Abstract: Both genders of the dioecious gymnosperm Ginkgo biloba have distinct practical production and application uses, so quick, accurate identification of males and females is important for early seedling breeding. To develop a fast method to identify the sexes, we used the Easy DNA extraction (EZ-D) method to extract DNA from leaves within 1 min for use with the recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) system and identify the sex. A portable nucleic acid detection card kit (PNADCK) was used for on-site analysis. This method facilitates rapid extraction of nucleic acids from a single and can accurately detect 100 pg/µL of G. biloba female genomic DNA within 20 min at 39 °C. The EZ-DRPA-LFD-PNADCK system enables precise on-site determination of G. biloba leaf sex and is rapid, efficient, sensitive, and convenient, greatly enhancing productivity for G. biloba because seedlings with specific sex characteristics can be selected at an earlier stage, planting strategies can be optimized, and production efficiency improved.

Key words: Ginkgo biloba, RPA-LFD, Biosensor platform, Rapid diagnosis