An Ultrastructural Study of the Spermatogenesis of the Fern Pteris fauriei (Pteridaceae)

doi:10.3724/SP.J.1143.2010.10035

Abstract

Abstract:

The ultrastructural research reveals that the spermatogenesis of the fern Pteris fauriei undergoes the de novo occurrence of the motile organelles, including the blepharoplast, multilayered structure and flagella, the formation of the coiled mitochondrion and nuclear shaping, and finally forms a spiral spermatozoid, which resemble other homosporous ferns described. Some new phenomenon or details during spermatid differentiation in present study including: Spermatids show polar differentiation in the early stage, the nucleus lies in the proximal end (the end of cell plate formation in mitosis), and the blepharoplast, mitochondria and plastids mainly located in the distal end. Abundant microtubules extend from the blepharoplast and plenty of radial mitochondria surround the blepharoplast. The differentiation of the basal bodies undergoes 3 stages, ie. procentriole, centriole and basal body. Their internal structures show different patterns, which are the tubular pattern, cartwheel pattern, and stellate pattern respectively. The amorphous material in the blepharoplast is considered to be the microtubule organizer, from which the multilayered structure, the accessory microtubular ribbon and flagella are differentiated. Abundant membranous structures are produced in the course of spermatid differentiation, which may be used as envelope materials in nuclear shaping. This study reveals some details during spermatogenesis of the fern Pteris fauriei, which contributes to understand the mechanism of spermatogenesis.

Key words: Fern, Pteris fauriei, Spermatogenesis, Ultrastructure

摘要：

超微结构研究显示傅氏凤尾蕨（Pteris fauriei Hieron）精子发生过程包括生毛体、多层结构和鞭毛等运动细胞器重新发生，环状线粒体形成，核塑形等过程，最后形成一个螺旋形的游动精子，这与其他真蕨类精子发生过程相似。本研究观察到的一些新现象包括：精细胞在分化早期呈极性，细胞核位于精细胞的近极端，生毛体、线粒体和质体等细胞器主要分布远极端；在生毛体分化早期，可见大量微管从其发出，其周围线粒体丰富；基体分化经历了前中心粒、中心粒和基体3个阶段，它们的内部结构不同；研究表明生毛体内的不定形物质是微管组织者，多层结构、附属微管带及鞭毛等细胞器均由不定形物质分化形成；精细胞在分化过程中产生了丰富的膜结构，它们可能为精核塑形提供原料。本研究报道了傅氏凤尾蕨精细胞分化的一些细节，这有助于进一步揭示蕨类植物精子发生的细胞学机制。

关键词: 蕨类, 傅氏凤尾蕨, 精子发生, 超微结构

CLC Number:

Q 944

CAO Jian-Guo, DAI Xiao-Fei, WANG Quan-Chi. An Ultrastructural Study of the Spermatogenesis of the Fern Pteris fauriei (Pteridaceae)[J]. Plant Diversity, 2010, 32(4): 303-314.

曹建国, 代小菲, 王全喜. 傅氏凤尾蕨精子发生超微结构的研究[J]. Plant Diversity, 2010, 32(4): 303-314.

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