Abstract: Calli were induced from young stem and needle of Taxus chinensis (Pilg.) Rehd. Some basic respects of calli culture and cell suspension culture were investigated. Their capability of syn-thesis taxol were analyzed by HPLC method. Some factors of increasing the growth rate and taxol yield of cultured cells were tested. The growth rate of calli and suspension culture cells from Tax us chinensis were 0.25 g/L.d and 0.28 g / L.d (based on the cell dry weight) respectively, and their taxol content were 0.0026% and 0.012% respectively.

Key words: Taxus chinensis

摘要： 从红豆杉（Taxus chinensis（Pilg.）Rehd.）的嫩茎及针叶诱导出愈伤组织，对愈伤组织培养及细胞悬浮培养进行了研究。利用HPLC方法测定了它们合成紫杉醇的能力。发现了能够提高培养细胞生长速率及紫杉醇含量的一些因子。红豆杉愈伤组织及悬浮培养细胞的生长速率已分别达到0.25g/L.d和0.28g／L.d。而他们的紫杉醇含量分别是0.0026％和0.012％。

关键词: 红豆杉, 紫杉醇, 愈伤组织培养, 细胞悬浮培养