多叶重楼遗传多样性的ISSR 分析

Plant Diversity ›› 2007, Vol. 29 ›› Issue (04): 388-392.

多叶重楼遗传多样性的ISSR 分析

何俊^{1 , 2} , 杨柏云¹ , 陈少风¹ , 高连明² , 王红²

1 南昌大学生命科学学院, 江西南昌 330047; 2 中国科学院昆明植物研究所
生物多样性与生物地理学重点实验室, 云南昆明 650204

收稿日期:2006-10-09 出版日期:2007-08-25 发布日期:2007-08-25
通讯作者: 王红

Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers

HE Jun^{1 , 2}, YANG Bai-Yun¹ , CHEN Shao-Feng¹ , GAO Lian-Ming² , WANG Hong²

1 Colleage of Life Science , Nanchang university, Nanchang 330047 , China; 2 Laboratory of biodiversity and biogeography ,
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204 , China

Received:2006-10-09 Online:2007-08-25 Published:2007-08-25
Contact: WANG Hong

摘要/Abstract

摘要： 采用ISSR 分子标记对多叶重楼种下3 个变种的8 个居群共208 份样品的遗传多样性进行了分析。l4条引物共检测到251 个清晰的扩增位点, 其中多态位点235 个。在物种水平上, 多态位点百分率(PPB)达93 . 63% , Nei′s 基因多样性指数( HE ) 为0. 2204、Shannon′s 信息指数( HO ) 为0 . 3532。在居群水平上,多态位点百分率(PPB) 为50 . 45% , Nei′s 基因多样性指数( HE ) 为0 . 1405 , Shannon′s 信息指数( HO ) 为0 . 2194 , 这些均表明多叶重楼的遗传多样性水平较高。此外, 还用NTSYS 软件对样品进行了UPGMA 聚类分析, 结果显示滇重楼的6 个居群聚为一支, 滇重楼与狭叶重楼有较近的亲缘关系, 而与长药隔重楼之间的遗传分化较大。本研究提出了合理保护重楼植物资源的若干措施, 为进一步开展滇重楼优质种质引种驯化, 实现种质资源多样性的合理保护和可持续利用提供参考。

关键词: 多叶重楼, ISSR, 遗传多样性FONT

Abstract: Some species of the genus Paris are of high medicinal value . Severe human activities particularly over-harvesting have led to recent declines of their population size and fragmented habitat patches . Inter-simple sequence repeat markers ( ISSR) were applied to investigate the genetic diversity of eight populations of Paris polyphylla in Yunnan and Sichuan Provinces. Fourteen primers resulted to a total of 251 bands from the eight populations, of which 235 were polymorphic, which showes a high level of genetic diversity in this species . A relatively high level of genetic diversity was revealed: PPB
= 93 . 63% , HE = 0 . 2204 and HO = 0. 3532 at the species level; PPB = 50. 45%, HE = 0.1405 and HO = 0.2194 at the population level. The GST= 0. 3625 indicated a high degree of genetic differentiation among populations . Genetic variation occurred mainly within populations . In UPGMA cluster analysis the six populations of P. polyphylla var. yunnanensis were divided into two clades , and P. polyphylla var. yunnanensis is more related to P. polyphylla var . stenophylla than to P. polyphylla var . pseudothibetica . In this paper , some conservation concerns were discussed which would be helpful for the conservation and sustainable utilization of this medicine resources.

Key words: Paris polyphylla

中图分类号:

Q 943
Q 949

何俊1 , 2 , 杨柏云1 , 陈少风1 , 高连明2 , 王红2 . 多叶重楼遗传多样性的ISSR 分析[J]. Plant Diversity, 2007, 29(04): 388-392.

HE Jun^,, YANG Bai-Yun , CHEN Shao-Feng , GAO Lian-Ming , WANG Hong. Assessment of Genetic Diversity of Paris polyphylla (Trilliaceae) by ISSR Markers[J]. Plant Diversity, 2007, 29(04): 388-392.

[1]	赵凯1、2, 王德元2, 张文娟2, 邵剑文2 . 都支杜鹃遗传多样性的ISSR分析[J]. Plant Diversity, 2013, 35(5): 578-584.
[2]	蒋志敏, 陈玉霞, 包颖. 黄河三角洲柽柳居群的遗传结构和遗传分化[J]. Plant Diversity, 2011, 33(4): 403-408.
[3]	马佳梅1 , 2 , 殷寿华1 . 西双版纳地区流苏石斛遗传多样性的ISSR 分析[J]. Plant Diversity, 2009, 31(1): 35-41.
[4]	向振勇1 , 2 , 3 , 宋松泉2 , 王桂娟2 , 陈茂盛2 , 杨成源2 , 龙春林1 . 云南南部不同种源地小桐子遗传多样性的ISSR 分析[J]. Plant Diversity, 2007, 29(06): 619-624.
[5]	汪书丽1 , 2 , 李巧明1 . 中国木棉居群的遗传多样性[J]. Plant Diversity, 2007, 29(05): 529-536.
[6]	王祎玲1, 2 , 赵桂仿1 . 七筋菇自然居群的遗传结构分析[J]. Plant Diversity, 2007, 29(03): 293-299.
[7]	张萍; 董玉芝; 魏岩; 胡成志. 利用ISSR标记对新疆白梭梭居群的遗传多样性分析[J]. Plant Diversity, 2006, 28(04): 359-362.
[8]	索志立，张会金，张治明，陈富飞，陈富慧. 紫斑牡丹与牡丹种间杂交后代的DNA分子证据[J]. Plant Diversity, 2005, 27(01): 42-48.
[9]	廖文芳夏念和邓云飞郑庆衍. 华木莲的遗传多样性研究[J]. Plant Diversity, 2004, 26(01): 1-3.
[10]	肖龙骞葛学军龚洵郑思乡. 贵州苏铁遗传多样性研究[J]. Plant Diversity, 2003, 25(06): 1-3.