Abstract: The pollen tubes grown in vitro were rich in soluble polysaccharide, particularly in the top growth region at the initial stage of pollen tube develo -pment. The callos pluge gave a positive reaction to PAS. After entering the pollen tube, the generative cell approached the vegetative nucleus before long and sticked each other. Then, two sperms follmed via mitosis division. The intensity of colour in generative nucleus by using Feulgen reaction was deeper than vege- tative nucleus and two sperins forming later. The generative cell and vegetative nucleus gave a positive reaction to PAS stain. As ammnoniacal silver nitrite was used, the generative nucleus appeared yellow-black colour while vegetative nucleus gave a black reaction product. It suggested the existence of arginine-rich hi tone. After the formation of two sperms, the chromation of vegatative nucleus turned to diffusible and the vegtative nucleus made a very feeble response to the Fculgen reaction and A-S staining. The vegetdtive nuclcus was of tell in close proxity tothe sperms.

Key words: cxtochemical, localization, polysaccharide, reticulata

摘要： 人工培养的云南山茶(Camellia reticulata Lind1.)花粉管发育的早期含有较丰富的可溶性多糖，特别是顶端生长区。胼胝质拴塞显示PAS阳性反应。当生殖细胞进人花粉管后，在较短的时间内接近营养核，并与其相贴合。然后才开始有丝分裂形成两个精细胞。生殖细胞核孚尔根染色反应要比营养核以及后来形成的两个精核的染色深，生殖细胞与营养核PAS反应均为阳性。用氨一银染色法测定组蛋白的细胞化学所见，生殖核显示出黄黑色的反应产物，而营养核则显示有很丰富的精氨酸组蛋白黑色反应产物。在精子形成之后，营养核染色质扩散，孚尔根染色及组蛋白染色均很弱，并经常位于两精子的附近。

关键词: 山茶雄配子体, 发育过程, 多糖、DNA, 组蛋白, 化学定位