Abstract: The purification of protoplasts from cellular and subcellular debrids by floatable washing method with a modified centrifuge tube is described. In this method a modified centrifuge tube, which possesses opened narrow and short tube at the bottom, is used. The yield of protoplasts is relatively high by means of this purification method. The average numder of protoplasts obtained from the stems of corn (Zea mays L.) was 6.2 x 10^5 gm^-1 fresh weight. The average number of protoplasts obtained from the leaves of Petunia hybrida Vilm. was 2.7x10^6 gm^-1 fresh weight. After purification the viability of plant protoplasts is high. During progress the protoplasts were cultured cell division of corn stem protoplats was observed. Cultured protoplasts of Petunia leaves underwent cell division and developed into calli. After the calli were transferred onto differential medium the roots grew.

Key words: purification of plant, protoplast

摘要： 本文叙述了用改良离心管漂洗纯化原生质体中的细胞和亚细胞碎片的方法．在这方法中使用了一个通底的带有小细管的改良离心管．经过这种纯化，原生质体的产量是较高的．玉米（Zea mays L.）茎每克鲜重平均收获6.2x10^5个原生质体，矮牵牛(Petunia hybrida Vilm.）叶每克鲜重平均收获2.7X10 ^6个原生质体．纯化后植物原生质体的活力是较高的，当培养时，观察到玉米茎原生质体的细胞分裂；矮牵牛叶片原生质体进行了细胞分裂，并发育成愈伤组织，愈伤组织转移到分化培养基上长出了根．

关键词: 原生质体, 纯化