Abstract:

The composition, content, and structure of anthocyanins and flavonoids in purple-red, pink, and white petals of Bauhinia variegata L. were studied to lay a foundation for further investigations of the floral pigment mechanisms and molecular breeding of this species. The anthocyanins and flavonoids were analyzed using liquid chromatography-mass spectrometry (LC-MS/MS). In total, 53 anthocyanins and 340 flavonoids were detected. However, the anthocyanins were markedly different among the different colored petals. Malvidin-3-O-galactoside, malvidin-3-O-glucoside, delphinidin-3-O-galactoside were higher in the purple-red petals than in the white and pink petals. Delphinidin-3-O-(coumaryl)-glucoside, malvidin-3-O-sophoroside, petunidin-3-O-(coumaryl)-glucoside were higher in the pink petals than in the purple-red and white petals. Pelargonidin-3-O-(coumaryl)-glucoside was higher in the white petals than in the purple-red and pink petals. The flavonoids also differed significantly among the different petals. Pelargonidin-3-O-sambubioside-5-O-glucoside, pelargonidin-3-O-sophoroside, and delphinidin-3-O-sambubioside were almost not detected in the white petals and delphinidin-3-O-sophoroside was almost not detected in the purple-red petals, while peonidin-3-O-glucoside, peonidin-3,5-O-diglucoside, chrysoeriol-7-O-rutinoside-5-O-glucoside, and isovitexin-7-O-(6''-sinapoyl) glucoside were higher in the pink than in the white and purple-red petals.

摘要：

本研究选择 3 种不同花色的洋紫荆（Bauhinia variegata L.）为实验材料，应用LC-MS/MS技术分析花青素和类黄酮物质，比较不同花色洋紫荆之间的差异。结果显示，洋紫荆有53 种花青素和340 种类黄酮物质。不同花色洋紫荆花瓣中的花青素差异较大，紫红色花中的锦葵色素-3-O-半乳糖苷、锦葵色素-3-O-葡萄糖苷、飞燕草素-3-O-半乳糖苷等含量较高，粉红色花中的飞燕草素-3-O-对香豆酰葡萄糖苷、锦葵色素-3-O-槐糖苷、矮牵牛素-3-O-对香豆酰葡萄糖苷、锦葵色素-3-O-桑布双糖苷-5-葡萄糖苷等含量较高，白色花中的天竺葵素-3-O-对香豆酰葡萄糖苷等含量较高。白色花中几乎不含天竺葵素-3-O-桑布双糖苷、天竺葵素-3-O-槐糖苷、飞燕草素-3-O-桑布双糖苷等。紫红色花中几乎不含飞燕草素-3-O-槐糖苷。不同花色洋紫荆花瓣中的类黄酮差异较大，粉红色花中的芍药花素-3-O-葡萄糖苷、芍药花素-3,5-O-二葡萄糖苷、金圣草黄素-7-O-芸香糖苷-5-O-葡萄糖苷、异牡荆素-7-O-（6''-芥子酰）葡萄糖苷等含量较白色、紫红色花高。