应用SDS-PAGE 分析和PCR 鉴定长发带芒草的高分子量谷蛋白亚基

Plant Diversity ›› 2005, Vol. 27 ›› Issue (06): 653-656.

应用SDS-PAGE 分析和PCR 鉴定长发带芒草的高分子量谷蛋白亚基

颜泽洪, 代寿芬, 刘登才, 魏育明, 郑有良

四川农业大学小麦研究所

收稿日期:2005-04-06 出版日期:2005-10-25 发布日期:2005-10-25
通讯作者: 颜泽洪

Characterization of HMW Glutenin Subunits from Taeniatherum crinitum by SDS-PAGE and PCR

YAN Ze-Hong, DAI Shou-Fen , LIU Deng-Cai , WEI Yu-Ming , ZHENG You-Liang

Triticeae Research Institute , Sichuan Agricultural University , Dujiangyan 611830 , China

Received:2005-04-06 Online:2005-10-25 Published:2005-10-25
Contact: YAN Ze-Hong

摘要/Abstract

摘要： 利用SDS-PAGE 分析、PCR 扩增和序列测定与分析研究了长发带芒草(Taeniatherum
crinitum) 的高分子量谷蛋白亚基及其基因。结果显示, 长发带芒草中发现的高分子量谷蛋白亚基与普通小麦中的类似, 但迁移率存在较大差异。其中, x 型亚基均比Dx2 亚基迁移率小或接近, y 型亚基均比Dx12 亚基迁移率更快。本研究结果揭示了带芒草属具有与普通小麦类似的高分子量谷蛋白亚基, 这些亚基在小麦品质遗传改良中具有潜在的利用价值。

关键词: 带芒草属, , 高分子量谷蛋白亚基, SDS-PAGE 分析, , PCR 扩增, , 序列分析FONT

Abstract: HMW glutenin subunits and genes in Ta. crinitum were investigated by using SDS-PAGE and PCR technology . The results suggested that HMW glutenin in Ta. crinitum were very similar but not identical to those of wheat especially in terms of electrophoresis mobility . The electrophoresis mobility of the x type subunits of Taeniatherum was slower or closer to that of Dx2 of wheat , and of y type subunits were faster than that of Dy12 of wheat . Our results indicated that Taeniatherum species may contain HMW glutenin subunits similar to those of wheat and that the Taeniatherum subunits may have the potential value for improving the end use qualities of wheat .

Key words: Taeniatherum

颜泽洪, 代寿芬, 刘登才, 魏育明, 郑有良. 应用SDS-PAGE 分析和PCR 鉴定长发带芒草的高分子量谷蛋白亚基[J]. Plant Diversity, 2005, 27(06): 653-656.

YAN Ze-Hong, DAI Shou-Fen , LIU Deng-Cai , WEI Yu-Ming , ZHENG You-Liang. Characterization of HMW Glutenin Subunits from Taeniatherum crinitum by SDS-PAGE and PCR[J]. Plant Diversity, 2005, 27(06): 653-656.

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