Integrative Biology Journals

JOURNAL OF FORESTRY RESEARCH ›› 2025, Vol. 36 ›› Issue (1): 1-.DOI: 10.1007/s11676-025-01914-y

• Review Article •    

From lab to forest: overcoming barriers to in vitro propagation of forest trees

Tuija Aronen1, Valbona Sota2, Branislav Cvjetković3, Andrea Rupps5, Lucie Fischerova6, Bruce Christie4, Dheeraj Singh Rathore7, Stefaan P. O. Werbrouck8   

  1. 1Natural Resources Institute Finland, Vipusenkuja 5, 57200 Savonlinna, Finland 

    2Department of Biotechnology, Faculty of Natural Sciences, University of Tirana, Bul.”ZOG I”, No.2, 1001 Tirana, Albania

    3Faculty of Forestry, University of Banja Luka, Bulevar Vojvode Petra Bojovića 1A, 78000 Banja Luka, Bosnia and Herzegovina 

    4The Green Plant Co Ltd, 10 Newcastle St, Palmerston North 4410, New Zealand

    5Humboldt-Universität zu Berlin, Institute for Biology, AG Plant Evolution and Biodiversity, Späth-Arboretum, Späthstr. 80/81, 12437 Berlin, Germany 

    6Laboratory of Biologically Active Compounds, Institute of Experimental Botany of the Czech Academy of Sciences, Rozvojová 263, 165 00 Prague 6, Czech Republic 

    7Teagasc, Forestry Development Department, Oak Park, Carlow R93 XE12, Ireland 

    8Laboratory for Applied In Vitro Plant Biotechnology, Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, B-9000 Ghent, Belgium

  • Received:2025-05-01 Accepted:2025-06-08 Online:2025-09-05 Published:2025-01-01

Abstract: Reforestation initiatives are often limited by insufficient seeds, a problem exacerbated by natural variability in tree flowering and seed production and climate change and other environmental challenges. Innovative and adaptive solutions such as in vitro propagation are thus needed. Tissue culture can provide high-quality propagation material for tree conservation and mass propagation, but faces technical, economic, regulatory, and social barriers. Obstacles related to the academia–industry interface and to stakeholder concerns are discussed and actions suggested to overcome these barriers to realize the full potential of tree micropropagation. These include refining techniques to improve efficiency and reduce costs; establishing collaborations among researchers, industry, and foresters; and reducing points of contention and misinformation regarding genetic diversity and public perception. International collaborative initiatives, exemplified by the EU COST Action CA21157 COPYTREE, are elementary for achieving these goals.

Key words: Commercialization, Forest reproductive material, Technology transfer, Tissue culture