however, was more susceptible to drought stress, undergoing greater physiological damage with only partial recovery during rehydration.

Key words: Drought, Eucalyptus species, Gas exchanges, Post-stress, Root dry mass

Vilma Marques Ferreira, Jailma Ribeiro de Andrade, Claudiana Moura dos Santos, Sebastião de Oliveira Maia Júnior, João Correia de Araújo Neto, Andrea Francisca da Silva Santos, Vicente Mota da Silva, Lucas Teles Bezerra, Laurício Endres. Physiological plasticity in eucalyptus clones in the vegetative stage contributes to drought tolerance[J]. JOURNAL OF FORESTRY RESEARCH, 2023, 34(5): 1549-1561.

Vilma Marques Ferreira, Jailma Ribeiro de Andrade, Claudiana Moura dos Santos, Sebastião de Oliveira Maia Júnior, João Correia de Araújo Neto, Andrea Francisca da Silva Santos, Vicente Mota da Silva, Lucas Teles Bezerra, Laurício Endres. [J]. 林业研究(英文版), 2023, 34(5): 1549-1561.

References 36

1	Amrutha S, Muneera PAB, Muthupandi M, Sivakumar V, Nautiyal R, Dasgupta MG. Variation in morpho-physiological, biochemical and molecular responses of two Eucalyptus species under short-term water stress. Acta Bot Croat, 2019, 78(2): 125-134, DOI
2	Chen X, Zhao P, Ouyang L, Zhu L, Ni G, Schäfer KV. Whole-plant water hydraulic integrity to predict drought-induced Eucalyptus urophylla mortality under drought stress. For Ecol Manag, 2020, 468, DOI
3	R CORE TEAM (2015) A language and environment for statistical computing. R. Foundation for Statistical Computing, Vienna, Austria. Available from: http://www.R-project.org/. Accessed 25 June 2021
4	Correia B, Pintó-Marijuan M, Neves L, Brossa R, Dias MC, Costa A, Araújo C, Santos C, Chaves MM, Pinto G. Water stress and recovery in the performance of two Eucalyptus globulus clones: physiological and biochemical profiles. Physiol Plant, 2014, 150(4): 580-592, DOI
5	Correia B, Hancock RD, Amaral J, Cadenas AG, Valledor L, Pinto G. Combined drought and heat activates protective responses in Eucalyptus globulus that are not activated when subjected to drought or heat stress alone. Front Plant Sci, 2018, 9: 819, DOI
6	Cruz CD, Regazzi AJ. Modelos biométricos aplicados no melhoramento genético, 1994 Viçosa ImprensaUniversitária UFV 390p
7	Endres L, Santos CM, Souza GV, Menossi M, Santos JCM (2018) Morphological changes recorded in different phenophases of sugarcane plants subjected to water stress in tropical field conditions. Aust J Crop Sci 12(7): 1041−1050. https://doi.org/10.3316/informit.881611792651534
8	Favreau B, Denis M, Ployet R, Mounet F, Peireira SH, Franceschini L, Laclau J-P, Labate C, Carrer H. Distinct leaf transcriptomic response of water deficient Eucalyptus grandis submitted to potassium and sodium fertilization. PLoS ONE, 2019, 14: 1-30, DOI
9	Fernandes ET, Cairo PAR, Morais JLC. Crescimento de clones de eucalipto sob deficiência hídrica. Enciclopédiabiosfera, 2013, 9(17): 870
10	Ferraz AC, Mola-Yudego B, Gonzalez-Olabarria JR, Scolforo JRS. Thinning regimes and initial spacing for Eucalyptus plantations in Brazil. An Acad Bras Cienc, 2018, 90: 255-265, DOI
11	Ferreira DF. Sisvar: a computer statistical analysis system. Ciênc Agrotec, 2011, 35: 1039-1042, DOI
12	Frosi G, Harand W, Oliveira MTD, Pereira S, Cabral SP, Montenegro AADA, Santos MG. Different physiological responses under drought stress result in different recovery abilities of two tropical woody evergreen species. Acta Bot Bras, 2017, 31: 153-160, DOI
13	Hamed SB, Lefi E, Chaieb M. Growth phenology of pistachio seedlings under water stress and rehydration conditions. J Plant Biol Crop Res, 2022, 5(1): 1060
14	Hayat U, Jilani MI, Rehman R, Nadeem F. A review on Eucalyptus globulus: a new perspective in the rapeutics. Int J ChemBiochemSci, 2015, 8: 85-91
15	IBA 2020 IBA Relatório (2020) Indústria Brasileira de Árvores, Pöyry Consultoria em Gestão e Negócios Ltda., Brazil, pp. 66. Available from: https://iba.org/datafiles/publicacoes/relatorios/relatorio-iba-2020.pdf. Accessed 28 July 2001.
16	Kapoor D, Bhardwaj S, Landi M, Sharma A, Ramakrishnan M, Sharma A. The impact of drought in plant metabolism: how to exploit tolerance mechanisms to increase crop production. Appl Sci, 2020, 10(16): 5692, DOI
17	Kijowska-Oberc J, Staszak AM, Kamiński J, Ratajczak E. Adaptation of forest trees to rapidly changing climate. Forests, 2020, 11(2): 123, DOI
18	Larcher, W (2006) Ecofisiologia vegetal. Traducao: PRADO, C.H.B.A. Sao Carlos: Rima. 531p.
19	Marchiori PE, Machado EC, Sales CR, Espinoza-Núñe E, Magalhães Filho JR, Souza GM, Pires RCM, Ribeiro RV. Physiological plasticity is important for maintaining sugarcane growth under water deficit. Front Plant Sci, 2017, 8: 2148, DOI
20	Maxwell MO, Johnson GN. Chlorophyll fluorescence: a practical guide. J Exp Bot, 2000, 51(345): 659-668, DOI
21	Müller C, Hodecker BER, de Barros NF, Merchant A. A physiological approach for pre-selection of Eucalyptus clones resistant to drought. iForest, 2020, 13(1): 16-23, DOI
22	Murchie EH, Niyogi KK. Manipulation of photoprotection to improve plant photosynthesis. Plant Physiol, 2011, 155(1): 86-92, DOI
23	Ocheltree TW, Nippert JB, Prasad PVV. Stomatal responses to changes in vapor pressure déficit reflect tissue-specific differences in hydraulic conductance. Plant Cell Environ, 2014, 37(1): 132-139, DOI
24	Pereira MRR, Klar AE, Filho ALM, Rodrigues ACR, Silva MR. Influência de diferentes condições de solo no desenvolvimento de plantas de Eucalyptus urograndis submetidas a déficit hídrico. Irriga, 2018, 12(4): 519-530, DOI
25	Pita-Barbosa A, NairamBerh Barros F. Boron as mitigator of drought damage in Eucalyptus: a genotype-dependent mechanism?. Sci For, 2016, 44(112): 851-861, DOI
26	Rehschuh R, Cecilia A, Zuber M, Faragó T, Baumbach T, Hartmann H . Drought-induced xylem embolism limits the recovery of leaf gas exchange in scots pine. Plant Physiol, 2020, 184(2): 852-864, DOI
27	Romeet S, Rai KC, Anugrah T, Haripriya K, Manoj R, Priya K, Kavidayal H, Raturi M, Kainthola C, Barthwal S. Differential responses in gas exchange variability, water use efficiency and chlorophyll content in Eucalyptus clones under drought stress. Indian for, 2019, 145(5): 459-466
28	Santos ICD, Almeida AAFD, Anhert D, Conceição ASD, Pirovani CP, Pires JL, Valle RR, Baligar VC. Molecular, physiological and biochemical responses of Theobroma cacao L. genotypes to soil water deficit. PLoS One, 2014, 9(12): e115746, DOI
29	Saravanan S. Gas exchange characteristics and water use efficiency in Eucalyptus clones. J Stress Physiol Biochem, 2018, 14: 49-58
30	Silva PH, Campoe OC, Paula RC, Lee DJ. Seedling growth and physiological responses of sixteen eucalypt taxa under controlled water regime. Forests, 2016, 7(6): 110, DOI
31	Souden S, Ennajeh M, Ouledali S, Massoudi N, Cochard H, Khemira H. Water relations, photosynthesis, xylem embolism and accumulation of carbohydrates and cyclitols in two Eucalyptus species (E. camaldulensis and E. torquata) subjected to dehydration-rehydration cycle. Trees, 2020, 34(6): 1439-1452, DOI
32	Sytsma KJ, Renner SS (2019) “Myrtales” Encyclopedia Britannica. Available from: https://www.britannica.com/plant/Myrtales. Accessed 8 July 2021
33	Valadares J, Paula NF, Paula RC. Physiological changes in eucalyptus hybrids under different irrigation regimes. Rev CiêncAgron, 2014, 45: 805-814
34	Valladares F, Chico JM, Aranda I, Balaguer L, Dizengremel P, Manrique E, Dreyer E. The greater seedling high-light tolerance of Quercus robur over Fagus sylvatica is linked to a greater physiological plasticity. Trees, 2002, 16(6): 395-403, DOI
35	Velikova V, Arena C, Izzo LG, Tsonev T, Koleva D, Tattini M, Roeva O, Maio A, Loreto F. Functional and structural leaf plasticity determine photosynthetic performances during drought stress and recovery in two Platanus orientalis populations from contrasting habitats. Int J Mol Sci, 2020, 21(11): 3912, DOI
36	Xiong S, Wang Y, Chen Y, Gao M, Zhao Y, Wu L. Effects of drought stress and rehydration on physiological and biochemical properties of four oak species in China. Plants, 2022, 11: 679, DOI

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