Abstract: In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata, the physicochemical properties, domains, conserved motifs, cis-acting elements, and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques, based on the full-length transcriptome data of the H. serrata. The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H. serrata, including 9 acidic proteins, 11 stabilizing proteins, 5 hydrophobic proteins, 7 proteins with transmembrane structures, and 3 proteins with signal peptides.(2) Subcellular localization was found in the cell wall, chloroplast, nucleus, and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs, and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA2 had the highest expressions in leaves, spores, stems and gemma. The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.

Key words: Huperzia serrata, COBRA gene family, bioinformatics, full-length transcriptome, expression analysis

摘要： 为探明蛇足石杉COBRA基因家族成员分子生物信息学特征及组织表达规律,该文基于蛇足石杉的全长转录组数据,通过生物信息学技术对该家族成员(HsCOBRAs)的理化性质、结构域、保守基序、顺式作用元件、基因表达量等进行分析。结果表明:(1)在蛇足石杉全长转录组中共筛选出24个HsCOBRAs家族成员,其中酸性蛋白9个,稳定蛋白11个,疏水性蛋白5个,具有跨膜结构的蛋白7个,具有信号肽的蛋白3个。(2)亚细胞定位在细胞壁、叶绿体、细胞核、细胞膜上。(3)结构分析发现HsCOBRAs有7种结构域和6种保守基序,部分成员具有高度保守的CCVS结构。(4)HsCOBRAs具有CAAT-box、TATA-box等45种顺式作用元件。(5)HsCOBRA2在叶、孢子、茎、芽胞中的表达量均最高。该研究结果可为HsCOBRAs的进一步研究及生物学功能验证等提供理论依据。

关键词: 蛇足石杉, COBRA基因家族, 生物信息学, 全长转录组, 表达分析