25S rDNA 在杨属植物染色体上的定位

Plant Diversity ›› 2007, Vol. 29 ›› Issue (04): 423-428.

25S rDNA 在杨属植物染色体上的定位

董凤平¹ , 韩素英² , 张守攻² , 齐力旺²,
刘博¹ , 李秀兰¹ , 陈成彬¹

1 南开大学生命科学学院, 天津 300071; 2 中国林业科学院林业研究所细胞生物学实验室, 北京 100091

收稿日期:2006-12-22 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 陈成彬

Physical Mapping of 25S rDNA on Metaphase Chromosomes of Populus (Salicaceae) in Five Sections by Fluorescence in Situ Hybridization

DONG Feng-Ping¹ , HAN Su-Ying² , ZHANG Shou-Gong², QI Li-Wang² ,
LIU Bo¹, LI Xiu-Lan¹ , CHEN Cheng-Bin¹

1 College of Life Science, Nankai University , Tianjin 300071 , China ; 2 Laboratory of Cell Biology, the Research Institute of Forestry , the Chinese Academy of Forestry, Beijing 100091 , China

Received:2006-12-22 Online:2007-08-25 Published:2007-08-25
Contact: CHEN Cheng-Bin

摘要/Abstract

摘要： 利用荧光原位杂交技术对杨属( Populus) 植物五个组中二倍体( 2n = 2x = 38 ) 代表种: 毛白杨( P. tomentosa) 、箭杆杨( P. nigra var. thevestina) 、大叶杨( P. lasiocarpa)、小青杨( P. pseudo-simonii ) 、胡杨( P. euphratica) ; 以及所发现的白杨组和黑杨组天然三倍体( 2n = 3x = 57 ) : 毛白杨( P. tomentosa) 、武黑1号( P. euramericana cv . Wuhei-1) 进行了25S rDNA 的染色体定位。二倍体毛白杨、箭杆杨、小青杨和大叶杨都具有4 个25S rDNA 位点, 而胡杨只有2 个较大的25S rDNA 定位于1 对小的染色体上, 白杨和黑杨天然三倍体的两个种各有6 个25S rDNA 位点。同时作者还将杨属植物25S rDNA 的分布变化与常规核型分析结果进行了比较。

关键词: 杨属, 荧光原位杂交, 25S rDNA 定位FONT

Abstract: This study examined the physical localizations of the 25S rDNA on the mitotic metaphase chromosomes of seven species in the five sections of Populus, using fluorescence in situ hybridization (FISH) . Four 25 rDNA loci were detected on the chromosomes of five wild diploid species (2n = 2x = 38) , P. tomentosa , P. nigra var. thevestina , P. pseudo-simonii, P. lasiocarpa and P. euphratica . Six 25 rDNA loci were detected on the chromosomes of two wild triploid species (2n= 3x = 57) , P. tomentosa in section Leuce and P. euramericana cv . Wuhei-1 in section Aigeiros . While only one pair of 25 rDNA loci located at the smallest chromosome was detected in P. euphratica chromosomes. Coupled with the approach of common karoytype analysis, this article also discusses the variations of 25S rDNA distribution on Populus chromosomes .

Key words: Populus

中图分类号:

Q 942

董凤平1 , 韩素英2 , 张守攻2 , 齐力旺2 ,
刘博1 , 李秀兰1 , 陈成彬1 . 25S rDNA 在杨属植物染色体上的定位[J]. Plant Diversity, 2007, 29(04): 423-428.

DONG Feng-Ping , HAN Su-Ying , ZHANG Shou-Gong, QI Li-Wang ,
LIU Bo, LI Xiu-Lan , CHEN Cheng-Bin. Physical Mapping of 25S rDNA on Metaphase Chromosomes of Populus (Salicaceae) in Five Sections by Fluorescence in Situ Hybridization[J]. Plant Diversity, 2007, 29(04): 423-428.

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