Abstract: A pair of general primer ( ITS5-ITS4) was used to test in amplification of internal transcribed spacer ( ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan. The DNA amplification fragment was purified with DNA Gel Extraction Kit and sequenced by CEQ8000. The result of phylogenetic analyses based on ITS1-5. 8SrDNA-ITS2 sequences showed that B. edulis in Yunnan had high homology with B. aestivalis and B. aereus in Europe. 73 bp and 26 bp DNA characteristic fragments differing from all B. edulis were found in the ITS-region of B. aereus and B. aestivalis respectively.

Key words: Boletus edulis

摘要： 利用ITS 的通用引物(ITS5-ITS4) 对云南的美味牛肝菌( Boletus edulis) 子实体的DNA 进行PCR 扩增, 扩增产物回收后直接测序。序列的聚类分析表明, 在ITS1-5 . 8S rDNA-ITS2 区域, 云南的美味牛肝菌与欧洲的夏牛肝菌( B. aestivalis) 和铜色牛肝菌( B . aereus) 同源性较高, 但在ITS2 区域夏牛肝菌和铜色牛肝菌分别有一段美味牛肝菌没有的大小分别为73 bp 和26 bp 的特征序列。

关键词: 同源性, PCRFONTFONTP