Abstract: Some species of the genus Paris are of high medicinal value . Severe human activities particularly over-harvesting have led to recent declines of their population size and fragmented habitat patches . Inter-simple sequence repeat markers ( ISSR) were applied to investigate the genetic diversity of eight populations of Paris polyphylla in Yunnan and Sichuan Provinces. Fourteen primers resulted to a total of 251 bands from the eight populations, of which 235 were polymorphic, which showes a high level of genetic diversity in this species . A relatively high level of genetic diversity was revealed: PPB
= 93 . 63% , HE = 0 . 2204 and HO = 0. 3532 at the species level; PPB = 50. 45%, HE = 0.1405 and HO = 0.2194 at the population level. The GST= 0. 3625 indicated a high degree of genetic differentiation among populations . Genetic variation occurred mainly within populations . In UPGMA cluster analysis the six populations of P. polyphylla var. yunnanensis were divided into two clades , and P. polyphylla var. yunnanensis is more related to P. polyphylla var . stenophylla than to P. polyphylla var . pseudothibetica . In this paper , some conservation concerns were discussed which would be helpful for the conservation and sustainable utilization of this medicine resources.

Key words: Paris polyphylla

摘要： 采用ISSR 分子标记对多叶重楼种下3 个变种的8 个居群共208 份样品的遗传多样性进行了分析。l4条引物共检测到251 个清晰的扩增位点, 其中多态位点235 个。在物种水平上, 多态位点百分率(PPB)达93 . 63% , Nei′s 基因多样性指数( HE ) 为0. 2204、Shannon′s 信息指数( HO ) 为0 . 3532。在居群水平上,多态位点百分率(PPB) 为50 . 45% , Nei′s 基因多样性指数( HE ) 为0 . 1405 , Shannon′s 信息指数( HO ) 为0 . 2194 , 这些均表明多叶重楼的遗传多样性水平较高。此外, 还用NTSYS 软件对样品进行了UPGMA 聚类分析, 结果显示滇重楼的6 个居群聚为一支, 滇重楼与狭叶重楼有较近的亲缘关系, 而与长药隔重楼之间的遗传分化较大。本研究提出了合理保护重楼植物资源的若干措施, 为进一步开展滇重楼优质种质引种驯化, 实现种质资源多样性的合理保护和可持续利用提供参考。

关键词: 多叶重楼, ISSR, 遗传多样性FONT