Overexpression of miR396 miRNAs Caused Flower Stigma Curved in Arabidopsis thaliana

doi:10.3724 SP.J.1143.2009.09016

Plant Diversity ›› 2009, Vol. 31 ›› Issue (04): 353-356.DOI: 10.3724 SP.J.1143.2009.09016

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Overexpression of miR396 miRNAs Caused Flower Stigma Curved in Arabidopsis thaliana

LIU Dong-Me^{i1 , 2} , YANG Feng-Xi^{1 ,2} , YU Di-Qiu¹

1 Xishuangbanna Tropical Botanical Garden , Chinese Academy of Sciences, Kunming 650223 , China;
2 Graduate University of Chinese Academy of Sciences, Beijing 100049 , China

Received:2009-01-22 Online:2009-08-25 Published:2009-08-25
Contact: YU Di-Qiu

高表达miR396 小分子导致拟南芥花柱头弯曲

刘冬梅^{1 , 2} , 杨凤玺^{1 , 2} , 余迪求¹

1 中国科学院西双版纳热带植物园, 云南昆明 650223 ; 2 中国科学院研究生院, 北京 100049

通讯作者: 余迪求

Abstract

Abstract: MicroRNAs (miRNAs) are - 21 nucleotide (nt) , endogenous and encoding RNAs that play important regulatory roles in growth and development by targetingmRNAs for direct cleavage or translational repression in plants . To analyze the biological function of miR396, we placed two synthetic precursors, MIR396a or MIR396b , under the control of the enhanced
Cauliflower mosaic virus (CaMV) 35S promoter in a plant transformation vector ( pOCA30) and transformed the constructs into Arabidopsis . Genetic analysis showed that flower stigmas of 35S: MIR396 transgenic Arabidopsis were curve and siliques of miR396 transgenic plants were abnormal. In addition , Northern blot analysis showed that the miR396 miRNAs and levels of MIR396 precursor transcripts were significantly increased in transgenic miR396 plants compared with control. These results strongly supported that miR396 played an important role in plant flower development .

Key words: Arabidopsis thaliana

摘要： MicroRNAs (miRNAs) 是大小约21 个碱基、内源、非编码的小分子RNA。以拟南芥( Arabidopsis thaliana)miR396 小分子为研究对象, 分别克隆到了miR396 小分子的两个前体( MIR396a , MIR396b) , 得到了转基因植株。通过转基因植株的遗传学研究发现, 高表达miR396 小分子导致转基因拟南芥的花柱头弯曲。花柱头的弯曲影响了角果的正常发育。另外, Northern 杂交结果表明转基因拟南芥花部位的miR396 及其前体的表达量与对照相比显著增加。这些结果表明高表达miR396 小分子可以导致拟南芥花柱头弯曲。

关键词: 拟南芥, miR396, 花柱头, 弯曲

CLC Number:

Q 945

LIU Dong-Me^{i ,} , YANG Feng-Xi^, , YU Di-Qiu

. Overexpression of miR396 miRNAs Caused Flower Stigma Curved in Arabidopsis thaliana[J]. Plant Diversity, 2009, 31(04): 353-356.

刘冬梅1 , 2 , 杨凤玺1 , 2 , 余迪求1 . 高表达miR396 小分子导致拟南芥花柱头弯曲[J]. Plant Diversity, 2009, 31(04): 353-356.

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Overexpression of miR396 miRNAs Caused Flower Stigma Curved in Arabidopsis thaliana

高表达miR396 小分子导致拟南芥花柱头弯曲

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