Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut

doi:10.1016/j.pld.2024.03.010

Plant Diversity ›› 2024, Vol. 46 ›› Issue (03): 309-320.DOI: 10.1016/j.pld.2024.03.010

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Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut

Zi-Yan Zhang^a,b, He-Xiao Xia^c, Meng-Jie Yuan^a,b, Feng Gao^a,b, Wen-Hua Bao^a,b, Lan Jin^a,b, Min Li^a,b, Yong Li^a,b,d

a. College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010020, China;
b. Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China;
c. College of Landscape and Art, Henan Agricultural University, Zhengzhou 450002, China;
d. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China

Received:2024-01-05 Revised:2024-03-22 Online:2024-05-20 Published:2024-05-25
Contact: Yong Li,E-mail:20220053@imnu.edu.cn
Supported by:
We are very grateful to Professor Jin-Ling Huang of East Carolina University for his advice on our manuscript. This work was supported by National Natural Science Foundation of China (32360307), the Natural Science Foundation of Inner Mongolia (2023MS03031), Inner Mongolia Grassland Talents Project (3211002406), and the Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry) (Grant No. TGB2021004).

Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut

Zi-Yan Zhang^a,b, He-Xiao Xia^c, Meng-Jie Yuan^a,b, Feng Gao^a,b, Wen-Hua Bao^a,b, Lan Jin^a,b, Min Li^a,b, Yong Li^a,b,d

a. College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010020, China;
b. Key Laboratory of Biodiversity Conservation and Sustainable Utilization in Mongolian Plateau for College and University of Inner Mongolia Autonomous Region, Hohhot 010022, China;
c. College of Landscape and Art, Henan Agricultural University, Zhengzhou 450002, China;
d. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing 100091, China

通讯作者: Yong Li,E-mail:20220053@imnu.edu.cn
基金资助:
We are very grateful to Professor Jin-Ling Huang of East Carolina University for his advice on our manuscript. This work was supported by National Natural Science Foundation of China (32360307), the Natural Science Foundation of Inner Mongolia (2023MS03031), Inner Mongolia Grassland Talents Project (3211002406), and the Open Fund of State Key Laboratory of Tree Genetics and Breeding (Chinese Academy of Forestry) (Grant No. TGB2021004).

Abstract

Abstract: Chinese wingnut (Pterocarya stenoptera) is a medicinally and economically important tree species within the family Juglandaceae. However, the lack of high-quality reference genome has hindered its in-depth research. In this study, we successfully assembled its chromosome-level genome and performed multi-omics analyses to address its evolutionary history and synthesis of medicinal components. A thorough examination of genomes has uncovered a significant expansion in the Lateral Organ Boundaries Domain gene family among the winged group in Juglandaceae. This notable increase may be attributed to their frequent exposure to flood-prone environments. After further differentiation between Chinese wingnut and Cyclocarya paliurus, significant positive selection occurred on the genes of NADH dehydrogenase related to mitochondrial aerobic respiration in Chinese wingnut, enhancing its ability to cope with waterlogging stress. Comparative genomic analysis revealed Chinese wingnut evolved more unique genes related to arginine synthesis, potentially endowing it with a higher capacity to purify nutrient-rich water bodies. Expansion of terpene synthase families enables the production of increased quantities of terpenoid volatiles, potentially serving as an evolved defense mechanism against herbivorous insects. Through combined transcriptomic and metabolomic analysis, we identified the candidate genes involved in the synthesis of terpenoid volatiles. Our study offers essential genetic resources for Chinese wingnut, unveiling its evolutionary history and identifying key genes linked to the production of terpenoid volatiles.

Key words: Genome, Medicinal components, Metabolome, Pterocarya stenoptera, Transcriptome

摘要： Chinese wingnut (Pterocarya stenoptera) is a medicinally and economically important tree species within the family Juglandaceae. However, the lack of high-quality reference genome has hindered its in-depth research. In this study, we successfully assembled its chromosome-level genome and performed multi-omics analyses to address its evolutionary history and synthesis of medicinal components. A thorough examination of genomes has uncovered a significant expansion in the Lateral Organ Boundaries Domain gene family among the winged group in Juglandaceae. This notable increase may be attributed to their frequent exposure to flood-prone environments. After further differentiation between Chinese wingnut and Cyclocarya paliurus, significant positive selection occurred on the genes of NADH dehydrogenase related to mitochondrial aerobic respiration in Chinese wingnut, enhancing its ability to cope with waterlogging stress. Comparative genomic analysis revealed Chinese wingnut evolved more unique genes related to arginine synthesis, potentially endowing it with a higher capacity to purify nutrient-rich water bodies. Expansion of terpene synthase families enables the production of increased quantities of terpenoid volatiles, potentially serving as an evolved defense mechanism against herbivorous insects. Through combined transcriptomic and metabolomic analysis, we identified the candidate genes involved in the synthesis of terpenoid volatiles. Our study offers essential genetic resources for Chinese wingnut, unveiling its evolutionary history and identifying key genes linked to the production of terpenoid volatiles.

关键词: Genome, Medicinal components, Metabolome, Pterocarya stenoptera, Transcriptome

Zi-Yan Zhang, He-Xiao Xia, Meng-Jie Yuan, Feng Gao, Wen-Hua Bao, Lan Jin, Min Li, Yong Li. Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut[J]. Plant Diversity, 2024, 46(03): 309-320.

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Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut

Multi-omics analyses provide insights into the evolutionary history and the synthesis of medicinal components of the Chinese wingnut

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