Abstract: Microtubules are the components of the cytoskeleton in all eukaryotic cells . They are essential for a wide varieties of cellular functions . Most of our knowledge of biochemistry and pharmacology of tubulin has come from extensive studies on mammalian brain microtubule systems, very little information exists on the biochemical and pharmacological properties of plant tubulin and microtubules . We developed an efficient method to prepare enough tubulin in high purity from day lily ( Hemerocallis fulva L .) pollen . Here we reported the ultrastructural morphology of microtubule polymerzied by induction of tubulin taxol in the presence of Mg²⁺ and GTP with transmission electron microscopy .

Key words: Hemerocallis fulva

摘要： 微管(microtubule) 作为细胞骨架的主要成分, 在植物体内, 微管除决定细胞的形状外, 还参与很多重要的细胞功能。但有关微管蛋白生物化学的研究绝大多数来自动物脑组织材料, 对植物微管蛋白的研究除培养细胞外所知甚少, 我们纯化了毫克数量的萱草( Hemerocallis fulva L .) 花粉微管蛋白, 利用紫杉醇作为促进剂, 在Mg2+ 、GTP 等存在下体外聚合成功, 并观察了其电镜下的形态。

关键词: 萱草,  , 微管,  , 微管蛋白,  , 体外聚合, 电镜观察FONT